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Citations for Promega :
951 - 1000 of 1751 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... pMAD plasmid was also digested with MluI and NcoI and dephosphorylated by addition of TSAP (Promega, streamlined restriction digestion protocol ...
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bioRxiv - Immunology 2021Quote: ... HEK 293T cells were transfected with the pseudovirus encoding plasmids using FuGENE 6 Transfection Reagent (Promega). The virus culture supernatant was harvested at 48h and 72h post transfection and stored at -80°C until use ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids for preparing DNA templates for in vitro transcription were assembled using the pGEM-11Zf(+) (Promega) backbone ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid miniprep DNA isolation was performed using Wizard Plus SV Minipreps DNA Purification System (Promega, USA). Plasmid maxiprep DNA isolation was performed using NucleoBond Extra Maxi Plus EF Kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2021Quote: ... These recombinant plasmids were subsequently used to generate digoxigenin-labelled riboprobes using the Riboprobe System (Promega) with SP6 or T3 RNA polymerases and digoxigenin-labelled Uracil triphosphate (Roche ...
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bioRxiv - Microbiology 2020Quote: The plasmids were transfected into the 293T cells using Fugene HD transfection reagent (Promega, Madison, WI). The culture media containing VLP were harvested 3 days post transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were transiently transfected with the plasmid using FuGENE HD transfection reagent (#E2311, Promega, USA) according to manufacturer’s protocol and GFP+ cells were sorted ...
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bioRxiv - Microbiology 2022Quote: ... Positive cells were multiplied and their plasmid extracted using the Wizard Minipreps DNA purification system (Promega). Plasmids were electroporated into WT B ...
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bioRxiv - Neuroscience 2023Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2022Quote: ... Plasmids were purified from these cultures using a Wizard Plus SV Miniprep DNA Purification kit (Promega). All inserted sequences were confirmed by Sanger sequencing using M13 forward and reverse primers (M13F and M13R ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cells were transfected with 1 or 1.5 μg plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... pHT315-GFP was then extracted and purified using either the Pureyield plasmid miniprep kit (Promega #A1223) or the QIAGEN® Plasmid Mini Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5μg of plasmid was transfected into 6-well dishes using Fugene HD (Promega, Madison, WI, USA) with 3:1 transfection reagent to DNA for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified BAC and plasmid DNA were transfected into HEK293 cells with FuGene HD transfection reagent (Promega) in a 1:3 DNA:FuGene HD ratio ...
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bioRxiv - Biophysics 2023Quote: We stably transfected U2OS cells with this plasmid using Fugene 6 following the manufacturer’s instruction (Promega). α-Amanitin (SIGMA #A2263 ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of each purified plasmid was used as input in a 50 μl TnT (Promega) reaction incubated at 25 °C for 2 h ...
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bioRxiv - Physiology 2023Quote: ... cells were transfected with the indicated plasmids using FuGENE® HD Transfection Reagent (#E2311, Promega Corporation) at a ratio of 1:5 (plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... mouseStoml3-GFP10::mCHERRY and mouseElkin1-GFP11::eBP2 plasmids using Fugene HD and following manufacturer’s instructions (Promega). 7 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen and Promega, respectively) following manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: HTR cells were transfected with the indicated plasmids using TransFast transfection reagent (Promega,Madison, WI, USA) according to the manufacturer’s protocol as previously described [9] ...
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bioRxiv - Immunology 2023Quote: ... 1 ng pEF1α-Renilla and 130 ng plasmid of interest and 0.66 µl FuGENE HD (Promega) diluted to 10 µl total volume with OptiMEM ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were transfected using 2 µg of plasmid DNA and 6 µL of FuGENE (Promega) for gene editing or using a NEPA21 electroporation system (Nepa Gene ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transiently transfected with 1.5 μg of plasmid using FuGENE 6 transfection reagent (#E2692, Promega), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The linearized plasmid was then purified using the Wizard Plus SV Miniprep DNA Purification System (Promega), and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 µg of plasmid and 2 µg of carrier DNA were combined with FuGENE HD (Promega) at a 3:1 ratio and added per well of a 6-well plate seeded with 70% confluent HEK293 cells ...
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bioRxiv - Biophysics 2023Quote: ... cells were transiently transfected using 1g of plasmid and 3ul of FuGENE HD transfection reagent (Promega) based on manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Other plasmids were obtained from commercial vendors or from the Addgene repository: pRL-CMV-Renilla (Promega), pRS0045 (Addgene #131124) ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Cell Biology 2024Quote: Transfection of plasmids into HT-1080 derivative cell lines was carried out using Fugene HD (Promega) according to manufacturer guidelines ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Developmental Biology 2024Quote: ... The membranes were washed 3x 10 minutes in TBS-T before being incubated for 2 hours with the secondary anti-mouse-HRP- or anti-rabbit HRP antibody (1:5000, Promega anti-mouse, W4021; anti-rabbit W4011). The membranes were washed in TBS-T for 3x 10 minutes and developed with Western Lightning Plus-ECL reagent (Perkin Elmer NEL104) ...
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bioRxiv - Biochemistry 2019Quote: ... and HRP-conjugated rabbit anti-mouse antibody 1:10,000 (Promega, W4028). The plate was developed with 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid ...
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bioRxiv - Genomics 2019Quote: ... and anti-rabbit-HRP or anti-mouse-HRP 1:10000 (Promega). Blots were visualised with the Fusion Fx7 system and quantified with ImageJ 1.48k.
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Microbiology 2021Quote: ... and goat anti-mouse IgG-HRP (Promega W4021; 1:5,000 dilution).
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bioRxiv - Microbiology 2020Quote: ... Secondary -mouse IgG-HRP conjugate (Promega, Mannheim, Germany; 1:4,000 dilution) was used for detection ...
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bioRxiv - Biochemistry 2022Quote: ... The SNRPN promoter was amplified from mouse genomic DNA (Promega, G3091) using design primers (Supplementary Table 3) ...
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bioRxiv - Microbiology 2022Quote: ... and an anti-mouse IgG HRP conjugate secondary antibody (Promega, #W4021) (13) ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-β-galactosidase (1:1000, Z3783, Promega, Madison, WI, USA), rabbit anti-β-galactosidase (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... end/or donkey anti-mouse 488 (W4021 – Promega, Madison, WI, USA) IgG ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-β-galactosidase (Z3781; Promega, Madison, WI; 1:200). Alexa Fluor 488-conjugated (A21206 ...
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bioRxiv - Cell Biology 2020Quote: ... Alkali-phosphatase (AP)-conjugated anti-mouse or anti-rabbit IgG (Promega) as well as AP substrate consisting of 5-bromo-3-chloro-indolyl phosphate (Roche Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... both purchased from Life technologies and (iii) an anti-mouse conjugated with HRP (#W402B) from Promega. For light or electron microscopy ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was treated with anti-mouse IgG HRP conjugate (Promega) and washed 3 times with phosphate buffered saline ...
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bioRxiv - Developmental Biology 2022Quote: ... from mouse genomic DNA and PCR products cloned into pGL4.23 (Promega) pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with HRP-conjugated donkey anti-mouse IgG (1:10,000; Promega), and revealed with Super Signal chemiluminescent substrate reagents (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... and anti-mouse IgG HRP conjugate (W4021; Promega, Madison, WI, USA) as secondary antibodies for 1.5 to 2.0 h at room temperature with gentle shaking ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-mouse IgG-horseradish peroxidase (HRP) conjugate (1:3,000 Promega) was used as secondary antibody ...