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Citations for Promega :
1401 - 1450 of 1751 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... a pPolI-Firefly plasmid encoding the Firefly luciferase sequence in negative polarity flanked by the 5’ and 3’ non-coding regions of the IAV NS segment was used and the pTK-Renilla plasmid (Promega) was used as an internal control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was co-transfected at a 1:5 ratio with either the HaloTag-ubiquitin plasmid (expressing Ub-Halo) or the HaloTag control (Promega). Transfected cells were incubated for 20 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Shigella conjugants that grew at 30°C and were Cb60 resistant were screened by PCR for the presence of lpxE and the large virulence plasmid using GoTaq (Promega) and primer sets lpxE-F/lpxE-R (lpxE ...
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bioRxiv - Molecular Biology 2023Quote: ... Flp-In T-REX HeLa cells were transfected with 1.8 μg of pOGG4 plasmid and 200 ng of pcDNA5/FRT/TO plasmid encoding either a GFP-BICD2N-MTS or a GFP-MTS cassette using FuGENE HD (Promega). Cells with a stably integrated transgene were selected with 150 μg/mL hygromycin over 4-5 days ...
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bioRxiv - Cancer Biology 2023Quote: ... We co-transfected cells with the Tol2 GIV transposon and a Tol2 transposase (Vector Builder) at a 3:1 ratio of micrograms of plasmid DNA using Fugene 6 (Promega) according to the manufacturer’s directions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus was generated by transfecting the Plat-E packaging cell line with 10 µg plasmid DNA and 30 µl FuGene HD transfection reagent (Promega). Viral supernatants were collected from transfected Plat-E cells at 40 ...
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bioRxiv - Cell Biology 2023Quote: ... S2R+ cells were grown in SF-4 medium and cotransfected with desired plasmids using FuGENE® HD transfection reagent (Promega). Specifically ...
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bioRxiv - Bioengineering 2023Quote: ... Transfection was performed with 1 μg of plasmid DNA in combination with 3 μL of Fugene HD transfection reagent per well (Promega).
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bioRxiv - Biochemistry 2023Quote: ... In brief: BRD4 bromodomains and full-length HDACs were obtained as plasmids cloned in frame with a terminal NanoLuc fusion (Promega). Plasmids were transfected into HEK293T cells using FuGENE HD (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... A 240 base pair sequence of the human gastrin gene [25] was cloned upstream of the firefly luciferase-encoding sequence in the pGL3B reporter plasmid (Cat #E1751, Promega). Upon reaching 60–75% confluency in 6-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown to 80% confluency prior to transfection with the indicated FLAG tagged ISG15 pcDNA5 plasmids using the FuGene transfection reagent (Promega). After 24 hr ...
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bioRxiv - Biochemistry 2023Quote: ... by transfection of the pSpCas9(BB)-2A-Puro (PX459) plasmid containing A3B gRNAs with FuGENE 6 Transfection Reagent (E2691; Promega). 16h after transfection ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced using 5 µg of plasmids in 25 µL in vitro translation reaction of TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega). For the OSH15-RI complex ...
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bioRxiv - Biochemistry 2024Quote: ... The FXR DBD (residues 120-196) and the hinge region (residues 197-243) were cloned into the pACT plasmid (Promega) to generate fusion proteins with the VP16 activation domain ...
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bioRxiv - Cell Biology 2024Quote: ... HA-dTAG-RBM7 cells were transfected with pB[MYC-RBM7x] BSD plasmids along with a pB transposase expressing vector (pBASE) using Viafect transfection reagent (Promega). Cell pools were selected using BSD for 7-10 days or until negative control cells no longer survived ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were then plated onto 35 mm glass coverslips pre-coated with poly-D-lysine (100 g/mL) and transfected with 300 ng of each plasmid using FuGene transfection reagent (Promega) or Lipofectamine 2000 (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... a pPolI-Firefly plasmid encoding the Firefly luciferase sequence in negative polarity flanked by the 5’ and 3’ non-coding regions of the IAV NS segment was used (Krischuns et al., 2022) and the pTK-Renilla plasmid (Promega) was used as an internal control ...
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bioRxiv - Biochemistry 2023Quote: ... pLH3/DTX3L or pLH3/DTX3LΔN) and accessory plasmids pMD2g and psPAX2 (ratio 2:1:1) using ViaFect transfection reagent (Promega E498A). After cell incubation at 37°C for ∼16 h ...
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bioRxiv - Biochemistry 2023Quote: The assay was performed as described previously.32 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Supplementary Table S3 ...
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bioRxiv - Microbiology 2023Quote: The region upstream of the genes of interest was amplified by PCR with proper primers (Table S4) and cloned into the pGEM-T easy plasmid (Promega). The insert was removed by digestion and subcloned into the pRKlacZ290 vector to generate transcriptional fusions to the lacZ gene ...
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bioRxiv - Biochemistry 2023Quote: The assay was performed as previously described.61 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Table S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase assays were performed by co-transfecting HEK-293T cells with 100 ng pCMV6-empty or pCMV6-miR-497/195 with 10 ng of psiCHECK2 reporter plasmids using FuGENE6 (E2691; Promega) and performing the Dual-Luciferase Reporter Assay System (E1910 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... and then transfected with the plasmid encoding GFP-TFEB or mCherry-GFP-LC3 using FuGene HD transfection reagent (Promega, WI), following manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... carrying the non-risk or risk allele of each SNP (Supplemental Table 4) were cloned into the no-promoter firefly luciferase reporter plasmid pGL4.14 [luc2/Hygro] (Promega, #E6691) or minimal promoter firefly luciferase reporter plasmid pGL4.26 [luc2/miniP/Hygro] (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... 20,000 cells were seeded in 12-well plates and transfected with 300ng of pCAG-Mest or pCAG-GFP control plasmid using FuGENE HD transfection reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... the pRL plasmid acts as a transfection control with Renilla luciferase driven by an upstream CMV enhancer and CMV promoter (Promega). Test enhancers were cloned by either PCR of genomic DNA with primers containing NheI and XhoI restriction sites ...
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bioRxiv - Immunology 2024Quote: ... dsFluc and dsGFP were produced from a T7 and SP6 promoters sequences containing the firefly luciferase sequence from pGL3-Basic plasmid (Promega) and GFP PCR amplification from plasmid pDSAG (Addgene #62289 ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of reporter vector plus 50 ng of the renilla luciferase plasmid was transfected to cells using FuGENE 6 (Promega); cells were then treated with DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... was cloned upstream a SV40 minimal promoter and a luciferase reporter gene in the backbone of a PGL3 plasmid (Promega). We used lipofectamine 2000 to transfect p53-/- MEFs with 2 μg of either luciferase expression vector ...
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bioRxiv - Cancer Biology 2024Quote: ... Each well was transfected with 500 ng pGL4.22 vector with or without H1-0 promoter expression as well as 5 ng Renilla luciferase control plasmid pGL4.73 (Promega, #E6911), and 250 ng of the respective pcDNA3.1 vectors (Thermo Fisher Scientific ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The annealed oligos were ligated into the Xho I and Not I sites in the 3’UTR of the Renilla luciferase gene in the psiCHECK-2 plasmid (C8021, Promega). Each plasmid was transfected individually into HEK 293 cells with miR-891b using DharmaFECT 1 Transfection Reagent (T-2001-03 ...
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bioRxiv - Cell Biology 2024Quote: Correlative light and electron microscopy was conducted as previously described.61 PDZD8-Halotag knocked-in HeLa cells were overexpressed with plasmids encoding Venus-FKBP8N403K or YFP-ActA and then treated with 200 nM Janelia Fluor 549 dye (Promega) for 20 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were then cloned into the BamHI restriction site of luciferase T7 control DNA plasmid (Cat. No. L4821, Promega). Modifications of uORFs or other mutations were performed using the Q5 site-directed mutagenesis kit (Cat ...
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bioRxiv - Synthetic Biology 2024Quote: ... Strings and plasmid backbones were amplified by PCR followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega). Plasmids were assembled using isothermal GibsonAssembly® reaction (NEBuilder® HiFi DNA Assembly Master Mix).
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bioRxiv - Molecular Biology 2024Quote: ... + 0.5 μg of “reporter” plasmid (pJBM560 and derivatives thereof) + 0.25 μg of pCEP/GFP using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Plant Biology 2024Quote: ... and 1ug of plasmid DNA was used for in vitro protein expression using the TNT rabbit reticulocyte expression system (Promega) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... We used the following primary antibodies: mouse anti-β-galactosidase (Promega, 1:1,000 dilution), rabbit anti-GFP (MBL,1:1,000 dilution) ...
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bioRxiv - Immunology 2021Quote: ... mouse tail DNA was amplified using GoTaq G2 Green Master Mix (Promega, cat. M7823) or KAPA Taq PCR Kit (Takara Bio ...
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bioRxiv - Cell Biology 2020Quote: ... The peroxidase-conjugated secondary antibodies were goat anti-mouse IgG (715-035-151, Promega) and anti-rabbit IgG (711-035-152 ...
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bioRxiv - Molecular Biology 2019Quote: ... Horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit IgG (Promega; 1:10,000) was incubated for 1 h at room temperature and then washed four times with PBS-Tween for 20 min ...
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bioRxiv - Plant Biology 2019Quote: ... The secondary antibody was an anti-mouse coupled to HRP 1:10,000 (W4021, Promega). Detection was performed with the Pico Plus system (34580 ...
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bioRxiv - Cell Biology 2019Quote: ... As secondary antibody a mouse IgG HRP conjugate was used (Promega, Madison, WI, USA). Detection was carried out by using AceGlow (VWR Peqlab ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were probed with either goat anti-mouse IgG-HRP (Promega, catalogue no. W4021) or goat anti-rabbit IgG-HRP (catalogue no ...
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bioRxiv - Cell Biology 2021Quote: ... HRP conjugated anti-rabbit (W401B) and anti-mouse (W402B) secondary antibodies were from Promega. CB-5083 was a gift from Cleave Biosciences and Bortezomib was from Selleckchem ...
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bioRxiv - Microbiology 2021Quote: ... Anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were purchased from Promega (USA). Alexa Fluor 488 and Alexa Fluor 594 antibodies were purchased from Invitrogen (USA) ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HPR-conjugated secondary antibodies were from Promega. Anti-Mouse Alexa Fluor 488 (A32790) ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HRP conjugated secondary antibodies were from Promega. Anti-mouse Alexa® Fluor 488 (#A32790 ...