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Citations for Promega :
1151 - 1200 of 4810 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... CellTiter 96® AQueous One Solution Assay (Promega, Southampton, UK); Human CD34+ Progenitor Cells (hCD34+-CB ...
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bioRxiv - Molecular Biology 2020Quote: ... using CellTiter 96 AQueous one solution cell proliferation assay (Promega) following manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... then 50 μL One-Glow Luciferase reagent (Promega Corp; E6120) were added into each well ...
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bioRxiv - Cancer Biology 2023Quote: ... The CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) was used and 96 well plates were read using a CLARIOStar microplate reader (BMG Labtech).
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bioRxiv - Cancer Biology 2023Quote: ... Quantus fluorometer and Quanti Fluor ONE dsDNA Kit (Promega, E4870) and 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... One tube was further digested with thermolysin (Promega, Madison, WI) by resuspending the tryptically digested peptides with a solution containing 50 mM Tris-HCl pH 8 and 0.5 mM calcium chloride and adding 1 μg of thermolysin ...
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bioRxiv - Molecular Biology 2023Quote: ... and the QuantiFluor® ONE dsDNA System (Cat# E4871, Promega). When carry-over of full-length plasmid can’t be excluded ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then used in a one-step RTqPCR (Promega) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the ONE-Glo™ Luciferase Assay System (E6110, Promega) exactly as described in 16.
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bioRxiv - Molecular Biology 2021Quote: ... 1 to 2 µg of RNA was used for DNase treatment as per the protocol provided by the manufacturer (Promega, Madison, Wisconsin, USA). This was followed by cDNA synthesis using reverse transcriptase kits from Applied Biosystems (High capacity cDNA synthesis kit ...
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bioRxiv - Microbiology 2021Quote: ... The protein solution was further diluted down to less than 2 M urea with 50 mM Tris-Cl (pH 8) and incubated with 2 μg of modified trypsin (w/w, 1∶50) (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Systems Biology 2024Quote: ... the samples were diluted with 50 mM HEPES (pH 8.5) to reduce urea concentration to 2 M and further digested overnight with trypsin (Promega, 1:50 w/w) at RT ...
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bioRxiv - Molecular Biology 2020Quote: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... Plates were then incubated at 37°C and 5% CO2 for 48 hours upon which cell viability was assessed by adding CellTiter-Glo® (Promega) to the reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... 25uL of reconstituted CellTiter-Glo Luminescent Cell Viability reagent was added to each well and incubated for 5 minutes (Promega, G7571). Luminescence readings were taken using the BioTek Synergy H1 Hybrid Reader ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... at 3500 cells/well and cell proliferation over 3-5 day periods was determined by measuring luminescence with CellTiter-Glo® assay kit (G7570, Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... Individual reactions conducted with UGA-CPES consisted of a 10 µL mixture containing 5 µL of GoTaq Green Master Mix 2X (Promega Corporation), 0.5 µL of template DNA ...
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bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
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bioRxiv - Biochemistry 2022Quote: The crRNA (5’-AAUUUCUACUGUUGUAGAUGUGAUAAGUGGAAUGCCAUGUGGG-3’) was synthesized and purified using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega). The following DNA templates were used for the in vitro transcription reaction ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 5 mL of overnight cultures using the Wizard Genomic DNA Purification Kit (Promega Corp., Madison, WI, USA) and DNA concentration was measured using a Qubit™ Fluorometer and Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Bright-Glo reagent was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega, USA), with titers given in Relative Light Units (RLU ...
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bioRxiv - Microbiology 2021Quote: ... for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega, E1501).
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bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Microbiology 2020Quote: ... Amplifications were performed in a 50 µl final volume using 5 µl of DNA template and 2.5 units of GoTaq polymerase (Promega, Madison, WI). Final concentrations of MgCl2 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... miR159 and U6 RNA (see Table S3) were 5’ end-labelled with [γ-32P]ATP using T4 polynucleotide kinase (PNK) (Promega).
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was again removed and replenished with 30 mL phenol-red free Opti-MEM supplemented with 5 mM CT-TAMRA (Promega, G8251), except for no CT-TAMRA control wells ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were vigorously shaken at 80 rpm for 5 min prior to adding 75 µl of CellTiter-Glo 3D Reagent (Promega #G968B) to each well ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted 5 folds with distilled water and used in a reaction mix composed of 1x GoTaq qRT-PCR master mix (Promega, USA) and 10 nM primers for RT-qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... All PCR amplifications were assembled on ice in 25 μl reactions using 5 μl of 5X Green GoTaq buffer (Promega M3001), 0.5-1 μl of each primer (10 μM) ...
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bioRxiv - Microbiology 2022Quote: ... the PCR was performed in a total volume of 25 μl containing 5 X GoTaq® buffer (Promega, Madison, WI, USA), 2.5 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... DDX60 (Fw 5’- AAGGTGTTCCTTGATGATCTCC-3’ Rv : 5’ -TGACAATGGGAGTTGATATTCC-3’) as analyzed by semiquantitative PCR using the SYBR Green assay GoTaq® qPCR Master Mix (Promega) with standardized primers (Metabion) ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Biophysics 2023Quote: DNA dT70 was diluted to ∼100 pM in buffer (50 mM Tris-acetate pH 7.7, 5 mM Mg-acetate, 50 mM Na-acetate) and SSB (Promega Corporation, USA) was supplemented in specified concentrations (Tab ...
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bioRxiv - Immunology 2023Quote: ... Plates were incubated for 22 hours at 37 °C/5 % CO2 prior to addition of 33ul Bio-Glo reagent (Promega G7940), then luminescence was read on a Clariostar plate reader.
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bioRxiv - Cell Biology 2023Quote: ... reduced with 5 mM DTT and alkylated with 20 mM acrylamide.23 The reaction was quenched by addition of 5 mM DTT and 1.5 µg of trypsin (Promega, Madison, WI) was added to the sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... a calibration curve for SAH was constructed in two independent experiments using from 0 to 5 μM SAH according to the manufacturer’s protocol of the MTase-Glo kit (Promega, Wisconsin, USA). The signal was detected using a Tecan Spark microplate reader (Tecan Japan Co. ...
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bioRxiv - Biochemistry 2023Quote: ... Excess iodoacetamide was quenched by addition of 5 mM DTT and samples were then digested by trypsin digestion (Sequencing Grade Modified Trypsin, Promega Corp) at 37°C for 6 hours in a Thermomixer with shaking (1000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken horizontally at 500 RPM for 5 min before addition of 25 µL of ready-to-use Nano-Glo substrate (Promega; N1130) for measuring luciferase intensity.