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Citations for Promega :
1101 - 1150 of 1622 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was extracted from a 300 μL plasma aliquot using the Viral Total Nucleic Acid Kit (Promega. Madison. WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega ...
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bioRxiv - Immunology 2023Quote: ... viral RNA (vRNA) was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison WI). Next ...
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bioRxiv - Plant Biology 2023Quote: The HLB1 coding sequence corresponding to the N-terminal 200 amino acids was amplified by PCR using the following primers and was cloned into pGEM-T-easy vector (Promega). The NdeI and XhoI fragment of HLB11-200 was cloned into pET28a vector (Novagen ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 1,200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends in pCI mammalian expression vector (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (11668019 ...
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bioRxiv - Immunology 2024Quote: ... Ten to twenty 10 µm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) with the appropriate sample kit and according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Trypsin was reconstituted in 50 mM acetic acid in water at a concentration of 1 μg/μL (Trypsin Gold, Promega). Reconstituted trypsin was added to 50 mM NH4HCO3 in a 1:50 ratio (v/v) ...
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bioRxiv - Cancer Biology 2024Quote: ... dye was withdrawn from stained colonies with 10% acetic acid and the associated absorbance was measured using a microplate reader at 600 nm (GloMax Explorer system, Promega)(45).
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bioRxiv - Cancer Biology 2024Quote: ... Ten to twenty 10 µm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) and Maxwell RSC simplyRNA tissue kit according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 1 μL DNA and 5 μL of Master Mix 2x (Promega) (0.3 units of Taq Polymerase ...
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bioRxiv - Genomics 2020Quote: ... containing 5 μL of GoTaq Master Mix (Promega, Mdison, WI, USA), 1 mM each of forward and reverse primer ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μg/mL Mass Spec Trypsin/Lys-C Mix (V5073, Promega) and 1 mM DTT was added to the samples ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL Tris-phosphate buffer containing 1:50 Nanoglo substrate (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2022Quote: ... or 5-10% (w/vol) PEG8000 (Promega; radius of gyration (Rg) ∼3-5 nm (Cole & Ralston ...
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bioRxiv - Microbiology 2022Quote: ... which is approximately a 5:1 ratio of effector cells (Promega) to target cells ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Physiology 2021Quote: ... and 5 μg lentiviral construct with 30 μL Fugene 6 (Promega) the following day ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were digested with 5 μg of trypsin (Promega, Cat# V5280) at 37 °C overnight under agitation ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM EDTA) supplemented with 1X Protease inhibitor cocktail (Promega G6521) and PhosSTOP phosphatase inhibitor (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl of 50 pmol/µL biotinylated oligo-dT probes (Promega), and 408 µl of diffusion buffer (3X Saline-Sodium Citrate (SSC) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.25 µL of GoTaq® DNA Polymerase (5 U/µL, Promega) and 28.25 µL of ultra-pure water were added into the tubes ...
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bioRxiv - Plant Biology 2023Quote: ... 0.25 µL of GoTaq® DNA Polymerase (5 U/µL, Promega), and 27.75 µL of ultra-pure water ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with 5 μM Z-VAD-FMK (Promega G7231) or DMSO vehicle control ...
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bioRxiv - Microbiology 2023Quote: ... and then 50 μl of 5% (v/v) SteadyGlo reagent (Promega) in glycine buffer (50 mM glycine ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of 5 ng·μl−1 trypsin (sequencing grade, Promega®) in 50 mM ABC ...
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bioRxiv - Biochemistry 2023Quote: ... 5% polyacrylamide TBE gel and stained with Diamond DNA stain (Promega). Experiments were performed with H ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation reactions were carried out with 5 ul of either supplemented rabbit reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from lung tissue using the Maxwell automated instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and quality were measured with HS Total RNA 15nt.methods (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested from Calu-3 cells and inactivated by addition of lysis buffer (Maxwell 16 viral total nucleic acid purification kit, Promega #AS1150) complemented with proteinase K ...
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bioRxiv - Microbiology 2021Quote: RNA from lysed and heat-inactivated samples was purified according to the instructions of the manufacturer using Maxwell 16 viral total nucleic acid purification kit (Promega, #AS1150) or Maxwell 16 LEV simplyRNA cell kit (Promega ...
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bioRxiv - Microbiology 2022Quote: Total RNA from each lung homogenate and nasal wash was isolated using the Maxwell RSC Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega Corporation), following the manufacturer’s suggested protocol ...
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bioRxiv - Microbiology 2023Quote: ... the ladder region was cut off and stained for 30 min in the running buffer containing 10000x diluted Diamond™ Nucleic acid dye (Promega). The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+ ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
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bioRxiv - Biochemistry 2024Quote: ... The gels were imaged using ChemiDocTM MP Imaging system using the Rhodamine filter and then counterstained with DiamondTM Nucleic Acid dye (Promega Corporation) to visualize the primer bands ...
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bioRxiv - Immunology 2021Quote: ... vRNA was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit on the Maxwell 48RSC instrument (Promega, Madison WI). vRNA was then quantified using a highly sensitive qRT-PCR assay based on the one developed by Cline et al.99 ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Pathology 2022Quote: ... or 300 uL urine+DMSO using the Maxwell RSC Viral Total Nucleic Acid Purification Kit on the Maxwell 48 RSC instrument (Promega, Madison, WI).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction mix in a final volume of 83 µl has been prepared by adding 21 µl of the Amino Acid Mixture (for a final concentration of 0.13 mM) and 80 units of RNasin (Promega Ref. number N2511) to 60 µl of RRL ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2023Quote: Total nucleic acid extraction from swab and tissue samples was performed using ReliaPrep™ Viral TNA Miniprep System (Promega, Madison, WI, USA). Swab samples were simply inverted in the tube and briefly centrifuged prior to extraction ...
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bioRxiv - Pathology 2024Quote: ... amniotic fluid and eluates from swab samples) using the Viral Total Nucleic Acid Purification Kit for the Maxwell RSC instrument (Promega, Madison WI) with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the Maxwell 16 LEV simply RNA tissue kit in combination with a Maxwell nucleic acid extraction robot (Promega, WI, USA) for rabbit caecal content ...
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bioRxiv - Cell Biology 2024Quote: ... five µg protein were reduced by 2.5 mM dithiothreitol for 30 min at 37°C and alkylated with 10 mM iodoacetic acid for 30 min at 37°C in the dark before digestion by trypsin (Promega, Walldorf, Germany) in a protein to enzyme ratio 25:1 overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: To analyze the 5’ UTR variants we used the pGL3-Basic (Promega), amplification of the 1.4 kb of the 5’ UTR region was carried out using Phusion High Fidelity polymerase (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µl of 5X GoTaq Flexi reaction buffer (Promega, Madison, WI, USA), 2 mM of MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... the concentrated peak fractions were incubated with 5 μM Halo-Alexa488 (Promega) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 5 μM coelenterazine h (Promega, Madison, WI, USA) in phosphate-buffered saline (PBS ...