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Citations for Promega :
1251 - 1300 of 1622 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
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bioRxiv - Plant Biology 2024Quote: ... The PeSPL6 and PeSPL13a 5’-ends were amplified by PCR using the GoTaq Master mix (Promega), the forward 5’ Primer from GeneRacer kit and the reverse Gene Specific Primer (GSP) ...
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bioRxiv - Biophysics 2024Quote: ... gel plugs were crushed into small pieces and 5–10 μg of sequencing-grade trypsin (Promega) per ∼100 μg protein were added ...
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bioRxiv - Systems Biology 2023Quote: ... Half of the lysis was treated with 5 µl of 10 mg/ml Rnase (Promega:527491) and both lystates (+/- RNase ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 before measurement of luciferase activity using Nano-Glo® Luciferase Assay (Promega, Cat # N1110) and Tecan Spark plate reader.
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... At DIV12 cells are lysed in Passive lysis buffer (diluted 1:5 in MilliQ-water, Promega) for 15min and dual luciferase assay performed using home-made reagents according to Baker and Boyce (63 ...
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bioRxiv - Cancer Biology 2023Quote: ... Digests were diluted further to a 1.5 M urea concentration and 5 µg of trypsin (Promega) was added for 6 hours at 37°C ...
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bioRxiv - Genomics 2023Quote: ... Samples were then allowed to cool for 5 minutes and 5μl of RNase (DNase-free; Promega) was added and incubated at 37ºC for 10 minutes ...
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bioRxiv - Plant Biology 2024Quote: RNAs including 5’ and 3’ UTRs were synthesized in vitro using T7 RNA polymerase kit (Promega). cDNA from A ...
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bioRxiv - Biophysics 2024Quote: ... we applied 100 μl of 1:5 diluted cell culture lysis reagent (Promega, Cat. No.: E1531) to each well ...
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bioRxiv - Plant Biology 2024Quote: ... Reaction mixtures of 10 μl were composed of 5 μl GoTaq qRT-PCR Master Mix (Promega), 0.2 μl of each primer (10 μM) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Plant Biology 2020Quote: ... using 3 - 5 μg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 5 mM iodoacetamide for 30 min in the dark and digested overnight with trypsin (Promega; 1 μg for cell-surface-enrichment samples ...
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and after 5 h the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The embryos were then incubated in a 1:5,000 dilution of TMR HaloTag Ligand (5 mM; Promega) in Danieau’s medium ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 5.5μL of a mix containing 5% SDS and 0.25M EDTA and deproteinized with proteinase K (Promega) for 1hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... membranes were incubated in a 0.165 mg/ml BCIP (5-bromo-4-chloro-3-indolyl phosphate, Promega)/ 0.33 mg/ml NBT (p-nitroblue tetrazolium chloride ...
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bioRxiv - Molecular Biology 2022Quote: ... the HIV 5’ LTR from the pNL4-3 isolate (Genbank AF324493) was cloned into pGL3-Basic (Promega) via Gibson assembly (NEB 2X HiFi ...
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bioRxiv - Genetics 2020Quote: ... fragments of varying sizes (Supplemental Table 5) were cloned into the promoter-less pGL3-basic vector (Promega) using NheI and NcoI sites ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL (20x working stock of NanoBRET Tracer K5 [20 μM] in Tracer Dilution Buffer (Promega N291B)) was added to each well with the exception of the “no tracer” control wells ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and were reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... 5 µL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... and plates were incubated for 5 days before being assayed using the Cell Titer-Glo kit (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... Following 5 days of drug incubation cell viability was assayed using 30μl of CellTiter-Glo® (Promega). Screens were performed in technical triplicate.
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bioRxiv - Plant Biology 2022Quote: ... or with the same buffer supplemented with 5 U of calf intestine alkaline phosphatase (CIAP) from Promega. Samples were incubated at 30°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of LHB promoter-containing reporter vector were transfected with 5 ng of pRLRenilla (#E2231, Promega), with or without 50 ng of pCMV6-NR2F2-WT or p.Arg246His and/or 5 ng of pCMX-NR5A1 ...
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bioRxiv - Genetics 2024Quote: ... then a 5 min final extension (72 °C)—was carried out with GoTaq Green Master Mix (Promega). Deletion loci from mutant flies were amplified using the genotyping primers above with four GoTaq Green PCR reactions per line ...
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bioRxiv - Microbiology 2023Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Biochemistry 2023Quote: A mixture of 10 ng of biotinylated BSA digest and 5 µg of K562 cell digest (Promega) was added to streptavidin beads (10 µL of suspension used ...
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...
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bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
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bioRxiv - Plant Biology 2024Quote: ... The secondary antibody (Goat anti-rabbit IgG HRP, Promega, diluted 1:133333 in 5% milk in PBS) was then applied for 50 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... that included (for 1 well): 5 μl of the SYBR Green Real-Time PCR master mix (Promega), 1.25 μl of the forward and reverse primer (standardized KiCqStartTM primers ...
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bioRxiv - Microbiology 2020Quote: ... 5 % CO2 for 72 h before being washed with PBS and 200 µL Passive Lysis buffer (Promega, UK) added to each well ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Biochemistry 2020Quote: ... After the addition of 5 mM ATP the reaction was taken to a plate reader (Promega GloMax Navigator) and the luminescence collected for approximately 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... entire gel lanes were excised into 5 equal regions from top to bottom and digested with trypsin (Promega) as described (30) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 before quantitative analysis by qPCR was done using GoTaq qPCR Master Mix (Promega) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Ten nanograms of template DNA was added to 5 µl Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units GoTaq DNA Polymerase (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 μl 5x PNK pH 6.5 buffer [350 mM Tris-HCl pH 6.5, 50 mM MgCl2, 5 mM DTT], 1 μl PNK enzyme [NEB], 0.5 μl RNasin [Promega] ...
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bioRxiv - Plant Biology 2021Quote: ... The powder was resuspended in 30 mL of Extraction Buffer 1 (10mM Tris-HCl pH8; 0.4M sucrose; 10 mM MgCl2; 5 mM BME; 0.2 mM PMSF; RNasin PROMEGA) and was filter thought a Nylon membrane of 150 μm and centrifuged at 2000 g for 20 min at 4 °C ...