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Citations for Promega :
801 - 850 of 1661 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Parental MDA-MB-231 cells were transfected with pBABEpuro-ERBB2 plasmid using FuGENE HD (Promega) as per the manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Linearised plasmids were used as template for in vitro transcription using T7 RNA polymerase (Promega) and a ribonucleotide set (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Linearised plasmids were used as template for in vitro transcription using T7 RNA polymerase (Promega) and biotin RNA labeling mix (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... a total of 200 ng plasmid DNAs including (1) pGL4.53 vector encoding Fluc gene (Promega), (2 ...
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bioRxiv - Plant Biology 2021Quote: ... DNA from selected plasmids was extracted using Wizard Plus SV minipreps DNA purification system (Promega) and sequenced using the M13F primer ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with plasmid DNA using FuGENE 6 according to the manufacturer’s instructions (Promega). Cells were fixed with 4% formaldehyde in PBS and permeabilised in 0.5% (v/v ...
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bioRxiv - Physiology 2019Quote: ... a ligation reaction was performed between the pGEM®-T Easy plasmid (Promega Milan, Italy) and the amplicon purified ...
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bioRxiv - Biophysics 2019Quote: ... A total of 3 μg plasmid and 9 μL of FuGENE HD Transfection Reagent (Promega) were delivered into each well following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid libraries were harvested using the Wizard® Plus SV Minipreps DNA purification Kit (Promega). Plasmid libraries were subsequently electroporated to E ...
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Structural variant selection for high-altitude adaptation using single-molecule long-read sequencingbioRxiv - Genomics 2021Quote: ... Seg4: 800-1800 bp) were cloned into the pGL3-control plasmid (Promega, Madison, WI, USA), upstream of the SV40 promoter and the firefly luciferase reporter gene ...
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bioRxiv - Biochemistry 2019Quote: HEK293 was used for transfection of plasmids with FuGENE HD Transfection reagent (Promega, Tokyo, Japan). RIPA buffer was used for obtaining proteins from whole cells ...
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bioRxiv - Genomics 2019Quote: ... RNA probes were labeled by in vitro transcription of plasmids with T7 RNA polymerase (Promega) in the presence of Biotin RNA labeling mix (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The irrelevant control gene luciferase (dsLuc) was amplified from the luciferase T7 control plasmid (Promega). Female mosquitoes were injected intrathoracically (Ramirez et al. ...
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bioRxiv - Immunology 2020Quote: ... Transient transfection with plasmids was performed using standard manufacturer protocols with Fugene 6 (Promega, #E2691).
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bioRxiv - Genomics 2021Quote: All the reporter constructs were cloned into pGL-3 promoter plasmids (Promega, Madison, WI, USA). Fragments of the candidate RSCNEs were cloned into pGL3-promoter vector digested by BamH I and Sal □ downstream of the luciferase gene ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... DLD-1 cells were transfected with the guide and donor plasmids using Viafect (#E4981, Promega) reagent ...
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bioRxiv - Biophysics 2020Quote: ... This plasmid was used as a template for PCR (Pfu DNA polymerase; Promega, Madison, WI) using megaprimers consisting of 30 to 45-nucleotide homology flanking the altered nucleotides ...
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bioRxiv - Microbiology 2022Quote: U2OS cells (40,000 cells/well) were reverse transfected with FlavER plasmid using Fugene HD (Promega) in an 8-well chamber slide (Celltreat) ...
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bioRxiv - Cell Biology 2022Quote: Transcription of pGEM plasmids in vitro was carried out according to the manufacturer’s instructions (Promega) using SP6 polymerase ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were purified using the Wizard Plus mini-prep DNA purification system (Promega, Madison, WI) and screened for WT- and I212F-mutated allele strands by dual digestion analysis using EcoRI digestion (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 0.5 μg AsCpf1 or SpCas9 plasmid using Fugene 6 transfection reagent (Promega) according to manufacturer’s guideline ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli strains were performed using the PureYield™ Plasmid Miniprep System (Promega Corp., Madison, WI) according to manufacturer protocols ...
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bioRxiv - Immunology 2023Quote: ... was inserted upstream of the luc2 gene in the pGL4.10[luc2] plasmid (Promega Cat. E6651). As the SNP was located within the promoter region ...
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bioRxiv - Immunology 2023Quote: ... was inserted upstream of the luc2 gene in the pGL4.10[luc2] plasmid (Promega Cat. E6651). Following this step ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with a total of 4.5 µg plasmid DNA using Fugene HD (Promega): 0.25µg envelope vector pMD2.G (RRID:Addgene_12259) ...
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bioRxiv - Neuroscience 2023Quote: ... Both HEK293T cells and N2A cells were transfected with indicated plasmids via Fugene (Promega, E2311) as previously described46 ...
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bioRxiv - Neuroscience 2023Quote: ... plus helper plasmids pVSV-G (18 mg) and psPAX2 (27 mg) using FuGENE HD (Promega). DNA was incubated with 210 ml FuGENE HD in 4.5 ml Opti-MEM (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid-based donor repair templates for CRISPR were prepared in pGEM-T Easy vector (Promega). Homology arms (each around 800-1000 bp ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids pKHNH6 and pKHNH3 were linearised prior to transformation using restriction enzymes EcoRV (Promega, USA) and KpnI (Fisher ...
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bioRxiv - Physiology 2023Quote: Expression plasmids for luciferase and FincoR were mixed with a Coupled Reticulocyte Lysate System (Promega). After incubating at 30°C for 60 min ...
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bioRxiv - Plant Biology 2023Quote: High concentration (1 µg/µL) plasmid DNA was extracted via maxi-prep (Promega SV Wizard). Lower quality plasmid DNA (e.g ...
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bioRxiv - Neuroscience 2023Quote: Plasmid constructs were acutely transfected into HEK293 cells using Viafect reagent (E4981; Promega, Madison, WI), following the manufacture’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products of nifH and rnpB were each cloned into the pGEM-T plasmid (Promega). cDNA concentrations in nanograms per microliter were measured by Qubit 2.0 fluorometer (invitogen) ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... MCF-10A cells were transfected with the plasmids with the use of ViaFect (Promega, E4982). GFP-expressing cells were sorted into single-cell clones by flow cytometry with a Moflo Astrios EQ instrument (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transfected with 2 µg of plasmids with FuGene HD transfection reagent (Promega #E2312) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Purified 5 μg of pAd/CMV/V5-DEST plasmid was transfected using Fugene HD (Promega) when the 293A cells were 80% confluent ...
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bioRxiv - Developmental Biology 2024Quote: ... Kanamycin-resistant colonies were selected and cultured o/n purified with Plasmid Miniprep system (Promega) and verified by sequencing (Microsynth ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmids were extracted using Wizard® Plus SV Minipreps DNA Purification System Kit from Promega as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... and Alexa Fluor 680 goat anti-mouse antibody (Promega, W402B). Blots were imaged on a Peqlab Fusion SL Vilber Lourmat ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and mouse anti-βgal 1:500 (Cappel, Promega, Abcam), mouse anti acetylated tubulin 1:100 (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... from Santa Cruz Biotechnology, mouse anti-β-Galactosidase (Z378A, 1:500) from Promega, mouse anti-MYC tag (#2276 ...
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bioRxiv - Immunology 2019Quote: ... HRP-conjugated goat anti-mouse IgG (Promega, Madison, WI, USA) were added into each well ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-mouse IgG (Promega, WI; 1:3000-5000), HRP-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (Promega) were used at dilution 1:10.000 in PBS-T supplemented with 5% instant nonfat dry milk for 1 hour at RT ...
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bioRxiv - Microbiology 2021Quote: ... or anti-mouse IgG-HRP conjugate (1:10,000; Promega W402B) by incubating membranes for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... β III tubulin (anti-mouse, dilution 1:20,000, Promega, Madison, WI), and NR2B (anti-rabbit ...
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bioRxiv - Microbiology 2022Quote: ... As secondary antibodies α-mouse IgG HRP conjugate (Promega W4021) or α-rabbit IgG HRP conjugate (Cell Signaling #7074 ...
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bioRxiv - Cancer Biology 2022Quote: ... following intraperitoneal injection of luciferin substrate (3mg/mouse; P1043 Promega). Leukemia burden in the peripheral blood was monitored twice weekly by flow cytometric analysis of hCD45+ proportions ...