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Citations for Promega :
951 - 1000 of 5086 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and 500 ng of each of the packaging plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells in a 6-cm dish were transiently transfected with 1 μg of pGloSensor-22F cAMP plasmid (Promega, USA) and 1 μg of human wild-type or mutated A2AR or A2BR overexpression plasmid using polyethyleneglycol (6 μl ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at a density of 1*10^6 per 10cm plate and transfected on the following day using Fugene HD (Promega, Madison ...
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Biochemistry 2023Quote: ... 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with packaging vectors pMD2.G and psPAX2 along with relevant shRNA using Fugene 6 transfection reagent (Promega). Viral particles were harvested after 48 hours in DEMEM/ 40% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Genetics 2023Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Promega) at 37 °C for 16 h ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... by transfection of the pSpCas9(BB)-2A-Puro (PX459) plasmid containing A3B gRNAs with FuGENE 6 Transfection Reagent (E2691; Promega). 16h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... 6 or 24 hours as indicated in the respective figure legends before lysis using the HiBiT lytic assay buffer (Promega) per manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Guides targeting 5’ and 3’ regions (see primer sequences in Supplementary File 6) of the PIP5Pase coding sequence were produced by in vitro transcription with T7 polymerase (Promega), and co-transfected with recombinant saCas9 Nuclease NLS Protein (Applied Biological Materials Inc.) ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown to ∼70% confluence in 6-well plates and co-transfected with a gRNA to TBC1D23 (12) and pmaxGFP using Fugene6 (Promega) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... mammalian expression vectors containing the fusion gene under the control of a weak L30 promoter and containing a Neomycin resistance marker were transfected into U2OS cells at 70% confluence using FuGENE 6 (Cat. No. E2691, Promega). Transfected cells were selected with G418 (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... and RSPO1-CM were produced as described (Fenderico et al., 2019; Tauriello et al., 2010) Transfections were performed using either FuGENE 6 (Promega) according to manufacturer’s protocol for β-catenin-mediated reporter assays and microscopy or polyethylenimine (PEI ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... plated in 6-well dishes were centrifuged at 1000xg for 10 minutes and suspended in 5x Passive Lysis buffer (Promega). Each data point represents an individual well ...
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bioRxiv - Molecular Biology 2024Quote: About 5 μg empty pcDNA3 vector or ZNF410 full-length and ZNF410-ZF plasmids was transiently transfected with FuGENE 6 (Promega) into 10 cm plates of COS-7 cells ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins in the excised band were digested with Trypsin (Promega, V5280). The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used to perform in vitro translation assay ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were digested with 10ng/ul of sequencing grade trypsin (Promega) overnight at 37C ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then digested using modified trypsin (Promega, P/N V5113) at a 1:20 (w/w ...
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bioRxiv - Immunology 2019Quote: ... Proteins were digested with sequencing grade trypsin (1:100; Promega, USA) overnight at 37°C and peptides concentration was checked by spectrophotometry (Nanodrop 2000 ...
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bioRxiv - Microbiology 2021Quote: ... Crosslinked proteins were digested by the addition of 1μg trypsin (Promega) and overnight incubation at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... The protein digestion was performed by adding 0.2 µg LysC (Promega) in 50 µL of 100 mM ABC for 3h at 1200 rpm at 37°C followed by overnight trypsin digestion using 0.2 µg of trypsin (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Fusion proteins were expressed by TnT® Quick Coupled system (Promega). 15 μL of HA tagged protein was then incubated with candidate GST-coated beads at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were digested by addition of sequencing-grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with 500 ng trypsin (Promega, Madison, USA) overnight at 37°C on a shaking incubator ...
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bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were on-bead digested with 1 µg trypsin (Promega) for 4 hours at 37 °C under agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Then the protein was digested with sequencing grade modified trypsin (Promega) (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 12.5 ng/uL modified trypsin (Promega) in 50 μL 100 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... anti-GFP (Abkine A02020) and Magnetic Protein G Beads (Promega, G747A) overnight at 4°C with gentle agitation ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were digested with mass spec grade Trypsin/LysC mix (Promega) at a 1:50 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were mixed with Trypsin Gold (Promega, Madison, WI, USA) at a 50:1 ratio (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin overnight (0.12 μg Trypsin Gold [Promega UK Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... Enzymatic digestion of proteins using LysC/Trypsin Mix (Promega, Fitchburg, WI) was then performed according to the technical manual ...
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bioRxiv - Plant Biology 2021Quote: ... and tryptic protein digestion (1:100 enzyme:protein, V5111, Promega, Madison, USA) was performed using Microcon-30 kDa filter units (Millipore ...
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bioRxiv - Physiology 2021Quote: ... and overnight protein digestion using 4 μg MS-grade trypsin (Promega) at 37°C ...
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bioRxiv - Biochemistry 2020Quote: The protein samples were digested with trypsin (Promega, mass spectrometry grade) in (NH4)HCO3 buffer (0.1 M ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then digested with 12.5 ng/µL modified trypsin (Promega) in 50 µL 100 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: Bead-bound protein was digested with 100 ng trypsin (Promega, V5113) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested with sequencing-grade modified trypsin (Promega, Madison, WI) overnight at 37 ºC ...
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bioRxiv - Microbiology 2019Quote: ... Other samples were processed using protein precipitation solution (A795A; Promega, France) [12] ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then in-gel digested using trypsin (sequencing grade, Promega). Resulting peptides were analyzed by online nanoLC-MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus or HF ...
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bioRxiv - Systems Biology 2019Quote: ... protein was digested in 1.65 µg of sequencing-grade trypsin (Promega) for 6 hours at 37°C ...
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bioRxiv - Genetics 2020Quote: ... 40 μg of extracted proteins were fragmented by trypsin digestion (Promega Sequencing Grade Modified Trypsin V5111) ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were digested overnight with trypsin (1:50) (Promega, USA) in 1% (w/v) ...