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Citations for Promega :
1051 - 1100 of 5086 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Cell Biology 2019Quote: ... a puromycin resistance marker and specific gRNA guides against VPS35, SNX5 or SNX6 (Kvainickas et al., 2017; Simonetti et al., 2017) using FuGENE® 6 (Promega). The day after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Cancer Biology 2020Quote: ... used for infection of p53-null NSCs were obtained from previously transfected DF1 chicken fibroblasts (ATCC #CRL-12203) using FuGENE 6 Transfection reagent (Promega #E2691), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... the cells were transiently transfected with pUC57 constructs harboring 1.3-mer HBV genomes (genotypes A2, B2, C2, and D3) using FuGene 6 transfection reagent according to the manufacturer’s instructions (Promega, WI, USA). The generation of plasmids has been previously described and this transient expression system relies on the endogenous promoters of HBV for transcription (6) ...
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bioRxiv - Molecular Biology 2019Quote: ... Transient transfections were performed in 24-well plates using 500ng of dCas9 expression vector and 250ng of equimolar pooled or individual sgRNA expression vectors mixed with FuGENE® 6 (Promega) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: XAB2-GFP and RNAP2-GFP stably expressing cell lines were produced by transfecting XAB2-GFP or RNAP2-GFP using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’ protocol ...
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bioRxiv - Immunology 2021Quote: Pseudoviruses expressing complete SARS-CoV2 spike genes were prepared by transient transfection of HEK293T cells with three plasmids: SARS-CoV2 MLV-gag/pol and MLV-CMV-luciferase plasmids using Fugene 6 (Promega Inc.) as described earlier (Rogers et al. ...
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bioRxiv - Microbiology 2022Quote: ... The effector cells and serum were incubated with the cells for 6 hours after which 75ul Bio-Glo reagent (Promega; G7940) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... transfection was carried out by adding 2 μg of freshly prepared bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311), following the instructions provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... a targeting vector was constructed by subcloning a 9.1 kb region from an Akr1b7 positively identified C57BL/6 fosmid clone (WI1-1577M19) into the 2.45 kb-iTL cloning vector derived from the pSP72 vector (Promega; Madison, WI). The region was designed so the long homology arm (LA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
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bioRxiv - Microbiology 2024Quote: ... and simultaneously transfected with 0.4 μg of respective EGFP-Rab plasmids using FuGENE 6 transfection reagent (Cat. E269A, Promega, Madison, WI) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Cell Biology 2020Quote: ... Halotag fusion proteins were labelled with Janelia Fluor 646 Halotag Ligand (Promega) for live cell imaging ...
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were in-gel digested with trypsin (Promega, Madison, WI, USA), and the resulting peptide mixtures were processed on StageTips (Rappsilber ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 μg of protein was digested with sequencing grade trypsin (Promega) at 1:50 trypsin:sample protein amount for 14 hrs on a ThermoMixer at 37°C and 600 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant protein was then digested overnight with sequencing grade trypsin (Promega). The trypsin ...
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bioRxiv - Plant Biology 2019Quote: ... Chemiluminescent protein detections were conducted with ECL Western Blotting Substrate (Promega, W1001), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested overnight at 37°C with 800 ng trypsin (Promega). Digestion was stopped by adding 5 μl TFA (Trifluoroacetic acid ...
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bioRxiv - Cell Biology 2019Quote: ... Trypsinzation was achieved by incubating eluted protein with excess trypsin (Promega V5111) at 37°C overnight (16-18 hours) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then subjected to digestion with trypsin (Promega; Madison, WI, USA) overnight at 37°C at 50:1 protein:trypsin ratio ...
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bioRxiv - Neuroscience 2021Quote: ... the isolated proteins were digested overnight with sequencing grade modified trypsin (Promega). The resulting peptides were resolved on a nano-capillary reverse phase column (PicoFrit column ...
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bioRxiv - Plant Biology 2021Quote: ... TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega L3260), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were in gel-digested overnight with sequencing grade trypsin (Promega). The resulting tryptic peptides were desalted using in-house C18 disks ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was performed by overnight digestion with trypsin sequencing grade (Promega).
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with 500 ng of sequencing-grade modified trypsin (Promega), and the resulting peptides were resolved on a nano-capillary reverse phase column ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested with 0.2 µg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate at 37°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Physiology 2022Quote: ... and proteins therein were in-gel digested with trypsin (Promega, Dübendorf, Switzerland), and resulting peptide mixtures were processed on STAGE tips and analyzed by LC-MS/MS.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Plant Biology 2021Quote: ... Total protein was extracted using 100 μL of Passive Lysis Buffer (Promega). 20 μl of the supernatant were used to measure the LUC and RLUC activity using a Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2020Quote: ... followed by protein digestion with sequencing grade modified trypsin (Promega, cat. V5111) at a protein-to-enzyme ratio of 50:1 at 37℃ overnight ...
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bioRxiv - Microbiology 2022Quote: ... 300 µg of protein per sample were treated with Trypsin Gold (Promega) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA) (enzyme-to-protein ratio of 1:50 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was further analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... together with molecular weight marker (Broad range molecular weight protein markers, Promega) was added to a 15% SDS-PAGE gel and separated under 200 volts ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA) (enzyme-to-protein ratio of 1:50 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was extracted using a passive lysis buffer (Cat# E1910, Promega). LUC activity was measured using a GloMax®20/20 Luminometer (Cat# E5311 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were then digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA) as described previously (Khusainov et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Proteins were reduced and digested with sequence-grade modified Trypsin Gold (Promega). Peptides were eluted with ammonium bicarbonate and acidified peptide mixtures from each biological replicate were analyzed in technical triplicates by nanoflow reverse-phase liquid chromatography tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...