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Citations for Promega :
801 - 850 of 5086 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) was added for overnight digestion at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested with endoproteinase-LysC (Promega, 1:100, w:w) for 4 h at 37 °C and then with trypsin (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Systems Biology 2023Quote: ... Protein was digested with Trypsin Gold (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio overnight at 37 °C with agitation at 1000 rpm.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
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bioRxiv - Microbiology 2023Quote: ... the protein suspension was digested with 4 μg trypsin (Promega) in 40 μL NH4HCO3 (50 mM ...
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bioRxiv - Systems Biology 2023Quote: ... and catalytic hydrolysis of proteins was initiated by trypsin (Promega) at a weight ratio of 1:50 protease:substrate ...
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bioRxiv - Systems Biology 2023Quote: ... and catalytic hydrolysis of proteins was initiated by trypsin (Promega) at a weight ratio of 1:50 protease:substrate ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were digested using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
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bioRxiv - Microbiology 2023Quote: ... Protein digestion was achieved with 2.5 µg of trypsin (Promega) and overnight incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: The protein was digested overnight with 0.1 μg LysC (Promega) and 0.8 μg trypsin (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested with trypsin (Promega; 1:100 (w/w) enzyme-to-protein ratio ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were digested by trypsin (Promega, Madison, WI, USA), and peptides were extracted from the gel with an acetonitrile/formic acid solution ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were enzymatically digested with 0.5 ug Trypsin Gold (Promega) for 16 h ...
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bioRxiv - Genomics 2023Quote: ... Proteins were digested with modified trypsin (sequencing grade, porcine, Promega) in enzyme:protein ratio of 1:50 overnight at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... proteins in the plugs were digested overnight with trypsin (Promega) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were digested overnight with sequencing-grade modified trypsin (Promega). Then ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Immunology 2023Quote: ... Proteins were cleaved overnight with 1 µg of trypsin (Promega) at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve ...
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bioRxiv - Plant Biology 2023Quote: ... alkylation (55 mM CAA) and protein digestion (trypsin-gold, Promega) the resulting peptides were purified using self-packed StageTips (C18 material ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in 50 mM TEAB containing trypsin (Promega) at a 1:20 enzyme-to-protein ratio overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested with 1µg trypsin (Trypsin Gold, Promega Corporation) overnight at 37°C in a shaker at 1250 rpm ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of 7.5×104 Jurkat effector cells expressing either human FcγRIIIa or murine FcγRIV (Promega) resuspended in assay buffer were added to each well ...
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bioRxiv - Molecular Biology 2021Quote: Full-length human RNU6 sequence (110 nucleotides) was amplified by PCR and cloned into psiCHECK2 (Promega), downstream of Renilla luciferase ...
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bioRxiv - Cancer Biology 2020Quote: ... Standards for quantification were created by serial dilution of pre-quantified BSC human male DNA (Promega). Amplification reactions were performed in duplicate using 96 well-plates using a CFX96 Touch Real-time PCR Detection System (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Human and murine NLRC5 and CXCL10 promoters were cloned into pGL3 basic luciferase reporter vector (Promega). All mutated plasmids were constructed from wild type plasmids by Fast Mutagenesis System (TransGen Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Immunology 2022Quote: Replication competent HIV were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 3 ug of pCAG-NLS-Bxb1 was diluted in 250 uL of OptiMEM and 6 uL of Fugene (Promega). On day 2 ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for an additional 6 hours at 37°C before 75μl of Bio-Glo Luciferase Assay Reagent (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... All the clones were transfected into the HEK-293T cells at 90% confluency using FuGene 6 as transfection reagent according to the manufacturer’s instructions (Promega). dsiRNAs targeting Rpp30 (10 nM ...
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μg of sgRNA construct and 1.5 μg of linearized HDR plasmid were transfected into RPE1 cells using Fugene 6 (Promega). Positive cells were FACS sorted to isolate the Ndc80-EGFP expressing cells and single clones were identified by visual inspection with fluorescent microscope ...
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bioRxiv - Biochemistry 2019Quote: ... 70 000-100 000 cells (in 200-450 μl DMEM) were seeded into each well containing a transfection cocktail of 0.4 μl of Fugene 6 (E2693, Promega), with 20 μl Opti-MEM (11058-021 ...
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bioRxiv - Developmental Biology 2019Quote: ... were injected with dsRNA throughout and frozen in Trizol on dry ice after 6 days in vitro for RNA extraction according to manufacturer’s protocol and DNAse (Promega) treatment for 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... were transfected with 1 µg of DNA and 4 µL FuGENE 6 in 100 µL Opti-MEM media for 24-36 hours according to the manufacturer’s instructions (Promega). Cells were fixed with 4% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1 cells were co-transfected with 1 µg KIF1C-mCherry-FRB and 0.5 µg FKBP-GFP-Myc-MAO using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with WT or mutant C10 constructs using the FuGENE 6 transfection reagent (Promega, cat# PRE2691). The cells were incubated at 37 C at least overnight before downstream analysis.