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Citations for Promega :
51 - 100 of 1618 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Transduced cells were incubated for 24 hours at 37°C/5% CO2 and luminescence measured by addition of 40μl of ONE-Glo reagent (Promega) with detection using a Tecan Spark plate reader ...
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bioRxiv - Cancer Biology 2021Quote: ... After 24 hours the Dual-Glo Luciferase Assay System (Promega) was used and luminescence was measured by the Synergy HT microplate reader (Biotek).
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bioRxiv - Neuroscience 2023Quote: ... 24 h later cells were transfected with Fugene HD (Promega) and constructs containing dCas9-VPR and gRNA vectors targeting the conserved DAR region (500ng plasmid DNA in molar ratios sgRNA:dCas9-VPR) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were authenticated using the GenePrint 24 kit (Promega) and DSMZ Online STR Analysis database at the DNA Sequencing Core at the University of Utah ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed 24 hpi in Passive Lysis Buffer (Promega), and NanoLuc luciferase reporter gene expression was detected using a Nano-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µL of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent (#G3582; Promega, Madison, WI) was added and incubated at 37°C for 45 mins ...
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bioRxiv - Immunology 2022Quote: ... DDX60 (Fw 5’- AAGGTGTTCCTTGATGATCTCC-3’ Rv : 5’ -TGACAATGGGAGTTGATATTCC-3’) as analyzed by semiquantitative PCR using the SYBR Green assay GoTaq® qPCR Master Mix (Promega) with standardized primers (Metabion) ...
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... cells were co-transfected with RIG-I-2CARD (5 ng) and lysed at 24 hours after transfection using Passive Lysis Buffer (Promega). Samples were processed and luciferase activity was measured using the Dual-Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... 1.0 μM 431R2 [5’-CTCTTCACAACAGTCATGTGCG-3’] and 1.0 U GoTaq2 polymerase (Promega). Cycling conditions were 30 s at 98°C ...
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bioRxiv - Microbiology 2022Quote: ... 24 h later by adding 1 μM of Coelenterazine-H (Promega) at 1:400 dilution in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed 20-24 hpt in Renilla lysis buffer (Promega) for 45 min at room temperature under steady shaking (650 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed 20-24 hpt in Renilla lysis buffer (Promega) for 45 min at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... The kit used for this was the GenePrint 24 system (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed 20-24 hpt in Renilla lysis buffer (Promega) for 45 min at room temperature under steady shaking ...
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bioRxiv - Biochemistry 2023Quote: ... MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (#G3581) was purchased from Promega (Charbonnières-les-Bains, France).
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bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...
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bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
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bioRxiv - Developmental Biology 2022Quote: ... and 175 μg/ml 5-bromo-4-chloro-3- indolyl-phosphate (BCIP) (Promega). Reactions were stopped as the signal became apparent with three PBS rinses ...
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bioRxiv - Microbiology 2020Quote: ... 24 hours post-transfection cells were lysed with passive lysis buffer (Promega) and luciferase bio-luminescent signals were read on a FLUOstar Omega plate reader (BMG Labtech ...
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bioRxiv - Microbiology 2020Quote: ... 24 hours post-transfection cells were lysed in passive lysis buffer (Promega) and luciferase bio-luminescent signals were read on a FLUOstar Omega plate reader (BMG Labtech ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of plasmids was done 24 hours after plating using FuGENE6 (Promega) at a DNA/FuGENE ratio of 1:4.5 according to manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free using a commercial testing kit every three months.
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bioRxiv - Cancer Biology 2022Quote: ... verified for cell type identity using the GenePrint 24 system (Promega, B1870), and confirmed as mycoplasma-free every three months using a commercial testing kit ...
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bioRxiv - Physiology 2022Quote: ... were transfected in a 24-well plate using Fugene HD (Promega, E2311) at 30% confluency ...
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bioRxiv - Biochemistry 2021Quote: ... Luminescence was quantified 24 hours after treatment using the DualGlo kit (Promega) on a BioTek Neo plate reader ...
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bioRxiv - Microbiology 2022Quote: ... 24 h post transfection the Dual-Luciferase® Reporter Assay System (Promega) was used according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... Luminescence was measured 24-36 hours after transfection using Nano-glo (Promega) and viability (that was not affected ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed 24 hours after transfection in passive lysis buffer (Promega) and firefly and Renilla luciferase activity were measured ...
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bioRxiv - Biophysics 2024Quote: ... After 6 to 24 hours cells were transfected using Viafect reagent (Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C in 5% CO2 for 24 hours and luciferase activity was measured using a ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... with an added 5’-CACC-3’ at 5’-end and 5’-AAAC-3’ at 5’-end of the complementary strand were annealed in 10xT4 ligation buffer (Promega M1801) by continuous cooling from 95°C to 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Caspase-Glo 3/7 Reagent (Caspase-Glo® 3/7 Assay, Promega) was added to the wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was detected using Caspase-Glo 3/7 (Promega). Annexin V/Propidium Iodide staining was performed using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... caspase-3/7 levels were examined using Caspase-Glo 3/7 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... or 3) Pepsin (Promega). 1 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL RNasin (Promega) and 100 µL lysis buffer for a total volume of 300 µL for IP by rotation for 2 hours at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... LgBiT and linker (5’-GGGAGTTCCGGTGGTGGCGGGAGCGGAGGTGGAGGCTCGAGCGGT-3’) inserted into the HaloTag-removed-pFC15A vector (Promega). GPCR sequences were obtained from PRESTO-tango kit (Addgene Kit #1000000068) ...
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bioRxiv - Microbiology 2020Quote: ... 24 hours later cells were lysed in 100μl of Renilla lysis buffer (Promega) and Gaussia activity was measured using a Renilla luciferase kit (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The enhancer constructs were transfected 24 h after seeding using FuGENE6 (Promega, US) as the transfection reagent ...
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bioRxiv - Biophysics 2021Quote: ... Cells were subsequently transfected 24-48h before imaging using FuGENE6 transfection reagent (Promega) and plasmid DNA ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were collected 24 hours post-transfection using Passive lysis buffer (Promega). Samples from three technical replicate transfections were measured in duplicate using a Zylux FB12 luminometer and the Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were seeded in 24-well plates and transfected with FuGENE (Promega) and stained as described in the immunofluorescence section.
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bioRxiv - Developmental Biology 2021Quote: ... in 24-wells plates (700000 cells/well) using FuGene HD transfection Reagent (Promega). Experiments were repeated timely independently at least 3 times ...
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bioRxiv - Systems Biology 2023Quote: ... TX)33 24 hours before analysis using CellTiter-Glo viability assay (Promega, Madison, WI). Response was normalized to a DMSO control.
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bioRxiv - Microbiology 2023Quote: ... The cells were incubated for 20-24 hours after which ONE-GloEX (Promega) was added to each well and the plates were incubated for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Luciferase expression was analyzed after 24 hours per manufacturer instructions (Promega, Madison, WI). Cells were washed with sterile PBS and 20 μL of 1x lysis buffer was added to each well ...