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Citations for Promega :
451 - 500 of 1618 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells were collected 24-hr post transfection and firefly and renilla luciferase were analyzed with the Dual Luciferase Reporter Assay system (Promega). Normalization was performed using Western blot analysis for protein concentration ...
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bioRxiv - Microbiology 2024Quote: ... cells were infected at MOI 1 for 24 h and cells were frozen dry at -80°C for 30 min and lysed in Passive Lysis Buffer (Promega). Bioluminescence was measured using the Nano-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Physiology 2024Quote: ... Proliferation rate of NIH-3T3 fibroblasts was determined 24 h after MNP treatment via MultiTox-Fluor Multiplex Cytotoxicity Assay (Promega). As controls ...
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bioRxiv - Microbiology 2023Quote: ... transfected cells were treated with human IFN-γ at (100 U/mL for 24 hr) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on SpectraMax iD3 (Molecular Devices ...
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bioRxiv - Cell Biology 2023Quote: ... and bacterial bioluminescence was measured in relative luminescence units (RLUs) every 24 h for up to seven days using a GloMax Multi Detection System (Promega) (35) ...
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bioRxiv - Cell Biology 2023Quote: ... 24 h later the cells were incubated with a final concentration of 16 nM Halo-Ligand JF 549 (Promega, GA1110) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 24 h after transfection and luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Genomics 2023Quote: ... Cells were harvested 24 hours after transfection and luciferase measured using Luciferase Assay System and the GloMax Multi-Detection System (Promega). For Verteporfin (VP ...
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bioRxiv - Immunology 2022Quote: 1×105 721.221 or 293T cells were plated in 24 well plates and transfected with 400 ng of pGL3 and 100 ng of pRL-CMV (Promega). Two days later ...
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bioRxiv - Cell Biology 2022Quote: ... Luciferase activity was detected 24 h after transfection and normalized to Renilla luciferase activity using the Dual-Glo Luciferase system (Promega). The relative luciferase unit was defined as the ratio of Firefly versus Renilla ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were analyzed 24 h after transfection to measure firefly and renilla luciferase activities using GloMax 96 microplate luminometer (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... For ACE2 overexpression HEK293T cells were transfected for 24 h with human ACE2 (pCG1-hACE2, 200 ng DNA/well) using JetPrime (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 24 h after transfection and luciferase activity was measured with Dual-Luciferase Reporter Assay (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...
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bioRxiv - Cancer Biology 2023Quote: ... Firefly and Renilla signals were measured 24 h after transfection using the Dual-Glo Luciferase Assay System (Promega, cat#E2920) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated overnight with 2000 U/mL of universal Type I IFN (PBL Bioscience) and lysed at 24 h post treatment using Passive Lysis Buffer (Promega). Samples were processed and luciferase activity was measured using the Dual-Luciferase Assay System (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... HBEC cells were incubated for an additional 24 hours and then luciferase activity was measured using the Dual-Luciferase Reporter assay kit (Promega) on a Glomax instrument (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... and the viability was measured 24 or 48 h after compound treatment using CellTiter-Glo luminescent cell viability assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Luciferase measurements in the supernatants were conducted at 24 hours post-infection using 50 μL of supernatant and 50 μL of nano-Glo luciferase reagent (Promega).
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bioRxiv - Bioengineering 2024Quote: ... The total amount of extracted DNA was quantified using UV-Vis and then analyzed using the Geneprint 24 system (Promega) for its Short Tandem Repeat profile ...
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bioRxiv - Microbiology 2024Quote: ... together with either 20 ng dsRNA targeting FFLuc (dsFFLuc) or 20 ng dsRNA control (dseGFP) and lysed at 24 hpt in 1X PLB (Promega). To determine FFLuc/RLuc levels ...
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bioRxiv - Neuroscience 2024Quote: ... The luciferase assays were performed 24 h after transfection using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega, N1551) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The transfection was terminated 24 h later by removing the media and lysing the cells using 25 µl 1x Passive Lysis Buffer (Promega). For eIF1/eIF5 knockdown experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... The proliferation curve was built by harvesting one plate every 24 hours and performing the Cell Titer Glo (Promega, G7572) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with ferroptosis inducers and inhibitors for 24 hours and cytotoxicity was measured using Cell Titer Glo2 Assay (Promega) based on the manufacterer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cell media of MDMi treated with WFSE or DEP was harvested after 24 hr and levels of nitrite were determined using the Griess reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-R: 5′-TGC CTC GAG CTC AAG TGT CTG TGGATC AC-3′) into pGEM T-easy vector (Promega, Madison, WI, USA). A standard curve was generated by determining the copy numbers derived from serial dilutions of the plasmid (103–109 copies) ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were developed with a commercial solution of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) according to the manufacturer (Promega, Madison, WI), in alkaline developing solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability was measured 3 days later using CellTiterGlo (Promega).
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bioRxiv - Microbiology 2021Quote: ... in a 3:1 ratio of FuGENE®HD (Promega) transfection reagent to DNA ...
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bioRxiv - Immunology 2020Quote: ... the protein suspension was digested with 3 μg trypsin (Promega) in 40 μl 25 mM NH4HCO3 overnight at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... (3) we used ProNex Size-Selection DNA purification System (Promega) for purification of PCR product ...
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bioRxiv - Cancer Biology 2020Quote: ... Apoptosis was assessed with Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s instruction.
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bioRxiv - Genetics 2021Quote: ... and Caspase-Glo® 3/7 Assay System (Promega, G8090), respectively ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... followed by 3 h at 37°C using trypsin (Promega). All proteolytic digests were acidified to pH 2 by addition of 10% formic acid and directly analyzed by LC-ESI-MS/MS ...
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bioRxiv - Cell Biology 2023Quote: ... 2-3 µg bacmid DNA was transfected (FuGene HD, Promega) into 2 ml Sf9 cells plated at 0.5x106 cells/ml density in a 6-well plate and left to incubate for 5-7 days 27ºC without shaking ...
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bioRxiv - Neuroscience 2022Quote: ... in combination with Caspase-Glo® 3/7 Reagent (Promega) [33] ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... we utilized BetaFluor β-gal assay kit (Promega 70979-3) to distinguish between Kif17+/- and Kif17-/- littermates at postnatal day 8 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The caspase 3/7 Glo assay (Promega, Madison, WI, USA) was performed on the white plates according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the permeabilization protocol used a 3 minute 0,02% digitonin (Promega) exposure ...
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bioRxiv - Cell Biology 2022Quote: ... and caspase-3/7 assay (Cat # G8090, Promega, Madison, WI) were performed using commercially available kits as per the instructions to assess proliferation and apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: ... after which 3 μL of CellTiter-Glo reagent (Promega, G7572) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... Tissues were embedded in 3% low melting point agar (Promega). Formalin embedding ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was measured by luminescent caspase 3/7 assay (Promega). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl of nuclease free water (Promega, Cat No. P119E), and 5 µl of cDNA using the following program ...
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bioRxiv - Cancer Biology 2024Quote: ... and Caspase 3/7 activity by Caspase-Glo (#G8093, Promega). Plates were read on a SpectraMax I3 plate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Caspase-Glo® 3/7 Assay kit (Promega), following the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... with the Caspase-Glo 3/7 Assay System (Promega, G8090). Briefly ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... The absorbance of cells was measured every 24 hours using CellTiter 96® Non-Radioactive Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Firefly and Renilla luciferase activities were determined 24 hours post transfection with the dual-luciferase reporter assay system (Promega, cat.no. E1910). Firefly luciferase readings were normalized against internal control Renilla luciferase and calculated as fold differences against the activity obtained from cells transfected with empty vector.