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Citations for Promega :
901 - 950 of 2430 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Microbiology 2019Quote: ... 500 nM of each primer (5’-TCCTGCTCAACTTCCTGTCGAG-3’ and 5’-CACAGGTCAAACCTCCTAGGAATG-3’) and 0.1 µL hot-start Taq DNA polymerase (Promega) in 20 mM Tris−HCl pH 8.3 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and cDNA synthesis was carried out using an oligo d(T)15 primer and MMLV reverse transcriptase (Promega, USA) enzyme ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Cell Biology 2019Quote: ... 500 ng RNA was used for reverse transcription using random primers and the GoScript master mix according to the manufacturer’s instructions (Promega). Quantitative real-time PCR was used to examine the expression levels of TRAP1 and HIF1A with GAPDH as the reference gene.
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bioRxiv - Molecular Biology 2019Quote: ... RNA from splicing reactions (∼20 fmol pre-mRNA in 10.5 µL splicing reaction) was analyzed by primer extension (15 µL reaction volume total) using AMV reverse transcriptase (Promega) as previously described (Mayas et al ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of forward and reverse primer (5 μM, see Supplementary Table 1) and 5.5 μL of Nuclease-free Water (Promega). The PCR I was performed as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription was performed on total RNA using the GoScript Reverse Transcription System with an oligo-dT primer (Promega). Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... 10 ng of RNA was used with oligo (dT) primers and AMV reverse transcriptase and reverse transcription was performed according to the manufacturer’s instructions (Promega). Using a GeneAmp 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h at 37 °C and cDNA was then synthesized using a Moloney murine leukemia virus (MMLV) reverse transcription kit with random primers following the manufactureŕs protocol (Promega). To check for the presence of remaining DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... 1μg of mRNA was reverse-transcribed using oligo-dT-VN primers and ImProm-II as the reverse transcriptase (Promega) in triplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 400 ng of RNA using ImProm-II Reverse Transcription system kit and random primers (Promega). Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 500 ng of RNA was added to each 11.8 ul reaction along with 0.5 ul of random primer (Promega C1181) and 1 ul of dNTP (Promega U1511) ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...
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bioRxiv - Immunology 2020Quote: ... was reverse transcribed from RNA extracts using GoScript reverse transcriptase with random primers according to the manufacturer’s instructions (Promega). qPCR reactions were carried out on a Stratagene Mx3005p thermal cycler (Agilent Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... LaMATE2ORF sequence was cloned in pGEM-T easy vector (for primers see Table S1, Promega Italia Srl, Milan, Italy) and deposited in the NCBI database (KY464927).
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed on total RNA using the GoScript Reverse Transcription System with an oligo-dT primer (Promega). Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vector using the manufacturer’s protocol (Promega; CAT #A1360). Vectors were transformed into E ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl (200 nM) of each primer and 10 μl of GoTaq® qPCR Mastermix 2X (Promega Corporation, USA). The amplification program consisted of ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized by reverse transcription using RevertedAid H minus reverse transcriptase (ThermoFischer Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3’ end of the MERS-CoV genome ...
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bioRxiv - Neuroscience 2021Quote: ... the extracted RNA was reverse-transcribed into cDNA with the oligo (dT)15 primer with GoScriptTM Reverse Transcriptase (Promega). In Fig ...
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bioRxiv - Molecular Biology 2022Quote: Edited loci were amplified using locus-specific primer pairs described in Supplementary Table 1 using GoTaq master mix (Promega) and 200ng of genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... [32P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random hexanucleotide-primer kit following the supplier’s instructions (Promega). As a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Inserts (primers and size are presented in Supplementary Table S5) were cloned into pGEM-T Easy (Promega, Madison, WI) vector and confirmed by restriction and sequencing analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Developmental Biology 2023Quote: ... A minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Cell Biology 2023Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the ProNex Size- Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... reverse transcription was carried out using 1 μg of total RNA and random primers (Reverse Transcription System, Promega, A3500). IQTM SYBR® Green qPCR Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out with oligo(dT) primers using Moloney murine leukemia virus reverse transcriptase according to the manufacturer’s instructions (Promega). The qPCR reaction was performed on an Applied Biosystems QuantStudio3 thermocycler using a SYBR™ Green PCR Master Mix (Thermo Fisher scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (1000ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript reverse transcriptase kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Plant Biology 2020Quote: ... The positive PCR products were cleaned using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI) and submitted for Sanger sequencing at Roy J ...
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bioRxiv - Genetics 2022Quote: Chromatin accessibility by real-time PCR (ChART-PCR) was performed as previously described (75) using 20 U DNase I (Promega). To assess nucleosome occupancy ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting cDNA was subjected to PCR or qPCR analysis using Phire Green Hot Start II PCR Master Mix (Promega) or GoTaq qPCR Master Mix (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Wisconsin, USA, A9281) and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes ...
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bioRxiv - Biochemistry 2021Quote: ... Both PCR products were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation [37] ...
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bioRxiv - Microbiology 2019Quote: ... Products of PCR reactions were purified using the Wizard® SV Gel and PCR Clean-Up system (Promega, Madison, USA). Sequencing reactions were performed using the Big-DyeTM Terminator Version 3.1 Ready Reaction Cycle Sequencing Kit (Applied BioSystems ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was amplified with Phusion HotStart II DNA polymerase and the PCR fragment was ligated with T4 ligase (Promega) and transformed into Novablue competent cells ...
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bioRxiv - Plant Biology 2020Quote: ... The overlapping PCR products were separated and purified by the Spin Column PCR Product Purification Kit (Wizard SV Gel and PCR Clean-Up System) following manufacturer’s instruction (Promega, WI). Then ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplicons were gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega Cat. No. A9281). Next ...
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bioRxiv - Genomics 2019Quote: ... PCR products run on 1% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up System (Promega). Purified PCR products were sequenced and analyzed with the Sequence Scanner Software 2 Version 2 (Applied Biosystems).
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA), quantified ...
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments containing the T7 promoter sequence were further amplified by PCR and purified using a Wizard SV Gel and PCR clean-up system (#A9281, Promega) to prepare templates for in vitro transcription ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed according to the method of Ehira and Miyazaki43 using Go Taq qPCR Master Mix (Promega). Primers used for PCR and qRT-PCR are listed in Supplementary Table 5.
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Synthetic Biology 2023Quote: ... while the purification of the PCR fragments was performed using the Wizard SV Gel and PCR Clean-Up System (Promega).
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bioRxiv - Plant Biology 2023Quote: ... PCR reactions were conducted in 10 μL volume comprising 5 μL Promega 2x PCR Master Mix (Promega, Madison, Wisconsin, USA), 10 pmol of forward and reverse primers ...
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bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega, catnr: V5111) at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Evolved strains were thawed and grown overnight in 150 ml of liquid medium and DNA was extracted using the Wizard Genomic DNA Purification Kit from Promega (WI, USA). One DNA sample (20 to 60 µg ...