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Citations for Promega :
801 - 850 of 2430 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The second PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega) and the Agencourt AMPure XP Kit (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the plasmid constructs (150 ng) were transfected into HEK 293T cells at their 90% confluency using Dual-Luciferase Reporter Assay System (Promega). The Luciferase activity was measured 72 h (48h for DsiRNA and 24 h for plasmid constructs ...
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bioRxiv - Cell Biology 2020Quote: ... Remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 150 ng trypsin (Trypsin Gold, Promega) at room temperature for 90 min ...
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bioRxiv - Plant Biology 2021Quote: ... ground in liquid nitrogen and total RNA from approximately 150 mg of ground leaf material was extracted with a ReliaPrep miRNA Cell and Tissue Miniprep System kit (Promega). A stem loop pulsed RT-PCR protocol [8 ...
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bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 150 ng trypsin (Trypsin Gold, Promega) for 90 min at room temperature in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were lysed by the addition of 150 μl of lysis solution (1.25 mg/ml lysozyme, 2.5 mg/ml BSA, 1X CCLR) (Promega, WI, USA) and incubating at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... After 48hr the cells were washed with PBS and lysed in a sonication bath in 150 ul of 1x Passive Lysis Buffer (Promega) supplemented with Protease Inhibitor cocktail (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... all mice were intraperitoneally injected (150 mg/kg) with a 30 mg/ml solution of D-luciferin (Vivo Glo Luciferin, P1043, Promega) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed in cell lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton-X with protease inhibitor cocktail (Promega)) and subjected to Western blotting ...
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bioRxiv - Neuroscience 2023Quote: ... the washed beads were re-suspended in 150 µl trypsin digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... using 3 µl of the in vitro translation reaction mixture in 150 µl of luciferase assay reagent (Promega, Fitchburg, WI). The graphs in Fig ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... luminescence was read by removing 150 µL of media and adding 50 µL of substrate from the Nano-Glo Luciferase Assay System (N1110, Promega). The mixture was incubated for 3 minutes in a white-walled plate before analysis with a SpectraMax M3 plate reader (Molecular Devices).
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bioRxiv - Molecular Biology 2024Quote: ... Powdered plant material (150 mg) was aliquoted and extracted with 1.25 mL hexadecyltrimethylammonium bromide (CTAB) buffer (MC1411, Promega, Madison, WI) containing proteinase K (ref 25530049 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a set of cells seeded in parallel were lysed and measured using 40 μL CellTiter-Glo luminescent cell viability reagent (Promega product code G7570) per well as per the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2020Quote: ... The reverse transcriptase M-MLV RT as well as the T4 DNA ligase were obtained from Promega. The pGEM-T Vector kit was purchased from Promega ...
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bioRxiv - Synthetic Biology 2019Quote: ... ligated for 3 h at room temperature (RT) or overnight at 4°C using T4 ligase (Promega), and propagated in E.coli XL 10 Gold cells (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... cell plates were equilibrated to RT for one hour and CellTiter-GloTM Luminescence Cell Viability Reagent (Promega) was added to the cell suspension ...
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bioRxiv - Neuroscience 2023Quote: ... The revers transcription (RT) reaction was performed by M-MLV reverse transcription kit (Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Diluted RNA was assayed for AGO2 transcript using the GoTaq 1-Step RT-qPCR System (Promega A6020) with primers CTGGCTCCAGGGGACAAG (Forward primer ...
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bioRxiv - Microbiology 2020Quote: ... PCR products (1.5Kb) of 16S rRNA gene were cleaned with Gel and PCR Clean-Up system (Promega, USA), and quantified by NenoDrop ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) kit ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) kit for Sanger sequencing.
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bioRxiv - Microbiology 2020Quote: ... PCR products were pooled and purified with Wizard Gel and PCR Clean-up Kit (Promega, Madison, Wisconsin, USA). Purified PCR products were sequenced at Michigan State University ...
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bioRxiv - Microbiology 2022Quote: ... After purification of the PCR product with Wizard® SV Gel and PCR Clean-Up System (Promega, UK), the arsM amplicon was sequenced at Microsynth (Balgach ...
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bioRxiv - Cancer Biology 2022Quote: ... Wizard® SV Gel and the PCR Clean-up System were used to clean the PCR products (Promega). Next ...
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bioRxiv - Genetics 2019Quote: ... The PCR product was purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) according to manufacturer instructions.
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was cleaned using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI), and samples were submitted to the Roy J ...
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bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were run using 2 µL of extracted DNA in GoTaq® PCR Master Mix (Promega) and the following cycling protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR fragment was subsequently purified using the Wizard SV Gel and PCR Clean-Up System (A9281 - Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was subsequently performed using 1 μg total RNA and oligo-dT primers (C110A, Promega, Madison, WI). Transcript levels were assessed via real-time quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2019Quote: ... 500ng of total RNAs were reverse-transcribed using SuperScript IV reverse transcriptase (ThermoFischer Scientific) and random primers (Promega). cDNA levels were measured using real-time quantitative PCR with the Power SYBR Green PCR master mix (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Primers were designed using the Flexi Vector primer design tool http://www.promega.com/resources/tools/flexi-vector-primer-design-tool/ and cloning was performed using Flexi cloning protocol (Promega). Briefly ...
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bioRxiv - Genetics 2022Quote: ... The DNM1 amplification was performed with specific primers pair (DNM1_Forward 5’-GGGTCTTGTACGGAGCAGGG-3’ and DNM1_Reverse 5’-GAGTCAGATAGTAAGGGCAAGCAC-3’) using Pfu DNA polymerase (Promega). After the first amplification to select DNM1 fragment of 761 bp ...
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bioRxiv - Bioengineering 2019Quote: ... a DNA fragment of 1191 bp was amplified using the pair of primers F_GFPseq / R_T0ter (Supplementary Table S6) with the GoTaq Flexi DNA polymerase (Promega) by colony PCR following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... and the first-strand cDNAs were synthesized using random primers and M-MLV reverse transcriptase (Promega, Madison, WI). qPCR was done by using Roche LightCycler 480 real-time PCR system (Roche ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs were synthesized from 2μg total RNA using oligo(dT) primers and M-MLV reverse transcriptase system (Promega). cDNA was used as template for quantitative PCR using a Bio-Rad CFX-96 real time PCR apparatus and SYBR green master mix (Dongsheng Biotech) ...
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bioRxiv - Cell Biology 2021Quote: ... was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega). KAPA Probe Fast qPCR Master Mix (2X ...
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bioRxiv - Plant Biology 2023Quote: ... Gene of interest was amplified using designed primers (Supplementary Table 8) and cloned into pGEMT-Easy vector (Promega). Using the DIG RNA Labelling Kit (Roche Diagnostics) ...
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bioRxiv - Plant Biology 2023Quote: ... Transcripts of interest were amplified using designed primers (Supplementary Table 4) and cloned into pGEMT-Easy vector (Promega). Digoxigenin-labelled antisense and sense RNA probes were transcribed from T7 or SP6 promoter of pGEMT-Easy vector (Promega ...
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bioRxiv - Microbiology 2023Quote: ... 2 ng of each purified epicPCR product was amplified using qacE_F (0.4 μM) and AP28_short (0.4 μM) primers in GoTaq polymerase master mix (Promega, United States) under the following thermocycling conditions ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rRNA region was amplified with the standard bacterial primers 335F and 769R with GoTaq Mastermix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of each primer (10 μM) and 12.5 μL of GoTaq Green Master mix (Promega®, USA). The PCR was run in a thermocycler (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... PCR for Pax7-nGFP was performed using primer Pax7nGFP.F/ Pax7nGFP.R and GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Cell Biology 2023Quote: ... Dystrophin gene editing was verified by PCR for mouse dystrophin using primers Dmd_i22-i23.F/ Dmd_i22-i23.Rand GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with primers Dmd_e22-e24.F / Dmd_e22-e24.R using GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Developmental Biology 2023Quote: ... The reverse transcription was performed using an oligo-dT primer and the ImRrom-II Reverse Transcription System (Promega). The ABI 7700 Sequence Detection System (PE Applied-Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Specific amplicons were prepared using several primer pairs (Table 1) in combination with GoTaq© DNA Polymerase (Promega).
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bioRxiv - Neuroscience 2019Quote: ... The PCR DNAs were gel purified (Promega Wizard SV Gel and PCR Clean-up System #A9282 ...