Labshake search
Citations for Promega :
851 - 900 of 2430 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... or PCR master mix (Promega, Wisconsin, USA). For ExTaq polymerase reactions ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR was performed using GoTaq Green (Promega) according to the vendor protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR was performed using GoTaq Green (Promega) according to the vendor protocol ...
-
bioRxiv - Microbiology 2022Quote: PCR clean-up system (Promega, catalog #: A9285).
-
bioRxiv - Microbiology 2019Quote: ... PCR products were digested with DpnI (Promega) to remove template plasmid ...
-
bioRxiv - Microbiology 2020Quote: ... Transformants were screened using GoTaq PCR (Promega) with universal M13F (−20 ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1 GoTaq™Flexi PCR buffer (Promega) and 20 pmol of each of the following four primers ...
-
bioRxiv - Microbiology 2022Quote: Colony PCR using GoTaq DNA polymerase (Promega) was performed on isolates not identified by MALDI-TOF MS ...
-
bioRxiv - Molecular Biology 2023Quote: ... a PCR using GoTaq G2 polymerase (Promega) was performed to specifically amplify the wild-type target locus ...
-
bioRxiv - Neuroscience 2023Quote: ... then PCR amplified with GoTaq MasterMix (Promega) and subsequently digested with a restriction enzyme to distinguish wild type and mutant alleles (Table 1) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed with GoTaq Flexi (Promega) for 30 cycles of ...
-
bioRxiv - Microbiology 2023Quote: PCR products and a standard ladder (Promega) were electrophoresed on 1% agarose gels in 1X TBE for 1 hr at 85V ...
-
bioRxiv - Microbiology 2024Quote: ... 6μL Promega PCR amplification-grade water (Promega) and 2 μL of the sample DNA (corresponding to about 20 ng) ...
-
bioRxiv - Genetics 2019Quote: For strand-specific RT-PCR of the D and U amplicons cDNA was generated from 150 ng of DNase-treated RNA using the GoScript™ Reverse Transcription System (Promega) with either the Downstream-F or Downstream-R primer ...
-
bioRxiv - Physiology 2019Quote: ... The intestines of five adult flies per replicate were collected in 150 μl of Glo Lysis Buffer (Promega, Mannheim, Germany, #E2661) and homogenized using a bead mill homogenizer (BioLab Products ...
-
bioRxiv - Biochemistry 2021Quote: ... HCT116 cells were plated on poly-L-lysine coated cover slips and transfected with ER calcium sensor ER-GCaMP6-150 using FuGENE HD (Promega Corp.). Experiments were carried out 1-2 days after transfection ...
-
bioRxiv - Cell Biology 2021Quote: ... or without (150 bp) one putative ETV2 binding site (Wei et al, 2010) were cloned into pGL3_Basic vector (Cat# E1751, Promega, Madison, WI). Primers Forward ...
-
bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
-
bioRxiv - Genomics 2023Quote: ... The reaction was stopped with the addition of 150 µL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and 2 µL Proteinase K (Promega, Cat # MC5005), then incubated at 65 °C overnight to reverse crosslinks ...
-
bioRxiv - Systems Biology 2023Quote: ... and 22 days post-surgery on a Lago X (Spectral Instruments Imaging) after isoflurane anesthesia and intraperitoneal administration of 150 µg D-luciferin (Promega, E1605) per gram body weight to monitor bioluminescence ...
-
bioRxiv - Neuroscience 2023Quote: The LDH release from day 40 FOs was measured by incubating individual FOs in 150 μl maturation medium ON and quantifying the LDH content in the medium using the LDH-Glo™ Cytotoxicity Assay (Promega) according to manufacturer instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... Beads were washed 5x with lysis buffer containing 150 mM NaCl and resuspended in 500 µl HDAC-Glo buffer (Promega, #G648A). Beads were serially diluted with HDAC-Glo buffer ...
-
bioRxiv - Genomics 2023Quote: ... The reaction was stopped with the addition of 150 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and 2 μL Proteinase K (Promega, Cat # MC5005), then incubated at 65 °C overnight to reverse crosslinks ...
-
bioRxiv - Microbiology 2019Quote: ... purified amplicons were inserted into plasmids and ligation reactions (10 μ1) were set up as per manufacturer’s instructions (Promega pGEM-T Easy vector system). After ligation ...
-
bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
-
bioRxiv - Cancer Biology 2020Quote: HPV16 integration into the IRF4 gene was confirmed by PCR using GoTaq Long PCR Master Mix kit (Promega U.S.), with 5’-AAGCATGTCAGACACGCAGA- 3’as a forward primer ...
-
Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... PCR products were gel purified with either Wizard® SV Gel and PCR Clean-Up System (Promega cat# A9281) or Isolate II PCR and Gel Kit (Bioline cat# BIO-52059 ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR reaction mixture was purified with the Wizard® SV Gel PCR Clean-Up System (Promega Corporation, WI, USA). One Shot® TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
-
bioRxiv - Biochemistry 2023Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
-
bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the WizardTM SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) following the manufactures instructions ...
-
bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control ...
-
bioRxiv - Microbiology 2023Quote: ... PCR reactions were gel-extracted using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison WI, USA) and sequenced by GENEWIZ (Leipzig ...
-
bioRxiv - Bioengineering 2023Quote: ... The obtained PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (A928, Promega) and blunt end ligation was performed followed by electroporation of freshly made competent cells [81] to yield 3.6×107 total transformants ...
-
bioRxiv - Synthetic Biology 2024Quote: DNA purification from PCR mixture was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
-
bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
-
bioRxiv - Plant Biology 2021Quote: ... One microgram of total RNA was reverse transcribed using an oligo(dT)15 and the MMLV-RT (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Physiology 2021Quote: ... The resultant cDNA was assessed by RT-qPCR using SYBR® Green Supermix 2x qPCR master mix (Promega) in a ABI PRISM 7900HT Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
-
bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
-
bioRxiv - Molecular Biology 2023Quote: A total of 10 µg RNA was taken from each sample for reverse transcription (RT) using GoScript (Promega) reverse transcriptase (5 µg for RT ...
-
bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the housekeeper gene Hprt (ID: Mm03024075_m1) using GoTaq Probe qPCR and RT-qPCR System (Promega, Madison, WI) and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis and qPCR were performed using the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) with an ABI Prism 7900HT Sequence Detection System (Applied Biosystems ...
-
bioRxiv - Physiology 2024Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...