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Citations for Promega :
901 - 950 of 3351 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed in 50 μl final volume using 2 U of GoTaq Polymerase (Promega) and 100 ng of Nichols DNA template ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into the XhoI and HindIII sites in the psiCHECK™-2 Vector (Promega) resulting in the plasmid pTal1-Luc ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μg of total RNA was used for cDNA synthesis with AMV reverse Transcriptase (Promega) together with 20 pmol of oligonucleotides O135 (5’-TAGCGGCTGATGTTGAACTG-3’ ...
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bioRxiv - Immunology 2022Quote: ... transfected with ISD (21) at 2 µ g per dish using ViaFect (Promega, Madison, WI) at 3 µ L per 1 µ g of ISD based on manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... transfection with ISD (21) at 2 µ g per dish using ViaFect (Promega, Madison, WI) at 3 µ L per 1 µ g of ISD based on manufacturer recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were subjected to digestion with LysC (Wako) for 2 h and trypsin (Promega) overnight at 37°C at a 1:50 enzyme ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of the resulting mRNA was mixed with 1 μl of FluoroTect GreenLys (Promega) and 3 μl of the wheat germ extract and then transferred under 50 μl of the translation buffer (1x SUB-AMIX SGC ...
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bioRxiv - Cancer Biology 2019Quote: ... Luciferase and Renilla activities were measured by Biotek Synergy 2 plate reader (Winooski, VT) using the Dual-luciferase assay kit (Promega, Madison, WI).
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bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned downstream of Renilla luciferase of psiCHECK(TM)-2 vector (Promega) using XhoI and NotI sites.
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bioRxiv - Microbiology 2020Quote: ... cells were transiently transfected with 2 μg DNA using FuGENE® HD (Promega, Madison, WI). Whole-cell patch-clamp recordings were performed at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Seedlings were sprayed with 2 mM Luciferin (VivoGlo™ Luciferin, In Vivo Grade, Promega, Netherlands) one day before they were released either into continuous light or dark ...
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... 2-DG uptake was measured using a Glucose Uptake-Glo Assay kit (Promega, Madison, WI).
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bioRxiv - Microbiology 2022Quote: ... Once cooled the beads were digested overnight with 2 µg of Trypsin (Promega Catalog # V5111) at 37 °C and constant rocking.
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transfected with 2 µg of plasmids with FuGene HD transfection reagent (Promega #E2312) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 2 μg of bacmid DNA using Fugene HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Each DNA family was amplified with 2×GoTaq® Hot Start Green Master Mix (Promega) using 0.8–2.2 ng of germline and somatic DNA and the primer pair according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... A volume of 2 µL of the samples was diluted in Glo lysis buffer (Promega) and incubated in a 1:1 ratio for 3 min in the dark with NanoGlo substrate ...
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bioRxiv - Systems Biology 2023Quote: ... 2 mg of total RNA was used for cDNA synthesis with MMLV reverse transcriptase (Promega) in final 50 mL volume ...
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from 2 ug of total RNA using GoScript Reverse Transcriptase (Promega, #A5003) with random hexamers according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The mice received intraperitoneal (IP) injection of 2 milligrams of luciferin (Promega Corporation, Madison, WI) and subjected to luminescence imaging using an IVIS® Spectrum In Vivo Imaging System (PerkinElmer Inc ...
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bioRxiv - Systems Biology 2023Quote: ... and 500 µL per sample were digested using 2 µg Trypsin/LysC (Promega, Cat#V5072). After 17 h of incubation at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... 2 mg of total RNA was used for cDNA synthesis with MMLV reverse transcriptase (Promega) in final 50 mL volume ...
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bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
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bioRxiv - Biochemistry 2023Quote: ... Samples were again diluted to 2 M urea and digested with 1 µg trypsin (Promega) (1/100 ...
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bioRxiv - Systems Biology 2023Quote: The transfections were performed with a 1 mg DNA: 2 ml Fugene HD (Promega E2311) ratio ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0) containing 2 nM 11S and 1/250 Nano-Glo Luciferase Assay Reagent (Promega) was added to the cells and background luminescence read for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... cells were transiently transfected with 2 µg DNA by lipofection (FuGENEⓇ HD, Promega, Madison, WI).
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated at 37°C for 1 hour and then proteolytic digestion was performed with LysC (Wako) for 4 hours and trypsin (Promega) overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using 1:100 (w/w) LysC (Wako Chemicals) for 4 h at 37°C and then finalised with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was then diluted to 4 mL with 50 mM ammonium bicarbonate and subjected to overnight digestion at 37°C with trypsin (Promega) using an enzyme to substrate ratio of 1:200 (w/w) ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated with the following primary antibodies diluted in TBS-T overnight at 4°C: mouse anti-HaloTag (1:5,000; Promega, G9211), mouse anti-Flag M2 (1:2000 ...
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bioRxiv - Cancer Biology 2022Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 µm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Immunology 2021Quote: ... 1 mM L-Glutamin and 250 μM Luziferin D (Promega, Madison, USA). Cells were incubated 12-16 hours in a humidified incubator at 37°C and 5% CO2 to adhere to the bottom of the plate ...
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bioRxiv - Plant Biology 2021Quote: ... Plants were pre-sprayed with 1mM D-luciferin (Promega, Fitchburg, Wl, USA) one day before imaging to deplete accumulated LUC (de Ruijter ...
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bioRxiv - Biochemistry 2021Quote: ... DNA modification enzymes and isopropyl β-D-thiogalactoside (IPTG) were from Promega. Oligonucleotides and molecular weight standards for native gel electrophoresis were supplied by Sigma ...