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Citations for Promega :
801 - 850 of 3351 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 3’UTR Wild and 3’UTR-Mutant were incorporated into XbaI restriction site of PGL3-control vector (Promega) expressing firefly luciferase ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μL of Cell Titer Glo (Promega) were added to each well ...
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bioRxiv - Zoology 2021Quote: ... and 3% 20mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact.
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bioRxiv - Cell Biology 2022Quote: ... We added 3 μg Trypsin Gold (Promega) to each sample for digestion and incubated samples at 37°C overnight (approximately 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... 3 μL of dNTPs (20mM, Promega®), 5 μL of buffer (5x ...
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bioRxiv - Microbiology 2022Quote: ... 3 μL of dNTPs (20mM, Promega®), 5 μL of buffer (5x ...
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bioRxiv - Zoology 2020Quote: ... and 3% 20mg/ml Proteinase K (Promega). Many additional photos of spicules and sections are available in the supplementary data that accompanies this paper ...
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bioRxiv - Genetics 2020Quote: ... 3 μl of RNasin ribonuclease inhibitor (Promega) per ml of extraction buffer] ...
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bioRxiv - Molecular Biology 2022Quote: ... For SUMO1-3 thrombin (16 U; Promega) was used for cleavage in buffer (20 mM Tris-HCl pH 8.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μl MgCl 25 mM (Promega A3511), 0.2 mM dNTPs ...
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bioRxiv - Zoology 2022Quote: ... and 3% 20mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
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bioRxiv - Zoology 2024Quote: ... and 3% 20mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
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bioRxiv - Cell Biology 2023Quote: ... 3 ul of RNasin Ribonuclease Inhibitor (Promega) was added to the beads and incubated for 2 hours at 4°C/3rpm ...
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bioRxiv - Biophysics 2022Quote: ... spheroplast/sucrose buffer suspension was added to 500 μL warm (42 °C) agarose solution (low melting point agarose, V2831 Promega, 2% w/v in sucrose buffer) using a cut pipette tip ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the secondary antibody for 1 h at +4 °C (anti-rabbit IgG-HRP, Promega 65-6120). The Pierce® enhanced chemiluminescence substrate (Thermo-Fischer Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of the digested pGEM-T easy vector and 5’ fragments was carried out overnight at 4 °C using 0.5 µl (1.5 U) of T4 DNA Ligase (Promega). pGEM-T easy vector containing the 5’ fragment (5’-pGEM-T ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions (3 μL) were incubated for 60 min at 37 °C and quantified with the Nano-glo assay system (Promega).
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bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns with a 3 kDa molecular weight cutoff and digested separately overnight using trypsin chymotrypsin or Glu-C (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Cancer Biology 2021Quote: The full length 3’ UTR of c-Myc was cloned into the pmirGLO Dual-Luciferase miRNA target expression vector (Promega). Luciferase activity was assayed in transfected cells using the Dual-Luciferase Reporter Assay (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were sequentially digested with LysC (1:50, w/w; FUJIFILM Wako) for 3 h at 37 °C and trypsin (50:1, w/w; Promega) overnight at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were reduced by dithiothreitol and alkylated by iodoacetamide in the dark at room temperature, followed by in-solution digestion sequentially using lysyl endopeptidase (Lys-C, Wako) for 3 h and trypsin (Promega) overnight at room temperature as previously described (Paul et al. ...
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 3days before CellTiter-Glo (CTG) assays were performed as per the manufacturer’s instructions (Promega). Luminescence was read using a Molecular Devices SpectraMax L plate reader.
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bioRxiv - Molecular Biology 2024Quote: ... The coding sequence of NanoLuc was amplified by PCR using the primers 5-NanoL and 3-NanoL from pNL1.1.CMV plasmid (Promega). The resulting fragment was digested with BamHI and NotI enzymes and cloned into pEGFP-N1 digested with the same enzymes ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Genetics 2019Quote: ... total RNA was treated with 2 U of RQ1 RNase-Free DNase (Promega, USA) at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: - A combination of KAPA HotStart ReadyMix and Pfu DNA Polymerase (2 U/μL, Promega) was used in some tests to try to increase enzyme processivity ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega). CSV files were exported onto a USB flash drive for analysis.
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bioRxiv - Systems Biology 2020Quote: ... The transfections were performed with 1 μg DNA: 2 μl Fugene HD (Promega E2311) ratio ...
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bioRxiv - Physiology 2021Quote: ... and simultaneously transfected with 2 μg of DNA using Fugene HD (catalog #E2312, Promega), accordingly to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).
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bioRxiv - Microbiology 2022Quote: ... 2 cm strips of intestinal tissues were embedded in low melting point agarose (Promega) to enrich for well-oriented crypt-villus units ...
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bioRxiv - Plant Biology 2021Quote: ... http://cshprotocols.cshlp.org/content/2009/2/pdb.prot5110.abstract) with trypsin (porcine, side chain protected; Promega). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... and the pre-cleared lysate was supplemented with 2 µl of RNasin Plus (Promega), added onto the freshly prepared ...
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bioRxiv - Biophysics 2020Quote: ... containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cAMP stock solution (Promega). The cells were then incubated for 2 hours at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... Two days after transfection with 2 to 2.5 μg DNA using Fugene HD (Promega), confocal images were acquired using an FV1000 confocal laser scanning microscope (Olympus ...
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bioRxiv - Microbiology 2021Quote: ... We synthesized cDNA from 2 μL of RNA using M-MLV reverse transcriptase (Promega) and random hexamers ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Luminescence was initiated by addition of a mixture of 2 μM coelenterazine-h (Promega) and 40 μM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Physiology 2021Quote: ... allowing measurement of 2-deoxyglucose-6-phosphate using the Glucose Uptake-Glo assay (Promega) and a FLUOstar Omega luminescence plate reader.
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bioRxiv - Microbiology 2020Quote: ... RNA (2 μg) was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega). Conventional PCR was used with the primer (Table 1 ...
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bioRxiv - Immunology 2021Quote: ... F(ab′)2 fragments were generated after cleavage of IgG with IdeS protease (Promega) and then purified using TALON metal affinity resin (Takara ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with 2 μg of the respective guideRNA plasmids using FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... with 2 uM Ponasterone A overnight and incubated with JF646 HaloTag ligand (Promega, GA1120) 15 minutes before imaging ...
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bioRxiv - Immunology 2022Quote: ... 1-2 µg RNA was reverse transcribed using AMV Reverse Transcriptase (Promega, Madison, WI). Primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Plant Biology 2023Quote: ... Two (2) μg of total RNA were used for cDNA synthesis with MMLV (Promega) and random hexamers (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... or a combination of 2 μg of trypsin and 0.2 μg of LysC (Promega). Samples were incubated at 37 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Microbiology 2023Quote: ... and TCID50 equivalents determined using a SARS-CoV-2 E gene detection kit (Promega) based upon the Berlin primer/ probe set (70).