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Citations for Promega :
851 - 900 of 1454 citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Apoptosis of cells cultured in vitro were assessed using a cleaved caspase3/7 activity kit following the manufacturer’s instructions (Promega, cat#8090). Briefly ...
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bioRxiv - Genomics 2023Quote: The IRF3/IRF7 luciferase reporter was constructed by subcloning 3x IRF3/7 binding element (GTCAGGAGAAGGAAACCTTC) into the Sal I and HindIII sites in the pGL3-basic (Promega E1751) backbone vector ...
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bioRxiv - Plant Biology 2023Quote: ... The final pellet was dried at room temperature and resuspended in 500 µL of [7 M urea (Promega, Madison, WI, USA), 2 M thiourea (Sigma-Aldrich Corp. ...
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bioRxiv - Cancer Biology 2023Quote: ... cytotoxicity and apoptosis were measured 48h and 7 days after the knock-down using ApoTox-Glo triplex assay kit (Promega,G6320) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: The FASP method[7] was used to digest urine protein with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA). One hundred micrograms of urine protein was added in the membrane of a 10KD ultrafiltration tube (Pall ...
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bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
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bioRxiv - Microbiology 2022Quote: ... DNA from the saliva samples (n=67, one missing) was extracted using the ReliaPrep Blood gDNA Miniprep (Promega, USA). For microbiome profiling ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by applying 20 µl of CellTiter 96® AQueous One Solution Reagent (Promega, Madison WI) to each well and incubating for 2 h at 25°C ...
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bioRxiv - Bioengineering 2020Quote: ... Cell viability was determined using the MTS assay (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, G3580; Promega) according to the manufacturer’s instructions and was calculated as a percentage of the negative control ...
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bioRxiv - Biochemistry 2020Quote: ... Luciferase activity was measured using ONE-Glo™ Luciferase Assay System according to the manufacture’s protocol (Promega, Madison, WI). The IC50 values were determined by log(inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
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bioRxiv - Microbiology 2021Quote: ... Infections of 50 000 cells were performed at MOI 2 for HCoV-229E-Renilla (as measured on Huh7.5.1 cells) and infection efficiency was analyzed one day later by measuring Renilla activity (Renilla Luciferase Assay System, Promega).
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bioRxiv - Microbiology 2021Quote: ... The pellets were then subjected to one freeze-thaw cycle before being resuspended in Passive Lysis Buffer (Promega; E1910). One scoop of 0.15 mm zirconium oxide beads (Next Advance ...
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bioRxiv - Bioengineering 2020Quote: ... and an absorbance LDH assay (Lactate dehydrogenase assay, CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit from Promega, G7890) was used to determine the level of cytotoxicity caused by membrane compromisation ...
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bioRxiv - Pathology 2022Quote: ... G490 5 × All-In-One RT MasterMix (abm) was used for reverse transcription according to the manufacturer’s protocol (Promega). The qRT-PCR analysis was performed in 96-well plates using the BIO-RAD CFX96 detection system ...
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bioRxiv - Immunology 2022Quote: ... Transduced cells were incubated for 24 hours at 37°C/5% CO2 and luminescence measured by addition of 40μl of ONE-Glo reagent (Promega) with detection using a Tecan Spark plate reader ...
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bioRxiv - Cancer Biology 2022Quote: The cell viability assay was done using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Milano, Italy). It is a colorimetric method for accessing the number of viable cell in proliferation ...
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bioRxiv - Cancer Biology 2022Quote: The cell proliferation of U87MG and H4 was observed using CellTiter 96® AQueous One Solution Proliferation Assay (Promega). Cells were cultured in a 96-well plate for 72 hours under activation and AZD8055 treatment conditions ...
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bioRxiv - Pathology 2020Quote: ... One thousand ng of RNA was reverse transcribed to cDNA using reverse transcription reagents (Go Script, Promega, Madison, WI) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... CellTiter 96® AQueous One Solution Cell Proliferation Assay and Dual-Glo® Luciferase Assay were purchased from Promega Corporation.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cell viability was monitored using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA). VeroE6 cells or Huh7.5 cells were plated at 10,000 or 8,000 cells per well of flat bottom 96-well plates ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was measured by MTS/PES One Solution Cell Titer Assay following manufacturer’s protocol (Promega Corp, Madison, WI).
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bioRxiv - Genomics 2021Quote: ... DNA quantity and quality was verified with the Quantus fluorometer assay (QuantiFluor ONE dsDNA Dye, Promega, Madison, WI, USA) and Femto Pulse® Automated Pulsed Field (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... a colorimetric MTS assay was performed on HUVECs following the manufacturer’s protocol (CellTiter 96 AQ ueous One Solution Cell Proliferation Assay, Promega) in 96 wells ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability was quantified using an MTS assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay, Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined using Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Data was generated as means of six replicates from two independent experiments +/- SEM.
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bioRxiv - Molecular Biology 2022Quote: The activity of the NF-κB luciferase reporter cell line was measured according to the manufacturer’s protocol (ONE-GloTM Promega) into 96-well plates with white walls and bottom to reduce the cross-contamination of the signal between wells ...
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bioRxiv - Microbiology 2022Quote: ... Dual Luciferase reporter assay kit and CellTiter96 Aqueous one solution cell proliferation assay kits were from Promega (Madison, USA). Non-targeting siRNA (Catalog no ...
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bioRxiv - Microbiology 2023Quote: ... The samples were eluted with TN buffer (10 mM Tris-HCl, pH 8.0, 50 mM NaCl) and quantified using the QuantiFluor ONE dsDNA System (Promega). The resulting barcoded DNA samples were combined in approximately equimolar amounts (a total of 100 femtomoles in 10 µl of TN buffer) ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined using Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Data was generated as means of six replicates from two independent experiments +/- SEM.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Cat. #G3580, Promega). All experiments were performed in biological triplicates.
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bioRxiv - Bioengineering 2023Quote: ... One microgram of RNA was converted to cDNA using ImProm-II™ Reverse Transcription System (PROMEGA, Madison, WI, USA) following the manufacturer’s instructions performed on T100™ Thermal Cycler (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... The number of viable cells was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega G3580) and absorbance at 490 nm was measured by a plate reader (CLARIOstar ...
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bioRxiv - Molecular Biology 2023Quote: ... The MTS assay was carried out using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was digested overnight at 37 °C with one or more of the following enzymes separately: trypsin (Promega), Lys-C (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Cat. #G3580, Promega) at 24 ...
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bioRxiv - Neuroscience 2023Quote: ... Firefly and Renilla luminescence activities were measured one day after transfection using the Dual Luciferase Reporter Assay System (Promega), following the passive cell lysis protocol.
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bioRxiv - Neuroscience 2024Quote: ... The pool was quantified by Fluorometry using using the QuantiFluor ONE dsDNA System (Cat# E4871, Promega, Madison, WI, USA) on Quantus instrument (Promega) ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IL-12 and TNFα were analyzed by semiquantitative PCR (SYBR Green assay Go Taq qPCR Master Mix; Promega) with specific primers (Metabion ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured after 12 days using CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... eluted in 12 µl of nuclease free water and quantify using the dsDNA QuantiFluor Dye System (Promega, #E2670).
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bioRxiv - Cell Biology 2023Quote: ... Samples were then acidified with 12-21% aqueous phosphoric acid prior to digestion with Trypsin/Lys-C (Promega) and then added immediately to an S-Trap column (Protifi ...
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bioRxiv - Molecular Biology 2020Quote: ... of selected 3’UTRs into the pmirGLO vector (Promega, Southampton, UK) was used to generate 3’UTR luciferase reporters essentially as previously described (9) ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was transfected with 3 uL FuGENE 6 reagent (Promega) and 130 ng pcDNA3-mouseSTING in line with manufacturer’s protocols.
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bioRxiv - Microbiology 2021Quote: ... 3 μg of total RNA was treated with RQ1 DNase (Promega), and then purified by phenol chloroform extraction and ethanol precipitation ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly add 3 µL 1:1 water diluted digitonin (Promega G9441)) was added ...
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bioRxiv - Molecular Biology 2019Quote: A 3:1 ratio of FuGENE® HD Transfection Reagent (Promega) (μL ...
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bioRxiv - Immunology 2021Quote: ... then each well was diluted 1:3 with TE buffer (Promega). A 2.7 μL aliquot from each sample was mixed with 2.5 μL of SsoFast EvaGreen Supermix with Low Rox (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... or 250 ng DNA (Nup54-mEGFP) and 3 µL FuGENE6 (Promega) in Opti-Mem I (Gibco ...