Labshake search
Citations for Promega :
651 - 700 of 1454 citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, G3582).
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bioRxiv - Synthetic Biology 2024Quote: Signal of the Fluc reporter was quantified with the ONE-Glo Luciferase Assay System (Promega) and Nluc reporter was quantified with Nano-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... at one week and at two weeks and measured by CellTiter-Glo (Promega #PR-G7573) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by measuring luciferase activities using ONE-Glo™ EX Luciferase Assay System (Promega E8110) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cell proliferation was measured using CellTiter 96 AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and one day later the cells were transfected with pISRE-luc and pTK-RL (Promega) using TransIT®-LT1 Transfection Reagent ...
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bioRxiv - Molecular Biology 2024Quote: MTS assays were undertaken using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega) or Abcam MTS assay kit (ab197010) ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability and proliferation was assayed using CellTiter 96 Aqueous One Solution Reagent (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Prior to overnight trypsin-digest (12 ng/ul trypsin in 50 mM ABC, Promega) at 37° C ...
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bioRxiv - Cancer Biology 2020Quote: ... and dissociated in 50% Ham’s-12/DMEM medium containing 0.075 mg/mL liberase (Promega) for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability was measured 7 days after lentiviral infection using the CellTiter-Glo reagent (Promega, Madison, WI).
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bioRxiv - Developmental Biology 2022Quote: ... melanogaster genomic DNA (7 – 8.5 kb fragments) and TA cloning into a PGEMT-Easy vector (Promega #A1360) (primers listed in Table S5) ...
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bioRxiv - Cancer Biology 2023Quote: ... day 5 and day 7 for each condition using the CellTiter-Glo (CTG) luminescence-based assay (Promega). Diluted CTG reagent (100 uL 1:4 CTG reagent to PBS ...
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bioRxiv - Microbiology 2021Quote: ... Standard RNA was synthesized from a partial region of the GPC gene using the forward (5’-TAATACGACTCACTATAGGGCCAACCTTTTTGCAGGAGGC-3’) and reverse (5’-AGCTTCTTCTGTGCAGGATCTTCCTGCAAGCGCTAGGAAT-3’) primers and the T7 RNA polymerase (Promega), as previously described (Pemba et al. ...
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bioRxiv - Microbiology 2022Quote: ... The region of recombination was amplified using primers PV3-F2 (5′-CTCCAAAGTCCGCATTTACA-3′) and PV1-R2 (5′-ATCAGGTTGGTTGCTACA-3′) and Taq polymerase (Promega) with an initial denaturing at 95°C for 2 min ...
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bioRxiv - Cell Biology 2019Quote: ... The full-length coding sequence of murine Tmem98 was amplified from a mix of murine embryonic and murine keratinocyte cDNA using forward 5’-AAAAAGCTTGCCATGGAGACTGTGGTGATCGTC-3’ and reverse 5’-TTTTGAATTCTTAAATGGCCGACTGTTCCTGCAGGAAGC-3’ primers and cloned into pSP72 (Promega) as a HindIII/EcoRI fragment ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting 3’UTR-Wild and 3’UTR-Mutant genes were inserted into the XbaI restriction site of pGL3-control vector (Promega) expressing firefly luciferase ...
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bioRxiv - Microbiology 2020Quote: ... 5 pmol of each primer (C1105 5’-GGTTCATCGACATCTCCGCG-3’ and C1106 5’-AGGTCGCTGCGCATGCCAATC-3’) and 1.25 units of GoTaq® DNA polymerase (Promega). The cycling conditions were as follows ...
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bioRxiv - Cell Biology 2021Quote: ... a ~600 bp mouse ATF6β cDNA fragment was PCR-amplified using 5’-AACAGGAAGGTTGTCTGCATCAT-3’ and 5’-GTATCCTCCCTCCGGTCAAT-3’ primers and inserted into the pGEM-T vector (Promega). The plasmid was linearized using EcoRV and ApaI to synthesize the antisense and sense probe ...
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bioRxiv - Cancer Biology 2022Quote: SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay.
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bioRxiv - Cancer Biology 2019Quote: ... Sense (5’-AGGAAACTGAAAAACAGAGTAGCAGC-3’) and antisense (5’-TCCTTCTGGGTAGACCTCTGG-3’) primers were used in a standard GoTaq Green PCR reaction (Promega) to amplify a region spanning the 26-nucleotide intron that includes a single PstI restriction site ...
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bioRxiv - Genetics 2021Quote: ... A ~1 kb fragment of the blm cDNA was cloned using primers blm-F – 5’-GGAGTCGAAACACCTGGTGGTA-3’ and blm-R – 5’-CTCATCAATGACCAAGCGAGCC-3’ into pGEM-T vector (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The V4 region of the 16S rRNA gene was amplified using the universal primers 515F (5′-GTG CCA GCM GCC GCG GTA A-3′) and 806R (5′-GGA CTA CNN GGG TAT CTA AT-3′) [26] with Taq&Load MasterMix (Promega). PCR reactions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded on 24 mm glass coverslips and transfected with 2 µg plasmid DNA (1:1.3:3 ratio LAMP1:ER:opto-kinesin) and Fugene6 transfection reagent (Promega 1:3) for 20-30 h prior to imaging ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 18 RNA extractions (3 morphs × 3 tissues × 2 biological replicates) were performed using the SV Total RNA Isolation System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... and digested with 3 μg LysC (Wako) O/N at 37°C and then with 3 μg of trypsin (Promega) for eight hours at 37°C following FASP procedure (Filter-aided sample preparation 48) ...
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bioRxiv - Biochemistry 2022Quote: ... The 400 base pair region surrounding the sgRNA target was then amplified by PCR (forward primer: 5’-CCCAGAGAGGAGGCTGTAGA-3’; reverse primer: 5’-AAAGGCCTCCCAGGGGTTAT-3’) with GoTaq DNA Polymerase (Promega). The resulting PCR product was cloned into the pCR 4-TOPO vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: RT reaction mix was set up and cDNA products were then amplified by PCR (25 cycles) with specific antigenome forward (5’-CATTCTACGAGCCGGTGCGC-3’) and reverse (5’-TAGACGTAGACCCCCAGAGTC-3’) primers using the GoTaq DNA polymerase (Promega) and analysed on a 1.5% agarose gel for analysis.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 3’-UTR derived from pNL1.1[Nluc] vector (Promega), and 50 nt poly(A ...
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Systems Biology 2020Quote: ... and T4 DNA ligase (3 U/μί, Promega) were applied to assemble all of the synthetic promoter blocks sequentially and simultaneously into the firefly reporter vector backbone in a one-pot reaction ...
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bioRxiv - Neuroscience 2019Quote: ... organoids were embedded in 3% agarose (Promega, #V3125), sectioned at 120-µm thickness and collected in 30% sucrose in 1× PBS for cryopreservation ...
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bioRxiv - Biochemistry 2021Quote: ... 1.8 nM 3 kb supercoiled pGEMT plasmid (Promega), and 20 nM MMTV intasomes in a final volume of 15 µL ...
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bioRxiv - Zoology 2023Quote: ... and 3% 20 mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... The mixture was then and incubated for 7min at 55°C followed by an additional 7 minute incubation with 3.5µl of SDS 0.2% (Promega, #V6551) to ensure that Tn5 was dissociated from the cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... ATP levels at day 0 and day 7 were measured using CellTiter-Glo Luminescent Cell Viability Assay (Promega). For analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... One μg of total RNA was DNase treated following the RQ1 RNase-Free DNase protocol (Promega) and then reverse transcribed with a mixture of random primers and oligo(dT ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell proliferation was assessed using the CellTiter96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Promega).
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bioRxiv - Immunology 2019Quote: ... the plates were assayed using the CytoTox-ONE Homogenous Membrane Integrity Assay (#G7890, Promega, Madison, WI) as per the kit protocol ...
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bioRxiv - Microbiology 2021Quote: ... Necrosis measurement was performed using CytoTox-One Homogeneous Membrane Integrity Assay according to manufacturer’s instructions (Promega). For Caco-2 cells (passage 30-45) ...
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bioRxiv - Biochemistry 2020Quote: ... Two control reactions were performed: one containing 8 units of HeLa nuclear extract provided by Promega as a positive control and the other containing no nuclear protein as a negative control ...
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bioRxiv - Bioengineering 2020Quote: ... Cell proliferation was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega Corporation) as per manufacturer’s instructions and quantified by absorbance at 490nm ...
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bioRxiv - Molecular Biology 2021Quote: ... One hundred nanograms of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA (Promega) was used for the library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS; Promega, Madison, WI, USA) was added and incubated 1−4 hours at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with CellTiter 96® AQueous One Cell Proliferation Assay solution (Promega, Wisconsin, U.S.A.) for 2 h at 37°C ...
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bioRxiv - Physiology 2022Quote: One tube was used for NAD/NADH quantification using the NAD/NADH Glo™ Assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... MTS assay was performed by adding 20 μl of reagent (Promega CellTiter 96 Aqueous One Solution) to each well and incubated at 37°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity was measured using a ONE-Glo™ EX Luciferase Assay System (Promega, Cat# E8130).