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Citations for Promega :
801 - 850 of 4810 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
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bioRxiv - Genomics 2020Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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bioRxiv - Cell Biology 2020Quote: ... The reduced and alkylated protein lysates were diluted using 50 mM Tris-Cl to obtain a final concentration of 2 M urea in solution followed by overnight digestion with trypsin (1:1000)(Promega). Peptides were then desalted using c-18 Sep-Pack columns (Waters) ...
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bioRxiv - Genetics 2020Quote: ... 1-2 μL of cDNA was used for 25 μL PCR reactions using the GoTaq Hot Start Master Mix (Promega). Cycling parameters consisted of an initial denaturation of 95°C for 2 min. ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were diluted 8x in 50 mM ammonium bicarbonate to reduce the urea concentration to 1 M and protein digestion was performed overnight at 37 C by addition of 2 µg of trypsin (Promega) per sample.
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bioRxiv - Neuroscience 2023Quote: ... samples were diluted with 50 mM NH4HCO3 to a final concentration of less than 2 M urea and were further digested overnight with 1:25 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... to a final urea concentration ˂ 2 M and then digested with modified trypsin (1:50 w/w) (Sequencing Grade, Promega) at 37°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours followed by another overnight digestion with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Microbiology 2022Quote: ... 40 µL of ONE-Glo reagent (Promega #E6110) added and luminescence measured using a Tecan Spark plate reader.
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bioRxiv - Microbiology 2022Quote: ... 40 µL of ONE-Glo reagent (Promega #E6110) added and luminescence measured using a Tecan Spark plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μl ONE-Glo™ luciferase reagent (Promega) was added to each well for 3-min incubation at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... 100 μl ONE-Glo™ luciferase reagent (Promega) was added to each well for 3-min incubation at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... using ONE-Glo™ Luciferase Assay System (Promega), according to the manufacturer’s specifications.
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bioRxiv - Cell Biology 2021Quote: ... ONE-Glo™ Luciferase Assay System (Promega, E6110), Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... One μg of Sequencing Modified Trypsin (Promega, V5117) was added and the samples were incubated at 37 °C for 16 hours under constant shaking (500 rpm) ...
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bioRxiv - Immunology 2022Quote: ... equal volume of One-Glo reagent (Promega; E6120) was added to culture medium for readout using BMG PHERastar-FS plate reader ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL One-Glo luciferase substrate solution (Promega) was added to each well prior to reading the signal on the Envision plate reader (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... before reconstituted luciferase reagent (ONE-Glo EX, Promega) was added to cells in culture medium ...
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bioRxiv - Microbiology 2021Quote: ... 60 μL of One-Glo-EX substrate (Promega) were added to each well and incubated on a plate shaker in the dark ...
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bioRxiv - Immunology 2021Quote: ... 40 µL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μL of ONE-Glo luciferase substrate (Promega) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... 40 µL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... CellTiter 96 AQueous One Solution (MTS reagent; Promega) was added and the plates incubated for a further 3 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... or One-Glo® assay (Promega, Cat #E6110). HEK-Blue IFN-α/β cells (InvivoGen ...
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bioRxiv - Molecular Biology 2022Quote: ... One microliter of DNase I (Promega, Madison, WI), 2 microliters of RNasin Plus were added to each sample and samples were incubated at 37°C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... One hundred ng SRE-Luc reporter (E134A, Promega), 300 ng Gαi or Gαi QL in pcDNA3.1+ ...
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bioRxiv - Genomics 2023Quote: The RNase ONE Ribonuclease (catalog num. M4261, Promega) was used at this point to eliminate the Biotin groups at the 3’ end by degrading only the single-strand RNA present into the mixture ...
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bioRxiv - Biochemistry 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added and cells were incubated at 37 °C for 10 minutes with shaking ...
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bioRxiv - Cancer Biology 2023Quote: ... with QuantiFluor® ONE dsDNA dye (Promega, E4870). Rotating incubation was done using a Tube Revolver Rotator from Thermo Scientific™ (88881002).
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bioRxiv - Cancer Biology 2023Quote: ... with QuantiFluor® ONE dsDNA dye (Promega, E4870). Temozolomide was purchased from Sigma-Aldrich (T2577).
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bioRxiv - Biochemistry 2023Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... One microgram sequence-grade modified trypsin (#V5111, Promega) was then added for on-bead trypsin digestion at 37°C for 16 h ...
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bioRxiv - Biochemistry 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
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bioRxiv - Microbiology 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to the cells and incubated for at least 5 minutes on a plate shaker in the dark ...
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bioRxiv - Microbiology 2020Quote: ... 5 % CO2 for 72 h before being washed with PBS and 200 µL Passive Lysis buffer (Promega, UK) added to each well ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Biochemistry 2020Quote: ... After the addition of 5 mM ATP the reaction was taken to a plate reader (Promega GloMax Navigator) and the luminescence collected for approximately 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... entire gel lanes were excised into 5 equal regions from top to bottom and digested with trypsin (Promega) as described (30) ...
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bioRxiv - Microbiology 2022Quote: ... Ten nanograms of template DNA was added to 5 µl Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units GoTaq DNA Polymerase (Promega ...
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bioRxiv - Cancer Biology 2019Quote: ... Dilute proteins were digested overnight with 5 μl of 25 ng/μl trypsin gold (Promega, Madison, WI, USA) and desalted with a ZipTipC18 pipette tip (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... zyxin(-/-) MEFs were transfected with 5 μg total DNA using the Fugene® 6 transfection reagent (E2691, Promega) and plated on glass coverslips.
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bioRxiv - Genomics 2019Quote: ... We then set up quantitative PCR reactions with 5 µL GoTaq qPCR Master Mix (Promega, Madison, WI, USA), 1 µL each forward and reverse primer at 5 µM ...
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bioRxiv - Immunology 2020Quote: ... Cell death after 5 h treatment was quantified by PI incorporation or using the Caspase Glo system (Promega) according to manufacturer instructions.