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Citations for Promega :
7601 - 7650 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... strain ADP were extracted using Wizard™ Genomic DNA Purification Kits following the standard protocol provided by Promega.
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bioRxiv - Genomics 2024Quote: ... (viii) checking libraries on the Bioanalyser for expected insert size and concentration (QuantiFluor® dsDNA System kit, Promega), (ix ...
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bioRxiv - Synthetic Biology 2022Quote: ... The chromosomal DNA of all strains was extracted using the Wizard® Genomic DNA Purification Kit (Promega, USA) and the integration of antibiotic resistance cassettes or reporter genetic constructs in the genome was confirmed by PCR under standard conditions using the GoTaq Green Master Mix (Promega ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from liquid nitrogen quick-frozen tissue using a Total RNA Extraction Kit (LS1040; Promega) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... genomic DNA was extracted from 2-wk old seedlings by using the Wizard Genomic DNA purification kit (Promega) and was subjected to PCR analysis by using a primer set consisting of a specific CaM35S promoter sequence and a gene-specific primer (Table S1) ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μg of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 μl containg 5 μL SYBR-green master mix (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs were prepared using the Ambion mMessage Machine kit using Sp6 (#AM1340) supplemented with RNAse Inhibitor (Promega #N251B) after plasmid linearization with Not1 ...
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bioRxiv - Cancer Biology 2023Quote: Isolation of genomic DNA from cultured cells was performed by Wizard genomic DNA isolation kit (Promega, WI, USA). Amplification of the barcodes was carried out by nested PCR ...
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bioRxiv - Evolutionary Biology 2023Quote: The total RNA of Lemna minor was extracted using Eastep® Super Total RNA Extraction Kit (Promega, USA) and reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was then isolated from the isopropanol/chloroform solution using the ReliaPrep RNA Cell Miniprep kit (Promega Z6010). For linear RNA depletion ...
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bioRxiv - Plant Biology 2023Quote: ... following the DNA extraction protocol published by (Mahuku 2004) and further purified with a DNA purification kit (PROMEGA). The quantity and quality of DNA were confirmed by electrophoresis on agarose gels stained with GelRed and visualized under UV light using a NanoDrop 1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA extraction was performed on the frozen cores using the Maxwell 16 LEV simplyRNA Tissue Kit (Promega, AS1280). Specimens were prepared for RNA-seq as described above and RNA quality was verified using Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: KO pool cells were collected and gDNA was extracted using Wizard® Genomic DNA Purification Kit (#A1120, Promega) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: The substrate of the nano-luciferase diluted at 1:100 (v/v) (furimazine, Kit from Promega (ref 1110)) was spread on seedling that grew on Agar plates ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the Maxwell® RSC simplyRNA Cells and RNase-free DNase Kit (Promega; AS1390) using an automated RNA/DNA isolation instrument (Maxwell ...
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bioRxiv - Neuroscience 2024Quote: ... 1.5 µg of total RNA was converted into single-stranded cDNA using the ImPromII reverse transcriptase kit (Promega) with random primers (0.5 µg/ml) ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from the whole body of dissected beetles using the Wizard Genomic DNA Purification Kit (Promega). The concentration of extracted DNA was estimated using a Qubit fluorimeter (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was isolated with Qiagen miniprep kits and reverse transcribed using ImProm-II reverse transcriptase (Promega, A3802) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Molecular Biology 2024Quote: The frozen tissues (∼25 mg) were homogenized in 400 µL of 1x reporter lysis buffer (#E1501 kit, Promega), and the lysates underwent 3 freeze thaw cycles (-80°C to 37°C) ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from the petals using the Maxwell RSC RNA Plant Kit (Promega, Madison, WI, USA), according to the manufacturer’s protocol with modifications ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from the leaves using a Maxwell 16 LEV Plant RNA Kit (Promega, WI, USA). The amount of RNA was quantified using the Quant-iT RNA Assay Kit broad range (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... adhering to the manufacturer’s instructions provided with the Wizard® Genomic DNA Purification Kit (Promega Corporation, United States). This extraction process included treatments with RNase and Proteinase K to remove potential RNA and protein contaminants ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were submitted for STR profiling (ECACC, case number 21579, using a Promega Powerplex 16 HS kit) and were routinely monitored for mycoplasma infection (MycoAlert® ...
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bioRxiv - Biochemistry 2024Quote: ... The virus titer was determined by the viability of infected SF9 cells using the MTS kit (Promega #G3580).
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bioRxiv - Genomics 2024Quote: ... High molecular weight DNA was extracted utilizing Wizard® HMW DNA Extraction Kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR mixtures were prepared as recommended by the GoTaq Flexi DNA polymerase kit protocol (Promega, Madison, Wisconsin, USA). The following temperature programme was used ...
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bioRxiv - Microbiology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in supernatants using CytoTox® Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and mouse cyclin B1 were generated using the Riboprobe in vitro Transcription Systems kit according to the manufacturer’s protocol (Promega). Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare) ...
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bioRxiv - Systems Biology 2022Quote: ... Sample concentrations were measured by incubating 1μl of cDNA from each well with 49μL of a fluorescent dsDNA dye (Quantifluor dsDNA kit, Promega E2670) and measured on a plate reader with fluorescent detectors (504nM Excitation/531nM Emission ...
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bioRxiv - Biochemistry 2022Quote: ... The splicing substrate XBP1u mRNA was prepared using a RiboMAX™ Large Scale RNA Production Systems—T7 kit (Promega) at 37°C for 3 hours ...
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bioRxiv - Genetics 2022Quote: ... the SEV hardware was replaced with the Low Elution Volume (LEV) hardware and the Simply-RNA Cell kit (Promega) was used ...
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bioRxiv - Neuroscience 2021Quote: Neuronal transfections were performed on DIV 7 using a calcium phosphate kit (ProFection Mammalian Transfection System, Cat # E1200, Promega), based on a previously described method (Sando et al. ...
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bioRxiv - Neuroscience 2020Quote: ... digoxigenin (DIG)-labeled riboprobes were generated with a commercial transcription kit (Riboprobe System, P1440, Promega, San Luis Obispo, CA) and DIG RNA labeling mix (Roche REF# 11277073910 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA of the renal cortex in cynomolgus monkeys was extracted using Maxwell RSC simplyRNA tissue kit (Promega, AS1340) by an automated Maxwell RSC Instrument (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: pCDH-WSB1 and pGL4.14-c-Myc promoter-luciferase plasmids as well as pCDH-WSB1 and pGL4.14-TCF/LEF1-luciferase plasmids were transfected on H460 and tested with the Luciferase reporter kit (Promega) in the same way ...
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bioRxiv - Genomics 2020Quote: ... Total RNA (1.5 μg) was reverse transcribed into cDNA using an ImPromII™ Reverse Transcriptase System kit (Promega, USA.) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2020Quote: ... venezuelae macrocolonies that were grown for 30 h at 30°C on MYM agar were pooled and resuspended in 200 µl ice-cold stop solution (5% phenol (pH 4.3) in 98% ethanol) and RNA was isolated using the SV Total RNA Isolation Kit (Promega). After elution ...
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bioRxiv - Cancer Biology 2020Quote: HPV16 integration into the IRF4 gene was confirmed by PCR using GoTaq Long PCR Master Mix kit (Promega U.S.), with 5’-AAGCATGTCAGACACGCAGA- 3’as a forward primer ...
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bioRxiv - Genomics 2020Quote: ... The nitrite concentrations were detected in culture supernatants using Griess reaction and a commercial kit from Promega (WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from high titer lysates using the Wizard® Clean-Up Kit (cat. # A7280, Promega, WI, USA). Sequencing libraries were constructed with the NEBNext® UltraTM II DNA Library Prep kit (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... The screened colony was later cultivated in YPD media and genomic DNA was extracted with Wizard genomic kits (Promega). Then the genomic samples were used as template for PCR verification of the integrated gene with gene-specific primers.
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bioRxiv - Cancer Biology 2020Quote: ... and DNA was digested using RQ1 RNase-Free DNase kit according to manufacturer’s instructions (Promega, Madison, WI, Cat#: M6101). Finally ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: Genomic DNA of Chlamydomonas cells was extracted and purified using a Wizard® Genomic DNA Purification Kit (Promega, Beijing) following the kit’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were lysed and assayed using the SteadyGlo reagent kit and a GloMax luminometer (Promega, Maddison, WI, USA). To measure HCVcc replication ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA was extracted using Maxwell® RSC Plant RNA or Maxwell® 16 miRNA Tissue Kit from Promega according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... after which cell viability was measured using the colorimetric CellTiter 96® Aqueous Non-Radioactive Cell Proliferation kit (Promega). The absorption at 495 nm was measured using a monochromatic filter in a multimode plate reader (Envision ...
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bioRxiv - Microbiology 2020Quote: ... These analyses were confirmed extracting genomic DNA (gDNA) using a Wizard® Genomic DNA Purification Kit (Promega, WI, USA) and total RNA was extracted with TRIzol (ThermoFisher Scientific Inc ...
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bioRxiv - Microbiology 2020Quote: ... The radioactive DNA probes used were prepared using Prime-a-Gene Labeling System kit (U1100, Promega, Madison, WI, USA), and radiolabeled dCTP (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: RNA extraction from FFPE specimens of chRCC was performed using the Maxwell® 16 Tissue DNA Purification Kit (Promega). RNA quality was assessed with the RNA Qubit™ RNA HS Assay Kit (ThermoFisher) ...