Labshake search
Citations for Promega :
701 - 750 of 904 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... RNA was extracted using either the Maxwell 16 LEV simplyRNA tissue kit (Promega, Alexandria, NSW) or RNeasy mini kit (Qiagen ...
-
bioRxiv - Cell Biology 2022Quote: ... TUNEL staining of the tissue sections was conducted using the DeadEnd Fluorometric TUNEL System (Promega), followed by immunofluorescence staining with DAPI ...
-
bioRxiv - Cell Biology 2019Quote: Genomic DNA (gDNA) was extracted using the ReliaPrep ™ gDNA Tissue Miniprep System kit (Promega) according to the manufacturer’s specifications ...
-
bioRxiv - Microbiology 2019Quote: Extracted vRNA from faecal and tissue samples were reverse transcribed using M-MLV RT (Promega) and random hexamer primers (Roche) ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from blood and tissue samples using the Maxwell®16 (Promega Corporation) instrument following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: RNA was purified from cells using the Maxwell RSC simply RNA tissue extraction kit (Promega) and the concentration was determined using a NanoDrop spectrophotometer (Thermo-Fisher) ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted with the Maxwell 16 FFPE Tissue LEV RNA Purification Kit (Promega, #AS1260). To avoid genomic DNA contamination ...
-
bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated with the Maxwell 16 FFPE Tissue LEV DNA Purification Kit (Promega, #AS1130). The double-strand DNA concentration (dsDNA ...
-
bioRxiv - Plant Biology 2024Quote: ... and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). The DNase treatment was performed according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated using the Maxwell 16 LEV simply RNA tissue kit (Promega, #AS1280), and RNA integrity was assessed using the Agilent 2100 Bioanalyzer ...
-
bioRxiv - Molecular Biology 2021Quote: ... IPs were performed by incubating lysates equivalent to 20 mg total protein supplemented with RNase inhibitor - RNasin (Promega, Germany, Cat. No. N2511) and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek ...
-
bioRxiv - Microbiology 2020Quote: ... cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
-
bioRxiv - Cancer Biology 2020Quote: In vitro translation of full-length SREBP1a and full-length SREBP1c was carried out using the TNT rabbit reticulocyte lysate translation kit (Promega, Wisconsin, USA) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
-
bioRxiv - Biochemistry 2021Quote: ... 20 μl of the cell lysate was used for luciferase reading using 50 μl of Renilla luciferase reagent (Promega, Madison, WI, USA). Relative light units (RLU ...
-
bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
-
bioRxiv - Immunology 2023Quote: ... 5% CO2 for 24 hrs, after which luciferase activity (Relative Light Units, RLU) was measured in cell lysates using the Bright-Glo system (Promega, Madison, WI) with a Bio-Tek II plate reader ...
-
bioRxiv - Microbiology 2021Quote: ... Total RNAs were isolated from the pellet using the Maxwell 16 LEV miRNA Tissue Kit (Promega) according to the manufacturer’s instructions and an extra TURBO DNase (Invitrogen ...
-
bioRxiv - Genomics 2021Quote: ... from freshly frozen brain tissues or the Wizard genomic DNA purification kit (Promega, Madison, WI, USA) from blood according to manufacturer instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: 100000 cells or 200 mg tissue were lysed in 100-500 μl Passive Lysis Buffer (Promega) for 15 min at room temperature on an orbital shaker ...
-
bioRxiv - Plant Biology 2021Quote: Total RNA extraction was performed with the ReliaPrep™ RNA Cell and Tissue Miniprep System (Promega), and cDNA synthesis was conducted with SuperScript™ IV Reverse Transcriptase (Invitrogen) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and RNA Tissue Miniprep System (Promega, UK, #Z6112 for tumor samples. GoScript™ (Promega, UK, #A5003) was used for the cDNA synthesis ...
-
bioRxiv - Microbiology 2021Quote: RNA was extracted using the Promega Maxwell 16 with the LEV simplyRNA tissue kit (Promega AS1270), the Roche MagNAPure with the Cellular Total RNA Large Volume kit (Roche 05467535001) ...
-
bioRxiv - Plant Biology 2021Quote: ... tissue was snap frozen and RNA isolated with the Maxwell® RSC Plant RNA kit (Promega). 1 µg of RNA was reverse transcribed into cDNA with the High-Capacity cDNA Reverse Transcription Kit with RNase inhibitor (Applied BiosystemsTM) ...
-
bioRxiv - Microbiology 2019Quote: Total RNA from colonic tissue samples was extracted using the EastepTM Total RNA Extraction Kit (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: RNA was extracted from root tip tissue with the Maxwell® RSC Plant RNA Kit (Promega) using the Maxwell® RSC instrument (Promega ...
-
bioRxiv - Microbiology 2019Quote: ... and remaining tissue incubated in 1X HBSS (without Ca2+ and Mg2+) containing 5 mM EDTA (Promega) and 1 mM DL-Dithiothreitol (DTT ...
-
bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: Total RNAs were extracted from microdissected tissues using the ReliaPrep™ RNA Cell Miniprep System (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2021Quote: DNA was extracted using the Promega AS1030 Maxwell 16 Tissue DNA Purification Kit (Promega, Wisconsin, USA) following the manufacturer’s protocols ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Total RNA extraction and DNase treatment were performed using the ReliaPrep RNA Tissue Miniprep System (Promega). RNA and DNA were quantified using a Qubit 2.0 fluorometer ...
-
bioRxiv - Molecular Biology 2022Quote: ... End-point PCR to detect gene expression in mosquito tissues was performed using GoTaq polymerase (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... The gDNA was isolated from selected clonal organoids using the gDNA tissue miniprep system (Promega, A2051) and evaluated by PCR and DNA gel electrophoresis using primer pair forward (5’-GCCCGTGGTCCAGCCTATC-3’ ...
-
bioRxiv - Microbiology 2023Quote: RNA was extracted from bacterial culture samples using Maxwell® 16 LEV miRNA Tissue Kit (Promega). cDNA was produced using random hexamers as primers and the GoScriptTM Reverse Transcription kit from Promega ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... then performed tissue lysis and DNA isolation using the Wizard SV Genomic DNA Purification kit (Promega). We fluorometrically quantified DNA using the PicoGreen dsDNA kit (Life Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... or the Maxwell® RSC miRNA from the Tissue and Plasma or Serum Kit (Promega, AS1680).
-
bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA from the LJ variants was isolated using Maxwell 16 Tissue DNA extraction kit (Promega) according to manufacturer’s instructions.
-
DNA Recognition and Induced Genome Modification by a Hydroxymethyl-γ Tail-Clamp Peptide Nucleic AcidbioRxiv - Genetics 2023Quote: ... genomic DNA (gDNA) was harvested using the Promega ReliaPrepTM gDNA Tissue Miniprep System (Promega, Madison, WI). Similarly ...
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted using the ReliaPrep RNA tissue miniprep system kit (Promega, Madison, WI, USA) and treated with RNase-free DNase according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... total RNA was isolated and DNAse I-treated using Maxwell® RSC simplyRNA Tissue kit (Promega), following manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
-
bioRxiv - Microbiology 2022Quote: ... Cells were then washed in PBS and infectivity was determined by measuring Renilla luciferase activity in the lysates of infected cells (Promega, Madison, WI, USA) using a Glomax luminometer (Promega).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The number of migrated cells was determined by measuring ATP in the lysates of transmigrated cells using a luminescence-based assay (CellTiter-Glo; Promega, Madison, WI, USA) and readouts were taken in the FlexStation 3 Multi-Mode Microplate Reader (Molecular de- vices LLC ...
-
bioRxiv - Systems Biology 2023Quote: ... Peptide loading was assessed by comparing the intensity of the total ion current to 200 ng of a human K562 whole cell lysate standard (Promega, Madison, Wisconsin, USA) prepared at 0.2 μg/μL.
-
bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and the cell lysates were prepared according to manual of the Dual-Luciferase® Reporter Assay System (Promega, Madison, USA) and measured with the GlowMax navigator microplate luminometer (Promega ...
-
bioRxiv - Cancer Biology 2023Quote: ... The β-Galactosidase activity was measured in 30 µl of lysate incubated with 20 µl of reporter lysis buffer and 50 µl of 2X β-gal Enzyme Assay buffer (Promega, Cat# E2000). The absorbance was read in iMark microplate reader (BioRad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... as well as pcDNA3.1-eGFP as a control were used as templates for in vitro transcription and translation using the T7 TNT® Coupled Reticulocyte Lysate System (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
-
bioRxiv - Biochemistry 2024Quote: ... The wild-type and mutated GCE variants were expressed in vitro using the TnT Quick T7 Coupled translation rabbit reticulocyte lysate system (Promega, Madison, WI, USA). The reaction products were examined in the dextran-coated charcoal binding assay4,9 with own synthesized [3H]JH III (25.7 Ci/mmol).62 The reticulocyte lysate without DNA input was the background control.
-
bioRxiv - Cell Biology 2020Quote: ... cell pellets were harvested and RNA extraction was performed with Maxwell LEV Simply RNA tissue kit (Promega) following manufacturer’s instructions with DNase I treatment ...