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Citations for Promega :
651 - 700 of 904 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Total bacterial RNA was extracted with Maxwell® 16 LEV miRNA Tissue Kit (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... 2 cm strips of intestinal tissues were embedded in low melting point agarose (Promega) to enrich for well-oriented crypt-villus units ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA isolation was performed using ReliaPrep™ RNA Tissue Miniprep System (Promega Corporation) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Nluc expression in tissue culture supernatants was quantified using Nano-Glo luciferase substrate (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2020Quote: ... Extracted tissues (10 animal caps or ventral halves) were transferred into lysis buffer (Promega) and incubated for 15 min ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated using the Maxwell 16 LEV simplyRNA Tissue Kit (Promega, AS1280) and quantified by Qubit® RNA BR Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted using a TRIzol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). Reverse transcription was carried out with PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was isolated with the Maxwell 16 DNA Tissue DNA Purification Kit (AS1030, Promega) following the manufacturer’s protocal ...
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bioRxiv - Cancer Biology 2023Quote: ... and cells using Maxwell 16 LEV Simply RNA Tissue Kit (Promega, Madison, WI, USA), according manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μm tissue scrolls were processed using the Maxwell RSC RNA FFPE instrument (Promega). RNA concentration and quality was determined using a TapeStation automated electrophoresis instrument (Agilent) ...
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bioRxiv - Immunology 2023Quote: ... Tissues were permeabilized and blocked in blocking buffer with 0.05% Tween 20 (H5152, Promega) and 5% BSA (A3059 ...
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bioRxiv - Cell Biology 2024Quote: ... L929 cell lines and intestinal tissue sections was determined by TUNEL staining (G3250, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... as well as Tagged-BAF15 were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: hNAA40 and dTIS mutagenized hNAA40 pOTB7 constructs were used as templates for in vitro coupled transcription/translation in a rabbit reticulocyte lysate system according to the manufacturer’s instructions (Promega, Madison, WI) to generate [35S] methionine labeled translation products ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation reactions were carried out with 5 ul of either supplemented rabbit reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of cell lysate was distributed to mix with 25 μL of Nano-Glo Luciferase Assay System (Promega, Cat.# N1120), and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Systems Biology 2019Quote: ... lysates were diluted in 700µl of TBS and incubated at 4°C with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the A-MLV titers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... transduction efficiency was quantified by measuring the activity of firefly luciferase in cell lysates using a luciferase assay system (Promega, USA) and SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was assessed by quantifying luciferase activity in cell lysates using a commercial kit (Luciferase Assay System, Promega, Cat. #E1500) and a plate-reading luminometer (SpectraMax i3x ...
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Microbiology 2021Quote: ... for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega, E1501).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the VSV-G titers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cellular lysates were assayed for luciferase and Renilla activities using the Dual-Luciferase Reporter assay system (E1980, Promega, Madison, WI, USA). Luminescence was measured using Glomax 20/20 luminometer (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega, Cat.# E6130). The plate was placed on a shaker for 5 min in the dark ...
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bioRxiv - Molecular Biology 2022Quote: Tethering assay in rabbit reticulocyte lysates was performed by applying in vitro transcribed and capped mRNA encoding mApple-YTHDF1N-MCP or mApple-MCP to the lysates (Promega, L4960) for effector protein translation ...
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bioRxiv - Cell Biology 2022Quote: The other in vitro translation was done in the presence of radioactive [35S]methionine using the TNT T7 Reticulocyte Lysate System (Promega 14610). In vitro–translated proteins were then precleared by incubation with empty glutathione Sepharose beads in NETN buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates were prepared as per the manufacturers protocol using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega: G7570). Briefly ...
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bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were generated with pCS2-N-terminal 5×MYC vectors and pCS2-N-terminal 3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications ...
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bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Neuroscience 2023Quote: mRNAs of C9orf72 intron1-(G4C2)80 with a Myc tag in the GA frame were in vitro translated with Flexi® Rabbit Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from cells using the ReliaPrep™ tissue system kit (Promega; Z6012). cDNA was produced from 1 μg of RNA sample using M-MLV reverse transcriptase (Promega ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from mouse hearts using ReliaPrep™ RNA Tissue miniprep system (Promega). cDNA was synthesized using M-MLV Reverse Transcriptase (Promega) ...
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bioRxiv - Genomics 2020Quote: ... total RNA was isolated using the automated Maxwell LEV 16 simplyRNA tissue kit (AS1280, Promega). cDNA was synthetized from 500 ng total RNA using Superscript III (18080085 ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was extracted from FFPE tumor tissue using the Maxwell RSC DNA FFPE Kit (Promega) or the GeneRead DNA FFPE kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... The total RNA was extracted using the Maxwell® LEV Simply RNA Tissue kit (Promega) or the RNeasy Plant Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... flash frozen and RNA was extracted using the ReliaPrep™ RNA Tissue Miniprep System (Promega) according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2022Quote: Extracted DNA was obtained from tissue biopsies using the Maxwell 16 DNA extraction kit (Promega). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Boston Children’s Hospital used the Maxwell RSC Tissue DNA Kit for extraction (Promega, Madison, WI) and the Quantus Fluorometer and Picogreen (Promega ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted using the Promega Maxwell 16 with LEV simplyRNA Tissue Kits (Promega AS1270). RNA quality was assessed using the Agilent Bioanalyzer and appropriate samples were used for NGS library preparation with the NEBNext Ultra II Directional RNA kit (NEB E7760 ...
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bioRxiv - Microbiology 2021Quote: ... total RNA was isolated using ReliaPrep™ RNA Tissue Miniprep System (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...