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Citations for Promega :
6001 - 6050 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... U87MG cells were authenticated within a year of their use by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega). Unless otherwise indicated ...
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bioRxiv - Microbiology 2023Quote: Genomic DNAs were purified from 600 µL of overnight bacterial culture using the Wizard HWM genomic DNA prep kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from backup samples for transcriptome analysis using Eastep® Super Total RNA Extraction Kit (Promega, USA). Reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality was assessed with an Agilent Bioanalyser and the quantity was calculated by an RNA-specific kit from Quantus (Promega).
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Microbiology 2023Quote: High-molecular weight genomic DNA was isolated based on a modified version of Promega’s Genomic DNA Isolation Kit (Promega, A1120) [63] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA from feather pulps and tracheas were extracted using the MagaZorb® DNA mini-prep kit (Promega, Madison, WI), following the manufacturer’s recommendations with a few modiications ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria were pelleted by centrifugation and DNA extraction was performed with the Wizard® genomic DNA purification kit (Promega).
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bioRxiv - Developmental Biology 2024Quote: Substrate titration kinase reactions were performed on the indicated recombinant LIN-42 proteins (CK1BD + Tail WT or mutants) using the ADP-Glo kinase assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... HBEC cells were incubated for an additional 24 hours and then luciferase activity was measured using the Dual-Luciferase Reporter assay kit (Promega) on a Glomax instrument (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: ... The 4 µg of linearized vectors were transcribed 5h using RiboMAX™ Large Scale T7 RNA polymerase kit (Promega, #P1300) with 5.6 mM Ribo m7G Cap Analog (Promega ...
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bioRxiv - Microbiology 2024Quote: The levels of glutathione (GSH) of GECs were determined using a luminescence-based GSH/GSSG-Glo Assay kit (Promega, V6611) [41] ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and used the GoScript Reverse Transcription System kit to generate cDNA from extracted RNA (Catalog #A5000, Promega Corporation, Madison, WI). We designed primers using the tool primer-Blast134 and the predicted cDNA sequence of kitlga (Fig ...
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bioRxiv - Plant Biology 2024Quote: One microgram of DNA-free total RNA was reverse transcribed using Avian Myeloblastosis Virus (AMV) reverse transcriptase and oligo(dT)_15 primers (Reverse Transcription System Kit, Promega). PCR amplification was performed with Phusion DNA Polymerase (LifeTechnologies ...
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The myokine FGF21 associates with enhanced survival in ALS and mitigates stress-induced cytotoxicitybioRxiv - Neuroscience 2024Quote: ... following the manufacturer’s instructions and as previously described.25 Apoptosis was detected by caspase activity using Caspase-Glo 3/7 assay kit (Promega) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: A specific double-stranded RNA (dsRNA) was synthesized using the T7 RiboMAX Express RNAi System kit (Promega, Madison, WI, USA), and the primer sequence was listed in Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... Cytotoxicity was measured by quantifying the activity of lactate dehydrogenase (LDH) in the extracellular medium (Cytotox 96 non-radioactive cytotoxicity kit, Promega) and normalised against cells lysed by 0.04% Triton X-100 ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were then assayed for IL-1β (human; DuoSet; R&D) and relative LDH levels (as a surrogate for pyroptosis) using a CytoTox 96 Kit (Promega). For the THP-1 LPS signaling assays ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were then transferred to a 96-well plate for storage and assayed for IL-1β (human; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). Once supernatants were removed ...
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bioRxiv - Microbiology 2024Quote: ... and a dual luciferase assay was conducted using the Dual-Glo® Luciferase assay kit according to standard protocol (Promega). Luminescence was measured using a luminometer (GloMax®-Multi Detection System ...
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bioRxiv - Microbiology 2024Quote: ... using random primers for cDNA synthesis and gene-specific primers (primer sequences are listed in S3 table) for qPCR (GoTaq qPCR kit, Promega). The transcript levels for innate immune factors were normalized to the geometric mean of those for the housekeeping genes ACTB and HPRT (ΔCT ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from different tissues of shrimp using the Eastep Super Total RNA Extraction Kit (Promega, Shanghai, China). The genomic DNA of shrimp tissues was extracted using a genomic DNA extraction kit (Vazyme ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was genotyped for 15 autosomal STR loci and amelogenin (X/Y) with the Powerplex 16HS PCR kit (Promega). Positive control (gDNA 2800M ...
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bioRxiv - Microbiology 2024Quote: SAM-dependant N6mA DNA methylation activity of PglX was probed in vitro using an MTase-Glo Methytransferase Assay kit (Promega). The kit allows indirect measurement of SAM-dependent methyltransferase activity via production of the SAH reaction product ...
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bioRxiv - Developmental Biology 2024Quote: The efficacy of all MOs was verified in western blots following in vitro transcription and translation (TNT-coupled Wheat Germ Extract kit, Promega) of pCMV-Sport6-marcks.S ...
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bioRxiv - Systems Biology 2024Quote: ... Extracts were used for quantification of luciferase as proxy for ATP content using the ENLITEN ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... In a 96 well plate (white bottom) 20 µL lysate was mixed with 50 µL Luciferase Assay Reagent (#E1501 kit, Promega) (as per the manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2024Quote: ... Macroscopic dissection of tumor areas was performed with sterile scalpels and tissues were extracted using the Maxwell RSC FFPE Plus DNA Purification Kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was then reverse transcribed into complementary DNA (cDNA) utilizing the Improm II Reverse Transcriptase kit (Promega, Madison, WI, USA). Quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Biochemistry 2024Quote: Kinase reactions were performed on the recombinant human PER2 FASP or Degron using the ADP-Glo kinase assay kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA (gDNA) from male naïve ESCs and extended EpiLCs was extracted using the Wizard Genomic DNA Purification Kit (Promega A1120), following the instructions for Tissue Culture Cells ...
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bioRxiv - Microbiology 2024Quote: EMCV molecular clones were linearised with BamHI and genomic RNA was in vitro transcribed using the T7 RiboMax kit (Promega). Transcripts were purified (RNeasy kit ...
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bioRxiv - Immunology 2024Quote: ... Supernatants are then transferred to a fresh 96-well plate for storage and assayed for IL-1β (canine, monkey, porcine, rabbit or rat; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). Once supernatants are removed ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and subsequently lysed with 5X passive lysis buffer supplied with the luciferase assay kit (Promega). Following a brief vortex ...
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bioRxiv - Genetics 2024Quote: ... including the two strains used for the mapping studies was isolated from 20 females (2 × 10 flies/prep) from each line using the Maxwell 16 Tissue DNA purification kit on the Maxwell 16 instrument (Promega). Samples were eluted in 300 μl water containing RNase A at 20 mg/ml ...
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bioRxiv - Systems Biology 2024Quote: ... The transfected cells were treated with 5 ng/ml TGFβ1 or vehicle for 15 min and Luciferase activities were measured using the Dual-Luciferase Reporter Assay Kit (E1910; Promega).
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bioRxiv - Cancer Biology 2024Quote: ... or in-house synthesized tracers according to the manufacture’s protocol (NanoBRET™ TE Intracellular Kinase Assay Kit, Catalogue # N2521, Promega).
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bioRxiv - Biochemistry 2024Quote: 20merRBNS and posRBNS DNA pool was synthesized by IDT (Supp. Table 4) and was in vitro transcribed using T7 RiboMAX Express large scale RNA kit (Promega) according to manufacturer protocols.
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bioRxiv - Cell Biology 2024Quote: ... Nanoluciferase levels in fecal samples were tracked as a proxy for parasite burden as previously described50 using a NanoGlo Luciferase kit (Promega).
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bioRxiv - Evolutionary Biology 2024Quote: ... on the 12th day (n=3 individuals/ dietary treatment/sex) and isolated microbial DNA using a Wizard genomic DNA isolation kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA was extracted from the samples with the Promega Wizard™ Genomic DNA Purification Kit (Promega, Madison, WI, USA), according to the salt-based extraction protocol of Sambrook and Russel (2001) ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity of cell lysate was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax Discover System ...
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bioRxiv - Synthetic Biology 2021Quote: ... The luciferase activity in the cell lysates was measured using a GloMax Navigator system (Promega). All luciferase assays were conducted in LumiNunc 96-well white plates (Thermo Scientific ...