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Citations for Promega :
5901 - 5950 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... we measured the level of accumulated 2-deoxyglucose-6-phosphate (2DG6P) using the assay kit according to the manufacturer’s protocol using a GloMax®96 microplate luminometer (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured at 550 to 570 nm wavelength and followed by 100 μl of 1X Stop & Glo Reagent (part of the kit E1910, Promega) was added to each well ...
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bioRxiv - Genetics 2021Quote: ... all clippings from both hands and feet were used to extract genomic DNA with DNA IQ Casework Pro Kit (#AS1240, Promega) and Tissue and Hair Extraction Kit (#DC6740 ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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bioRxiv - Microbiology 2021Quote: ... LucNeo#2 cells were treated with the compounds indicated in Figure 3C for 48 h and the luciferase activity was measured with Luciferase Assay System kit (Promega). Sofosbuvir ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from a 300 μL plasma aliquot using the Viral Total Nucleic Acid Kit (Promega, Madison, WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed at 36 h after transfection and the luciferase activity was analyzed using the Dual-Luciferase Reporter Assay Kit (Promega).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was extracted from a 300 μL plasma aliquot using the Viral Total Nucleic Acid Kit (Promega. Madison. WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from each isolate to be sequenced was purified using the Wizard Genomic DNA Purification Kit (Promega, Fitchburg, WI) and libraries were constructed and indexed using NexteraRTM DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from about 35 mg of tissue using the Maxwell™ 16 Tissue DNA purification kit (Promega) and total DNA concentrations were normalized to 20 ng/µl ...
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bioRxiv - Molecular Biology 2021Quote: ... pancreatic sections were submitted to terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay using a DeadEnd Fluorometric TUNEL system kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Media was removed and cells were washed in PBS before re-suspension in 75 μL 1X Passive Lysis Buffer (PLB; Dual-Luciferase Reporter Kit, Promega). Cells were incubated in PLB for 15 min at RT and further homogenised by pipetting ...
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bioRxiv - Immunology 2020Quote: ... The plate reader was programmed to inject 40 μl of Luciferase Assay Substrate re-suspended in Luciferase Assay Buffer II and 35 μl of 1X Stop&Glo Reagent (Dual-Luciferase Reporter Kit, Promega) per well ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a DNase I treatment and a subsequent reverse transcription with oligo d[T]18 using the GoScript RT kit (Promega). One-step RT-qPCR was performed using the SYBR FAST Mix optimized for LightCycler 480 (KAPA ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...
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bioRxiv - Genomics 2021Quote: Total genomic DNA of bacterial isolates was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Tris(2-carboxyethyl)phosphine (TCEP) and BCA protein assay kit were from Pierce and sequence-grade trypsin was from Promega. Rapigest and Sep-Pak C18 columns were from Waters and C18 Zip tips were from Millipore ...
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bioRxiv - Immunology 2022Quote: ... Cells were immediately stimulated with cytokine for 24 hr and nanoluciferase activity was measured relative to control activity using the NanoGlo Dual Luciferase kit (Promega). The primers for cloning enhancer fragments into pNL3.1 are in Table S3.
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bioRxiv - Immunology 2023Quote: ... total RNA was purified from 3-4 tissues of female flies using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega ...
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bioRxiv - Microbiology 2022Quote: ... All VLP volumes were calculated and normalized to 1.4 x 107 RLU using the Nano-Glo® HiBiT Lytic Detection System kit (Promega).
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bioRxiv - Immunology 2023Quote: ... Luciferase and Renilla signals were measured 48 h after transfection by a Dual-Luciferase Reporter Assay Kit (Promega, No. E1980). Data were analyzed and normalized by the division of firefly luciferase activity with that of Renilla luciferase to eliminate transfection efficiency difference.
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bioRxiv - Immunology 2023Quote: ... viral RNA (vRNA) was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison WI). Next ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (2000 g x 5 min) and subjected to genomic DNA (gDNA) extraction using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... higginsianum genomic DNA used as PCR templates were isolated by Maxwell RSC Plant DNA Kit with Maxwell RSC 48 instrument (Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Plant Biology 2023Quote: ... Infiltrated leaves were harvested 48 hrs after infiltration and luciferase activity was measured immediately using the Dual-Glo Luciferase Assay kit (Promega). Leaf disks 4 mm in diameter were cut and inserted into white 96 well plates with 75 µl of 1x PBS and 75 µl of luciferase assay reagent ...
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bioRxiv - Plant Biology 2023Quote: ... Five micrograms of total RNA were ligated to the RNA GeneRacer oligo adapter and subjected to reverse transcription utilizing Improm-II Reverse Transcriptase kit (Promega). The cDNA was used for amplification of cleaved PYL6 fragments ...
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bioRxiv - Genetics 2023Quote: ... After a second purification using a Zymo DNA Clean and Concentrator kit the PCR product was used as a template in a T7 RiboMAX express RNAi System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
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bioRxiv - Molecular Biology 2023Quote: ... The ratios of luciferase activity for the renilla and firefly luciferases were measured 48 hrs (hours) after transfection using the dual- Luciferase reporter assay kit from Promega and a luminometer ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was isolated from overnight cultures (5 mL) using the Wizard Genomic DNA Purification Kit (Promega GmbH, Walldorf) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: Genomic extraction for the WT C6706:str2 strain and each of the resistant mutants was performed using Wizard Genomic DNA Purification Kit (Promega). The concentration of DNA was quantified using Nanodrop spectrophotometer (ND-1000) ...
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bioRxiv - Zoology 2023Quote: Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit with the Maxwell 16 Research Instrument (Promega, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial cells were collected from 2mL of the culture and the genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Samples were then sent to Novogene Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... Phenol-chloroform phase separation and chloroform phase separation steps were repeated to remove the RNase and then DNA was purified from the aqueous phase by using Promega kit protocol (Promega Wizard SV Gel and PCR Clean-Up System ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and used as template to generate dsRNA in vitro using the Promega T7 RibomaxTM Express RNAi System kit (Promega, P1320). The dsRNA was precipitated with sodium acetate (300 mM final ...
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bioRxiv - Immunology 2024Quote: ... Lactate in flow-through medium was quantified using the L-lactate assay kit (Biomedical Research Service & Clinical Application, #A-108) and GloMax microplate reader (Promega) as suggested by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: Glucose uptake by cultured primary astrocytes was studied using a bioluminescent method with the Glucose Uptake Assay Kit (Promega, J1341) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were passaged every 3–4 days and regularly checked for mycoplasma infections using a GoTaq G2 Hot Start Taq Polymerase kit from Promega.
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bioRxiv - Microbiology 2022Quote: In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations ...
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bioRxiv - Biochemistry 2024Quote: Kinase activity was measured in a generic kinase reaction buffer (20 mM Tris pH 7.4, 50 mM KCl, 10 mM MgCl2) using the ADP-Glo assay kit (Promega, V6930) and on a commercial CK2 substrate peptide (EMD Millipore ...
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bioRxiv - Microbiology 2023Quote: ... The inoculum was added to HEK293T cells and incubated at 37 ° C with 5% CO2 and the luciferase signal was with analyzed using luciferase assay kit (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was transferred into a fresh tube and used to measure luciferase activity with Dual-Luciferase Reporter Assay kit (Promega) on a Lumat LB9507 luminometer (Berthold Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... the 1X Renilla-luciferase substrate mixed with a Stop & Glo buffer (100 µL/well, Promega Dual Glo kit, cat # E1960) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...