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Citations for Promega :
5801 - 5850 of 8253 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Preserved tissue (10-15 mg) was used for nucleic acid purification by employing the Wizard genomic DNA kit (Promega, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1 µg RNA was used to synthesize the oligo(dT) primed first-strand cDNA using the M-MLV reverse transcriptase cDNA synthesis kit (Promega). Quantitative RT-PCR was performed using a CFX96 Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... and the relative rDNA promoter activity was measured in cell lysates using a dual luciferase assay kit (https://worldwide.promega.com/products/luciferase-assays/reporter-assays/dual_luciferase-reporter-assay-system/?catNum=E1910, Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Genomics 2020Quote: ... of blood sample was collected from each individual and genomic DNA was extracted by using Maxwell RSC whole blood DNA extraction kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted from selected clones as well as in the parental clone using the Wizard Genomic DNA Purification Kit (Promega). NGS was performed using Nextera XT DNA Library Preparation kit and the NextSeq 500 sequencing systems (Illumina ...
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bioRxiv - Genomics 2020Quote: ... After 48 h Luciferase assay was performed according to manufacturers’ instruction using the Dual-Luciferase Reporter Assay System Kit (Promega).
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bioRxiv - Genomics 2020Quote: The total RNA was extracted from Inner Mongolia Alashan cashmere goats and Dazu black goats using RNA extraction Kit (Promega), and RNA quality and concentration were measured on an Agilent 2100 Bioanalyzer (Germany) ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: Genomic RNA was extracted from small intestine and large intestine samples using the SV Total RNA Isolation kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior of the sponge tissue (<2mm from the exterior pinacoderm) and the seawater filter papers using the Wizard Genomic DNA Purification Kit (Promega).
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bioRxiv - Plant Biology 2020Quote: ... only root parts (around 100 mg) were collected and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). For verifying the transcript level in single T-DNA lines ...
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bioRxiv - Genetics 2021Quote: ... The genomic DNA (gDNA) of individual transient G0 mosquitoes was extracted using the Wizard® Genomic DNA Purification Kit (Promega). In the T7 Endonuclease I (T7EI ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox1 Kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Microbiology 2021Quote: ... The luciferase expression levels were measured 24h post-transfection using a Nano-Glo Dual-Luciferase Reporter Assay kit (Catalogue number N1610, Promega) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Caspase 3/7 and 9 activities were assessed using a fluorescence-based Apo-ONE homogenous caspase 3/7 assay kit (Promega) and luminescence-based caspase-glo 9 assay system (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: ... grown for 48 h and then caspase 3/7 enzymatic activity determined using Apo-One Homogenous caspase 3/7 activity assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Luciferase activity was tested 24 hours after plasmid transfections using the Dual-Glo Luciferase Assay System kit (Cat# E2920, Promega) as per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from nasopharyngeal swab samples using Maxwell® 16 Viral Total Nucleic Acid Purification Kit (Promega, Wisconsin-USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total mRNAs used for qRT□PCR experiments were isolated from Chlamydomonas using the Eastep Super total RNA extraction kit (Promega), and cDNA was synthesized with the PrimeScript TMRT Master Mix kit (TaKaRa) ...
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bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... were collected from each genotype and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). Reverse transcription was carried out with PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Neuroscience 2020Quote: ... Cell viability was also determined based on lactate dehydrogenase (LDH) activity in conditioned culture media using a commercial kit (G1780, Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was also extracted from inactivated grown cultures using a DNA extraction kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Microbiology 2021Quote: Epithelial cytotoxicity was determined by quantification of lactate dehydrogenase activity in A549 culture supernatants at 24 h post fungal challenge using the Cytox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... MT4 and TMZR5 cells were transduced with the genes in Fig 2G as before and 96 hours post-transduction the supernatants were harvested to measure toxicity of the expressed ISGs using the CytoTox-Glow kit (Promega). In a separate experiment ...
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bioRxiv - Microbiology 2020Quote: gDNA was extracted from stationary phase evolved cocultures following revival from frozen stocks using a Wizard Genomic DNA purification Kit (Promega). DNA fragment libraries were constructed for samples from shaking WT-based cocultures A-F and NifA*-based cocultures M-R at generation ∼146 using NextFlex Bioo Rapid DNA kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase3/7 activity was measured 3 days after the treatment with a commercial available kit (Caspase-Glo 3/7 Assay, Promega).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded in 96 wells plate and luciferase activities were measured using Dual-Luciferase Reporter Assay System kit as described by the manufacture (Promega). Fluc/Rluc ratios were determined as relative luciferase activities.
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bioRxiv - Cell Biology 2021Quote: ... The RNA concentrations were measured and 1ug RNAs were used to synthesize cDNA using the GoScript Reverse Transcription System kit(Promega).
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bioRxiv - Cancer Biology 2021Quote: ... cells using ITX2 transfection reagent and luciferase activity (renilla and firefly) was measured 24hours later with the Dual Glow-Stop and Glow luciferase kit (Promega) using a BioTek luminometer (Synergy).
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bioRxiv - Cancer Biology 2021Quote: ... The desired PCR fragments were isolated from a 1% agarose gel and purified using the Wizard Genomic DNA Purification Kit (Promega). In order to identify the specific edition ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... PCR products were isolated with a QIAquick Gel Extraction kit (Quiagen) and purified fragments cloned into pGEM-T Easy vector (Promega). Specific details for the generated probes are as follow (gene name (accession number) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were loaded with 5 μM CellROX in media for 30 min and loaded and unloaded control cells analysed for mCherry channel signal using flow cytometry as described above GSH and GSSG levels were measured using GSH/GSSG-Glo assay kit from Promega according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The SELENOP 3’UTR and the control plasmid were linearized using Bsb 1 and in vitro transcribed using the Ribomax kit (Promega). The RNA was then purified using p30 size exclusion columns (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... DNA extractions were carried out using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, United States of America) following Lehner et al ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Plant Biology 2022Quote: ... Samples representing different stages of the parasitizing process (i.e. root development) were extracted using the Maxwell® RSC Plant RNA kit (Promega) with the Maxwell® RSC Instrument (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity (Relative Luciferase Units or RLU) was detected at 72h post-infection by using Bright-Glo Luciferase Assay System Kit (Promega) in a Microplate Luminometer (Wallac-Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity (Relative Luciferase Units or RLU) was detected at 72 h post-infection by using Bright-Glo Luciferase Assay System Kit (Promega) in a Microplate Luminometer (Wallac-Perkin Elmer).
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bioRxiv - Biochemistry 2022Quote: ... SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega). For gRNAs utilized in in vitro cleavage reactions containing SpRY from human cell lysates ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were incubated for approximately 18-20 hours and luciferase was measured using the Promega BrightGlo luciferase kit following manufacturers’ instructions (Promega). Entry signal was normalized to the average signal for the “no spike” control ...
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bioRxiv - Biochemistry 2022Quote: The luciferase assay described in this work is based on the luciferase assay system kit protocol from Promega (WI, USA) which was performed to monitor the downstream transcription activation potential of the native and mutated MopR.68 The overnight grown cultures of E.coli DH5α cells harboring the recombinant MopRLUC and MopRG148PLUC plasmids were sub-cultured and grown to the log phase till the OD at 600 nm reached ∼0.3 ...
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bioRxiv - Microbiology 2022Quote: The total RNA of tissues was extracted using the Maxwell RSC simply RNA tissue kit (AS1340, Promega, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... HCT116 cell line was treated for 24 h with 10 µM curcumin and RNA was isolated using the Maxwell® 16 IVD system and the Maxwell Simply RNA Tissue Kit according to the manufacturer’s instructions (Promega). RNA was reverse transcribed using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: The genomic DNA of the 5 assessed M strains were isolated and purified using the Wizard Genomic DNA Purification Kit (Promega). The growing of strains ...
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bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity was measured using a luminescence Caspase Glo 3/7 assay kit (G8090; Promega, Madison, WI, USA) according to the manufacturer’s instructions ...