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Citations for Promega :
551 - 600 of 1320 citations for Rat NDUFS4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... The PCR product was cloned into the pGL3-promoter luciferase-based plasmid (Promega) at the cloning site between KpnI and XhoI ...
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bioRxiv - Immunology 2019Quote: ... Plasmids were transfected into RAW264.7 cells with FuGENE HD (Promega, Madison, WI, USA). At 50 % confluency ...
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bioRxiv - Microbiology 2019Quote: ... DNA restriction nucleases were used to digest plasmids (Promega and New England Biolabs). Gibson assembly mixes were used according to manufacturer instructions to construct the plasmids used in this study (New England Biolabs) ...
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bioRxiv - Physiology 2019Quote: ... Transfection of plasmids or siRNAs was performed with FuGene HD (Promega, Madison, WI) or Lipofectamine RNAimax (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... ISRE-firefly luciferase and pTK-renilla luciferase (pRL-TK) plasmids were from Promega. Vectors expressing MAVS ...
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bioRxiv - Biochemistry 2020Quote: A549 cells were transiently transfected with the plasmid pGL4.37[luc2P/ARE/Hygro] (Promega) and selected with 500 μg/ml of hygromycin to obtain a stable cell line harboring the ARE-luc2P reporter ...
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bioRxiv - Cell Biology 2021Quote: DNA plasmid transfections were performed using Fugene HD transfection reagent (Promega Corporation, E2311) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... 100 ng/well indicated plasmids and 10 ng pRLTK internal control vector (Promega) were co-transfected with 1.25 μl Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... These fusions were expressed from plasmids based on the pGEM-T backbone (Promega) named pOP plasmids (22) ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmids were diluted in Opti-MEM and Fugene HD transfection reagent (Promega). The DNA-mixture was incubated for 30 min and then added drop-wise to the cells cultured in DMEM without penicillin/streptomycin for 6-7 h ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmids were diluted in Opti-MEM and Fugene HD transfection reagent (Promega). The DNA-mixture was incubated for 30 min and then added drop-wise to the cells cultured in DMEM without penicillin/streptomycin for 6-7 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and a Renilla luciferase reporter plasmid driven by a constitutive CMV promoter (Promega). 24 hours later ...
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bioRxiv - Microbiology 2022Quote: Plasmids (Table S2) used were constructed with DNA restriction nucleases (Promega – WI, USA), Gibson Assembly mix (New England Biolabs – MA ...
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bioRxiv - Immunology 2022Quote: ... Plasmids were transfected into cells with the aid of FugeneHD (Promega, Cat. E2311) using a ratio of 4 uL FugeneHD per 1 ug of plasmid DNA ...
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bioRxiv - Cell Biology 2022Quote: Transfection of plasmid DNA in HeLa cells was carried out using FuGENE (Promega). For the DNA transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid purifications were performed utilising Wizard Plus SV Minipreps DNA Purification Systems (Promega). New plasmids were tested by restriction enzyme digests and sequencing (Eurofins/GATC or Microsynth) ...
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bioRxiv - Microbiology 2020Quote: ... Fluc was amplified from pGL4.32[luc2P NF-kB-RE] plasmid (Promega, Madison, WI) and inserted into the vector backbone using Gibson Assembly ...
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bioRxiv - Genetics 2022Quote: ... HEK cells were transfected with 500 ng of plasmid using FuGENE 6 (Promega) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: DNA plasmid transfection were performed as previously described using FugeneHD (Promega, Madison, WI) 51 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... This genomic region had previously been cloned into the pGEM-T plasmid (Promega) and transformed into Escherichia coli(Ye et al ...
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bioRxiv - Neuroscience 2019Quote: ... and pGlosensorTM -22F cyclic adenosine monophosphate (cAMP) biosensor plasmid (Promega Corp., Madison, WI), which were used for receptor activation and intracellular signalling assays.
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid DNA (6 μg) was delivered into U2OS cells using FuGENE 6 (Promega) and 2.5 μg of plasmid DNA was delivered into HeLa cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The coding sequence of BclA3CTD was cloned in the plasmid pGEMT-easy (Promega) and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: Plasmids were transfected into U2OS cells using FuGENE® HD Transfection Reagent (Promega), according to the manufacture’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Oligos were annealed and inserted into the NotI/XhoI-digested psiCHECK2 plasmid (Promega). 1E5 cells/well were plated in 48-well plates and transfected with empty ...
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bioRxiv - Genomics 2019Quote: Linearized plasmid (2.5 ug) was added to a mixture containing transcription buffer (Promega), 10 mM DTT ...
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bioRxiv - Biophysics 2019Quote: ... The plasmids were transformed in Rosetta (DE3) and Rosetta (DE3) pLys cells (Promega). Cells were grown to an optical density of 0.7-0.8 in Luria-Bertani (LB ...
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bioRxiv - Genetics 2021Quote: ... Plasmid and template were co-transfected into LF2 mESCs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 0.02 ug of the pRL-CMV Renilla Luciferase plasmid (Promega Accession No. AF025843) was co-transfected with firefly luciferase plasmids ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual colonies were selected and isolated using the PureYield Plasmid Miniprep System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... where Firefly luciferase was substituted in the plasmid for NanoLuc luciferase (Promega, USA), transfected to HEK293 cells and IFNα-luciferase fusion proteins collected with tissue culture supernatant ...
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bioRxiv - Genomics 2019Quote: ... 400ng of the construct was transfected together with 40ng of Renilla plasmid (Promega) using lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... and target (400 ng) plasmids using 6 μL FuGene HD (Promega, Cat# E2311). 72 hrs later ...
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bioRxiv - Microbiology 2022Quote: ... The extracted plasmid was linearized with restriction enzyme BamHI (Promega, Madison, WI, USA), and thereafter purified with QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was purified using the Pure Yield Plasmid Midi Prep System (Promega, A2495).
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bioRxiv - Evolutionary Biology 2022Quote: Plasmid and B2 insert were ligated together using a rapid ligation kit (Promega). Ligation reactions contained 2.5µl 4x buffer ...
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bioRxiv - Microbiology 2022Quote: ... Replicon RNA was transcribed from linearized template plasmids with T7 RiboMAX Express (Promega) and cleaned by MEGAclear (Ambion/ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
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bioRxiv - Biophysics 2022Quote: ... transient transfection of the appropriate plasmid(s) was performed using FuGENE HD (Promega) according to the manufacturer’s procedure ...
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bioRxiv - Microbiology 2023Quote: ... The presence of the plasmid was confirmed by colony PCR (GoTaq polymerase, Promega). For construction of ATCC17978Δhcp::aprR ...
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bioRxiv - Biochemistry 2023Quote: ... purified using the PureYield Plasmid Miniprep or Midiprep Systems (Promega # A1222 and A2495), and validated by Sanger sequencing at The Ohio State University Comprehensive Cancer Center Genomics Shared Resource (OSUCCC GSR) ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transfected into HEK293T cells using the ViaFect™ Transfection Reagent (Promega) according to manufacturer instructions 45.
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bioRxiv - Cancer Biology 2023Quote: ... Transfections were performed by complexing plasmids with FuGENE® 6 Transfection Reagent (Promega) in OptiMEM media (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and pDEST26-Renilla plasmid (0.1 μg) using FuGENE® HD (Promega, Cat# E2312). Twenty-four hours after transfection ...
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bioRxiv - Genomics 2023Quote: ... The guide and donor plasmids were transfected into cells using Fugene HD (Promega). After seven days ...
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bioRxiv - Microbiology 2023Quote: ... The pRL-TK Renilla luciferase reporter plasmid was purchased from Promega (Madison, WI). E ...
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bioRxiv - Biochemistry 2023Quote: ... DNA used for transfection was purified using the PureYield Plasmid Midiprep System (Promega). After incubation for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid transfections were performed in MDA-T32 cell line using Fugene 6 (Promega). Transfected cells were cultured for over 3 weeks in complete RPMI-1640 medium containing G418 (600 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... The firefly luciferase expression plasmid pGL3-CMV was purchased from Promega (Madison, WI).
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...