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Citations for Promega :
351 - 400 of 1320 citations for Rat NDUFS4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... together with pRL-CMV reference plasmid (0.03 µg/well; Promega). Cells were simultaneously co-transfected with 0.3 µg/well of the empty pCI-neo-3xFLAG expression vector or encoding viral proteins as specified ...
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bioRxiv - Microbiology 2019Quote: ... Amplified plasmid was then phosphorylated using T4 polynucleotide kinase (Promega) and self-ligated (T4 DNA ligase ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The sequence was subcloned into the pCMVTNT expression plasmid (Promega). Other plasmids used in this study express X ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with plasmids of interest using Fugene (Promega) as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCR products were cloned into pGEM®-T plasmid (Promega) and transformed into TOP10 chemically competent E ...
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bioRxiv - Microbiology 2020Quote: ... as carrier DNA and a luciferase-encoding plasmid (pGL4.26, Promega). qPCR was then performed to detect viral gene ORF26 and luciferase from the carrier plasmid using GoTaq polymerase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293FT cells were transfected with the plasmid using FuGENE6 (Promega). After 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 ng pGL3-Basic luciferase reporter plasmid (Promega, Cat. #E1751) with target sequence inserts were cotransfected into HEK293T and MCF7 cell lines with 200 ng of Control/YAP1-S127A construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega ...
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bioRxiv - Genetics 2020Quote: ... and 100ng of Renilla luciferase control plasmid (Promega; Accession # AF025848) was mixed with 3μL of BioT transfection reagent (Bioland Scientific LLC ...
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bioRxiv - Cell Biology 2020Quote: Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] (cat# E8491, Promega) was used as template DNA in a PCR for obtaining the minimal promoter Luc2P together with the NF-κB response elements (NF-κB-RE) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with psiCHECK-2 plasmids (Promega, Madison, USA) containing two luciferase genes ...
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bioRxiv - Biochemistry 2023Quote: ... After overnight attachment the dual reporter plasmids (pGL4.36, pGL4.74 (Promega)) ...
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bioRxiv - Microbiology 2023Quote: ... and isolated with PureYield Plasmid Miniprep System (Promega, Cat# A1222). Sequences of all plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and the phRL-TK Renilla luciferase internal control plasmid (Promega). Reporter activity was evaluated 48 hours after transfection using a dual-luciferase assay according to the manufacturer’s protocol (Promega) ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was cloned into pGEM-T plasmid (Promega) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of Renilla luciferase expression plasmid (pRL-TK) (Promega) was co-transfected for transfection efficiency normalization (500 ng of DNA total was added per transfection) ...
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bioRxiv - Genetics 2023Quote: ... and the PureYield® Plasmid Midiprep Protocol System (Promega, USA). Verification of successful cloning was done by sequencing (Eurofins ...
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bioRxiv - Plant Biology 2023Quote: ... coli using PureYield™ Plasmid Miniprep (Promega, Madison, WI, USA), verified using Sanger sequencing (Laboratory of Biotechnology & Bioanalysis ...
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bioRxiv - Neuroscience 2023Quote: PCR reactions were ligated to pGEM T-Easy plasmid (Promega). Closed inserts were Sanger sequenced by the UChicago Comprehensive Cancer Center DNA Sequencing Facility ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of the IFN-β reporter plasmid (Promega, USA) and 4 ng of Renilla luciferase plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega) on an Amaxa Nucleofector 2b (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were done using FuGENE HD (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega) using lipofectamine 3000 ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Plant Biology 2020Quote: ... the PCR products were cloned into pGEM-T Easy plasmid (Promega) for propagation and sequencing (GATC Biotech ...
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bioRxiv - Synthetic Biology 2021Quote: All Plasmids were purified using the Midiprep kit (QIAGEN or Promega)
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bioRxiv - Cancer Biology 2021Quote: Plasmids were transiently transfected into 293T17 cells using FuGENE 6 (Promega). For these experiments pGL2M4-luc reporter plasmid37 (containing four CACGTG binding sites ...
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bioRxiv - Cancer Biology 2021Quote: ... The pSLIK-Hygro plasmid was transiently transfected by Fugene HD (Promega) in HEK293T cells together with the VSV-G ...
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bioRxiv - Genetics 2022Quote: ... and directionally cloned into the pGL3-Promoter plasmid (pGL3-P) (Promega) upstream of the SV40 promoter using restriction enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were cloned into a pGEM-T Easy plasmid (Promega, A1360). Ligation reactions were transformed into chemically competent cells for blue-white colony screening.
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bioRxiv - Molecular Biology 2020Quote: ... 100ng of the renilla luciferase expressing plasmid (pRL-SV40 Promega, E2231) was included as a transfection control ...
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bioRxiv - Biochemistry 2019Quote: ... Transfection of plasmids into cells were performed using FuGENE 6 (Promega) according to the manufacturer’s protocol and the cells were harvested 24 hours after transfection ...
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bioRxiv - Immunology 2019Quote: ... The NF-κB-Luc luciferase reporter plasmid was purchased from Promega.
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bioRxiv - Microbiology 2019Quote: ... 25 ng of a constitutively expressing Renilla luciferase plasmid (pRLTK, Promega) and the indicated viral protein expression plasmids – HEK293T cells ...
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bioRxiv - Genetics 2019Quote: ... plus pQCXIN-BirA plasmids using Fugene HD (Promega, Madison, WI, USA). Virus-containing medium was collected ...
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bioRxiv - Genetics 2020Quote: ... Each well was co-transfected with the pRL-TK plasmid (Promega) expressing the renilla luciferase as control ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or pB.2-eIF2α-S51A plasmids by use of FuGENE6 (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids were transfected into HEK293T cells using FuGENE HD (Promega, E2312), and proteins were allowed to express for 20 h ...
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bioRxiv - Cancer Biology 2021Quote: ... expression plasmids were delivered with Lipofectamine 3000 or FuGene 6 (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were transfected into HEK293T cells using FuGENE HD (Promega, E2312) and proteins were allowed to express for 20 h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Plasmids were purified using PureYield miniprep kits (Promega, Madison, WI, USA), and coding sequences were verified by complete sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... a total 3μg of plasmid DNA and 6μL FuGENE 6 (Promega) were combined in 300μL Opti-MEM (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid expressing the Halo tag (pFN21A) was purchased from Promega. The expression plasmids His12_PreScission _C1_C2B_MUN_C2C_tev_Halo and His12_PreScission _C1_C2B_MUN_tev_Halo (ΔC2C Munc13 ...
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bioRxiv - Microbiology 2021Quote: ... All cells were transfected with the renilla control plasmid siCheck (Promega). 100 ng of plasmid were transfected with LipoD293 according to manufacture’s protocol ...
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bioRxiv - Microbiology 2021Quote: N-terminal HaloTag KIF4 expressing plasmid (pFN21ASDB3041) was purchased from Promega. The N-terminal Myc-tagged KIF4 (both wild type and ATPase-null motor inactive mutant ...
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bioRxiv - Microbiology 2021Quote: ... The resulting fragment was inserted into the pGEM-T plasmid (Promega). A XbaI site was created by site directed mutagenesis with the primers 17176XbaF and 17176XbaR into the svfA coding sequence and a uidA-kanR cassette was inserted into this XbaI site ...
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bioRxiv - Microbiology 2020Quote: ... The control plasmid pRL-TK was obtained from Promega (Madison, USA). A pT7 Fluc containing plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... The pRL-SV40 plasmid was obtained from Promega (Madison, WI, USA). Plasmids for Ca2+ imaging such as pCMV G-CEPIA1er ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.4 ng plasmid encoding Renilla luciferase using FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection using the Dual Luciferase assay kit (Promega ...