Labshake search
Citations for Promega :
551 - 600 of 4762 citations for 7 bromo 1 methyl 1 3 dihydro 2H benzo d imidazol 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
-
bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
-
Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
-
bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
-
bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
-
bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
-
bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
-
bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
-
bioRxiv - Cell Biology 2022Quote: ... The gel pieces were rehydrated in trypsin solution (50 mM ABC pH 8.0, 1 or 2 µg trypsin per sample for proteome or BioID respectively, Promega) overnight at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
-
bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... D-luciferin (E1605, Promega) was added and luminescence signals were measured in triplicate using the Glomax® 96 Microplate Luminometer (Promega).
-
bioRxiv - Developmental Biology 2021Quote: ... anti-β-Galactosidase (1:1.000; Promega, 1:1000; abcam), anti-Cleaved Caspase-3 (1:500 ...
-
bioRxiv - Bioengineering 2020Quote: ... 1 µl random primers (50 ng·µl−1, Promega, C1181), 2 µL 0.1M DTT ...
-
bioRxiv - Biochemistry 2024Quote: ... 7C (mouse, Promega cat# N700A; 1:500-1:1000); Nluc in Fig ...
-
bioRxiv - Cell Biology 2021Quote: ... with that of the Halo 7-tag protein (Promega), with the insertion of a 63-base linker (21 amino acids ...
-
bioRxiv - Cell Biology 2022Quote: ... COS-7 cells were transiently transfected with FuGENE6 (Promega) with different StrepII- GFP-CSPP1 constructs for 24 hours ...
-
bioRxiv - Microbiology 2020Quote: ... followed by a reverse transcription using a universal degenerated oligo specific to all 5′ non-coding sequences of BTV-1 segments (BTV/Uni1; 5′ GTTAAAWHDB 3′) and the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA). The cDNAs obtained for the segment 7 were then quantified with a real time quantitative PCR (qPCR) ...
-
bioRxiv - Systems Biology 2023Quote: ... at the ratio of 1:50 for 3 h at 37°C and then using trypsin (sequencing grade modified trypsin; Promega, Fitchburg, WI, USA) at the ratio of 1:50 at 37°C overnight (for 15 h to 18 h ...
-
bioRxiv - Molecular Biology 2020Quote: ... one for use with the NADP+/NADPH Glo Assay and one for use in the NADH/NAD+ Glo Assay (Promega) such that all 4 metabolites will be measured on the same plate for each set of samples ...
-
bioRxiv - Genetics 2021Quote: ... 2-3 μl of the clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB ...
-
bioRxiv - Genomics 2021Quote: ... Variant or variant haplotype fragments were amplified in African American heterozygous individuals carrying the variants of interest located in the middle of the fragments (Supplementary Table 2 and 3) and cloned into the enhancer reporter vector pGL4.24 (E8421, Promega). Subsequently ...
-
bioRxiv - Genomics 2022Quote: Cell viability upon siRNA transfection was measured through the CellTiter 96® Non-Radioactive Cell Proliferation Assay (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT)) (Promega). 1× 104 microglia cells were plated per well in a 96 well plate and transfected with either ON-TARGETplus Non-targeting Control siRNA (Horizon Discovery) ...
-
Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: 2′3′-cGAMP (0.2 μM) and dsDNA (2 μg/mL) was transfected into cells using FuGENE HD Transfection Reagent (Promega), whereas the transfection reagent without dsDNA or 2′3′-cGAMP was added as the control ...
-
bioRxiv - Neuroscience 2023Quote: ... for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega, Cat# V5280), at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: ... Cell viability was assessed after 2 and 5 days (i.e., 3 and 6 days post-treatment) using CellTiter-Glo (Promega) according to the manufacturer’s guidelines ...
-
bioRxiv - Molecular Biology 2024Quote: ... In vitro transcribed and translated FLAG-tagged ERRα protein was made using the TnT reticulocyte reaction (Promega cat #L1170, 2h on 37°C) or the TnT wheat germ extract (cat #L5030 ...
-
bioRxiv - Microbiology 2022Quote: ... 40 µL of ONE-Glo reagent (Promega #E6110) added and luminescence measured using a Tecan Spark plate reader.
-
bioRxiv - Microbiology 2022Quote: ... 40 µL of ONE-Glo reagent (Promega #E6110) added and luminescence measured using a Tecan Spark plate reader ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 μl ONE-Glo™ luciferase reagent (Promega) was added to each well for 3-min incubation at 37°C ...
-
bioRxiv - Biophysics 2021Quote: ... 100 μl ONE-Glo™ luciferase reagent (Promega) was added to each well for 3-min incubation at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... using ONE-Glo™ Luciferase Assay System (Promega), according to the manufacturer’s specifications.
-
bioRxiv - Cell Biology 2021Quote: ... ONE-Glo™ Luciferase Assay System (Promega, E6110), Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2020Quote: ... One μg of Sequencing Modified Trypsin (Promega, V5117) was added and the samples were incubated at 37 °C for 16 hours under constant shaking (500 rpm) ...
-
bioRxiv - Immunology 2022Quote: ... equal volume of One-Glo reagent (Promega; E6120) was added to culture medium for readout using BMG PHERastar-FS plate reader ...
-
bioRxiv - Microbiology 2022Quote: ... 100 μL One-Glo luciferase substrate solution (Promega) was added to each well prior to reading the signal on the Envision plate reader (Perkin Elmer).
-
bioRxiv - Immunology 2022Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min ...
-
bioRxiv - Bioengineering 2022Quote: ... before reconstituted luciferase reagent (ONE-Glo EX, Promega) was added to cells in culture medium ...
-
bioRxiv - Microbiology 2021Quote: ... 60 μL of One-Glo-EX substrate (Promega) were added to each well and incubated on a plate shaker in the dark ...
-
bioRxiv - Immunology 2021Quote: ... 40 µL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 μL of ONE-Glo luciferase substrate (Promega) was added to each well ...
-
bioRxiv - Immunology 2021Quote: ... 40 µL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... One hundred ng SRE-Luc reporter (E134A, Promega), 300 ng Gαi or Gαi QL in pcDNA3.1+ ...
-
bioRxiv - Genomics 2023Quote: The RNase ONE Ribonuclease (catalog num. M4261, Promega) was used at this point to eliminate the Biotin groups at the 3’ end by degrading only the single-strand RNA present into the mixture ...
-
bioRxiv - Molecular Biology 2022Quote: ... One microliter of DNase I (Promega, Madison, WI), 2 microliters of RNasin Plus were added to each sample and samples were incubated at 37°C for 30 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to each well and incubated on a plate shaker in the dark for 5 min before reading the relative luciferase units using a BioTek Neo2 plate reader ...
-
bioRxiv - Microbiology 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to the cells and incubated for at least 5 minutes on a plate shaker in the dark ...