Labshake search
Citations for Promega :
501 - 550 of 4762 citations for 7 bromo 1 methyl 1 3 dihydro 2H benzo d imidazol 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were treated with the CellTiter-Glo 2.0 Viability Assay (1:2 v/v; Promega, Madison, WI) using the Viaflo Assist Pipetting Platform (Integra Biosciences ...
-
bioRxiv - Plant Biology 2024Quote: ... The transfected protoplasts were aliquoted into a 96-well plate with 200 nM D-luciferin/well with 3 biological replicates and then measured with a luminometer (Promega Max NAVIGATOR) every hour for 16 h.
-
bioRxiv - Genetics 2024Quote: ... Samples were diluted 1:1 with water and again 1:1 with 2X GoTaq Master Mix (Promega M712B) containing appropriate primers and amplified in 23 PCR cycles ...
-
bioRxiv - Cancer Biology 2020Quote: ... d-Luciferin (Promega), anesthetized with 2–3% isoflurane ...
-
bioRxiv - Neuroscience 2024Quote: ... For experiments cells were plated onto poly-D-lysine coated glass coverslips supplemented with 1 μM all-trans retinal and transfected using FuGENE® HD (Promega, Madison, USA) the next day ...
-
bioRxiv - Developmental Biology 2024Quote: ... 100 μM carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK) (Promega, Madison, WI, USA)) ...
-
bioRxiv - Immunology 2021Quote: ... 2.5 μl TDE1 and 22 μl nuclease-free water were combined and placed at 37°C for 3 min before 0.5 μl of 1% Digitonin (Promega, Cat. #G9441) was added to the master mix ...
-
bioRxiv - Zoology 2021Quote: ... cDNA (DFV, NDV, AIV, DHV-1, DHV-3) were prepared with viral RNAs using M-MLV Reverse Transcriptase (Promega, Wisconsin, USA). Bacterial genomic DNA (R ...
-
bioRxiv - Microbiology 2020Quote: ... This was followed by a second round of TBST washes before incubation with HRP conjugated goat anti-mouse Ab (1:5000 Ab in 3 % BSA in TBST; Promega Corp.) for an hour ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein was digested with Lys-C (Wako) (1:50 enzyme-to-substrate ratio) for 3 h at 25°C and with sequencing-grade modified trypsin (Promega, V5117) at 25°C for 14 h ...
-
bioRxiv - Immunology 2022Quote: ... Samples were diluted two-fold with 100 mM of triethylammonium bicarbonate (TEAB) and proteins were digested during 3 hours with 1 µg of trypsin (Promega V5111) and 1 µg of LysC (Wako 129-02541 ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times in TBST for 15’ per wash and incubated 1 hour in HRP-conjugated anti-rabbit (Promega, W4011) or anti-mouse (Promega ...
-
bioRxiv - Immunology 2024Quote: HEK293T cells (∼6 × 105 cells per 6-well) were transfected with 1-3 μg of the indicated constructs using FuGENE® HD Transfection Reagent (#E2311, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Supernatant was concentrated using PEG precipitation with 1 volume of PEG concentrator for every 3 volumes of supernatant (PEG concentrator: 40% (w/v) PEG 8000 (Promega, V3011) in 1× PBS (Thermo ...
-
bioRxiv - Microbiology 2023Quote: ... beads were suspended in digestion buffer (Tris 50 mM pH 7.5, urea 2 M, 1 mM DTT and 5 µg.µl of trypsin (Promega)) for 3 min at 30°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were washed twice with 50 mM HEPES, pH 8.5 (18,000g, 2 x 10min) before they were incubated with 1 μg trypsin (Promega) in 50 mM HEPES ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates at 3,000-5,000/well in serial 1:2 dilutions for 72 hours and assessed by CellTiter-Glo (Promega). For synergy assays ...
-
bioRxiv - Molecular Biology 2024Quote: ... and incubated with a secondary antibody (Gpx4 1:10000 or 4HNE 1:10000, W4011, Promega and Gapdh 1:10000 or ActB 1:5000, W4021, Promega), diluted in 10% TBS blocking buffer (Licor ...
-
bioRxiv - Immunology 2021Quote: ... A 1:1 dilution of Steady Glo (Promega) and Lysis Buffer were added to the cells and incubated at RT for 15 mins ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
-
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... 1 mM CaCl2 with 1 µg trypsin (Promega). Digested proteins were acidified with TFA to pH < 2 and RapiGest SF was precipitated out ...
-
bioRxiv - Neuroscience 2023Quote: ... luminescence photons were collected by accumulating the image for 60 s in the presence of 2 mM D-luciferin (Promega), and the luminescence signal was measured from the same varicosities as the corresponding fluorescence image ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 μL One-Glo-EX (Promega, E6120) was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer ...
-
bioRxiv - Microbiology 2021Quote: ... and 30U of RNase One (Promega, USA) in 1x DNase buffer (Ambion ...
-
bioRxiv - Microbiology 2021Quote: ... 30 μl of ONE-Glo reagent (Promega) was added according to manufacturer’s protocol and luminescence was quantified using the BioTek Synergy 2 Plate reader ...
-
bioRxiv - Plant Biology 2021Quote: ... A One-step RT-qPCR kit (Promega) was used for converting the RNA into cDNA before amplifying target transcripts on a CFX Connect Real-Time PCR Detection System (Biorad) ...
-
bioRxiv - Microbiology 2021Quote: ... or the QuantiFluor ONE dsDNA System (Promega). Indexed amplicons were pooled at equimolar concentration ...
-
bioRxiv - Neuroscience 2021Quote: ... The CytoTox-ONE Membrane Integrity Assay (Promega) was performed according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... One microgram of sequencing grade trypsin (Promega) dissolved in water was added to the protein solution and the specific proteolytic digestion proceeded for 16 h at 37 °C in a thermomixer (Thermo Fisher Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30 μL ONE-Glo reagent (Promega, E6120) was added to each well ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 40 units of RNase ONE (Promega) for 1.5 hr at 4°C with end-over-end rotation ...
-
bioRxiv - Microbiology 2020Quote: ... One μg of sequencing grade trypsin (Promega) was then added per sample and digestion was carried out overnight at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... and 100 µl One-Glo-EX (Promega) was added to the cells in equivalent MEM with no serum volume and incubated in the dark for 5 min ...
-
bioRxiv - Physiology 2022Quote: CellTiter 96 AQueous One Solution Reagent (Promega) was used to assess the effect of EVs on cell proliferation rate in in vitro autotransplantation approach (n=4) ...
-
bioRxiv - Microbiology 2023Quote: ... One μg of sequencing grade trypsin (Promega) was then added per sample and digestion was carried out overnight at 37°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... CellTiter 96 Aqueous One Solution Assay (Promega) was added (20 μL per well) ...
-
bioRxiv - Genetics 2023Quote: CellTiter 96 AQueous One Solution (Promega G3580) was added to fresh BM-MSC complete growth media ...
-
bioRxiv - Cancer Biology 2023Quote: ... CellTitre 96 AQueous One Solution MTS (Promega) was added to cells plated at low density according to manufacturer’s instructions and incubated in darkness in a humidified incubator for 3h at 37°C ...
-
bioRxiv - Microbiology 2023Quote: CellTiter 96 AQueous One Solution (G3580, Promega) was used ...
-
bioRxiv - Immunology 2024Quote: ... equal volume of One-Glo reagent (Promega) was added to the culture medium for readout ...
-
bioRxiv - Biochemistry 2024Quote: ... One microgram of sequencing grade trypsin (Promega), diluted in 50 mM ammonium bicarbonate ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA:FuGENE complexes were formed at a ratio of 1:3 (μg DNA/μL FuGENE HD) according to the manufacturer’s protocol (Promega, Madison, WI, USA). The resulting transfection complex (1 part ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with 1 µg of DNA diluted in 45 µl dMEM and then mixed with 3 µl ViaFect™ (Promega, USA) transfection reagent added and incubate for 20 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... approximately 200,000 HEK-293T cells were transfected with 1 μg of plasmid DNA complexed to 3 μL of FuGENE HD (Promega, Cat: E2311). 16-20 hours post transfection the cells were dissociated from the plastic using cell dissociation buffer (Gibco Cat ...
-
bioRxiv - Molecular Biology 2020Quote: Cell viability was assayed with an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) colorimetric assay (Promega) per manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... cells were treated with 3 µM PP242 for 2 h and lysed with Passive Lysis Buffer (Promega, E194A). Luminescence was detected with a Dual- Luciferase Reporter Assay System (Promega ...
-
bioRxiv - Biophysics 2024Quote: ... UCSD) was transiently transfected into RPE1 cells to labeling peroxisomes for 2 or 3 days using Viafect (Promega) according to the manufacturer’s recommendations ...
-
bioRxiv - Genomics 2022Quote: ... Quantification of DNA was measured using fluorescence intensity of Quantifluor One dsDNA dye (Promega Quantifluor One dsDNA dye; E4871), inside a 0.5 milliliter thin-walled PCR tube ...