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Citations for Promega :
501 - 550 of 1703 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... They were then digested with mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Pathology 2023Quote: ... MS-grade trypsin and Lys-C were from Promega (Madison, WI). All other chemicals and reagents were pu0072chased from Sigma-Aldrich (St ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM DTT containing 10 ng/μL trypsin/Lys-C mixture (Promega), and incubated overnight at 25 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin/Lys-C mix (V5071, Promega, Madison, WI). The resulting peptides were cleaned up with Acclaim™ PepMap™ 100 C18 spin columns (SEM SS18V ...
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bioRxiv - Plant Biology 2023Quote: ... Lys-C digest was further digested with 0.1 μg trypsin (Promega) with shaking at 1,000 rpm at 37°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then proteolyzed with Trypsin/Lys-C Mix (Promega, V5071) at a 25:1 protein ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested overnight at 25°C with modified trypsin (Promega) using an enzyme-to-substrate ratio of 1:50 in 100 mM ammonium bicarbonate ...
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bioRxiv - Physiology 2024Quote: ... Overnight digestion with Lys-C/Trypsin combo (1:50 enzyme:protein; Promega). After stopping the digestion with 1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2024Quote: ... stored at −80°C and analysed using Dual-luciferase substrate (Promega) in a FLUOstar Optima luminometer (BMG labTech) ...
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bioRxiv - Plant Biology 2024Quote: ... Lys-C digest was further digested with 0.1 μg trypsin (Promega) with shaking at 1,000 rpm at 37°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were incubated with a Trypsin/Lys-C mix (Promega #V5071) for 1 hr at 47 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested overnight with a trypsin/Lys-C mix (Promega) at a 1:50 protein ratio in 100 mM ammonium bicarbonate (NH4HCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega V5113). Then ...
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bioRxiv - Developmental Biology 2024Quote: ... 30 sec extension at 72°C using GoTaq polymerase (Promega # M7122). A final extension of 72°C completed the PCR reactions ...
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bioRxiv - Cell Biology 2020Quote: ... 1,3 μg of plasmid DNA and 3 μl of FuGene 6 (Promega) were mixed in 100 µl Opti-MEMTM medium before the addition to the dish ...
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bioRxiv - Developmental Biology 2020Quote: ... which was co-transfected with 3 ng phRG-TK Renilla vector (Promega) as normalization control ...
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bioRxiv - Molecular Biology 2021Quote: ... transfected with 1 µg DNA and 3 µl FuGENE HD (Promega, E2311) in 150 µl Opti-MEM media according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5µg was analyzed using the Caspase 3/7 glo kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and caspase activity was measured by Caspase-Glo 3/7(Promega, G8092).
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bioRxiv - Immunology 2021Quote: ... after added 100 mL of Caspase-Glo 3/7 reagent (Promega, USA), the plate was gently shaken at room temperature for 2 h and the luminescence was measured by a plate-reading luminometer.
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured using the Caspase-Glo® 3/7 Assay (Promega) based on the manufacturer’s instructions and our previous work (21 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cellular apoptosis was analyzed with Caspase-Glo 3/7 assay kits (Promega), which measures Caspase-3/7 activity ...
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bioRxiv - Cell Biology 2020Quote: ... in 2× SSC supplemented with 3% (vol/vol) RNasin Ribonuclease inhibitor (Promega), 6% (vol/vol ...
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bioRxiv - Microbiology 2021Quote: ... and pVSV-G (4:3:1 ratio) using calcium phosphate transfection (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 ng of the Renilla Luciferase expressing pRL-SV40 plasmid (Promega) for normalization ...
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bioRxiv - Cell Biology 2021Quote: ... 3) TGFβ2 (2.5 ng/ml) + U0126 (10 μM; Promega, Madison, WI, USA), 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Apoptosis of cells was determined using Caspase-Glo 3/7 Assay (Promega) according to the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2022Quote: ... and anti-activated Caspase 3 (rabbit, Promega, catalog #G7481, 1:250, RRID:AB_430875), and appropriate secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... amplicons were ligated into the Renilla luciferase 3’UTR of psiCheck2 (Promega) vector linearized with Xho-I and Not-I ...
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bioRxiv - Neuroscience 2021Quote: ... 1.0 μM 431R2 [5’-CTCTTCACAACAGTCATGTGCG-3’] and 1.0 U GoTaq2 polymerase (Promega). Cycling conditions were 30 s at 98°C ...
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bioRxiv - Microbiology 2020Quote: Caspase 3/7 activity was measured by using Caspase Glo reagent (Promega). MDA-MB-231 and MDA-MB-436 cells were adsorbed with reovirus at an MOI of 500 PFU/cell for 1 h at room temperature or treated with 50 μM etoposide ...
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bioRxiv - Plant Biology 2022Quote: ... followed by 3 μL sequencing-grade trypsin solution (1 μg/μL, Promega), and the sample was incubated for 12 h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Promega Caspase-Glo™ 3/7 Assay Kit was obtained from Promega Corporation (Madison ...
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bioRxiv - Molecular Biology 2023Quote: ... by addition of 3 μl Renilla-Glo™ reagent (Promega, Cat# E2710) and measuring the luminescence signal with the ViewLux uHTS Microplate Imager (PerkinElmer ...
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bioRxiv - Neuroscience 2024Quote: ... and the Caspase-Glo 3/7 assay kit (#G8090, Promega, Nacka, Sweden) were used in 96-well plates (Sarstedt #82.1581.001 ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μL of 1:100 diluted NanoBRET Nano-Glo substrate (Promega N1571) was added ...
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bioRxiv - Cell Biology 2023Quote: ... Caspase-Glo 3/7 Assay Systems (cat. #8090, Promega, Madison, WI, USA); AlexaFluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... plate lids were removed and 3 μL of CellTiter-Glo One (Promega) were added to each well by using a solenoid valve dispenser ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μL of BrightGlo reagent solution (Promega, diluted 1:3 in water) was added to each well after 24 h of compound treatment ...
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bioRxiv - Physiology 2023Quote: ... and the Caspase-Glo 3/7 Assay Kit was purchased from Promega.
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bioRxiv - Immunology 2023Quote: ... and were lysed 3 days post-infection using 1x lysis buffer (Promega) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa-HA cells were transfected with 3 μL of FuGENE 6 (Promega) and 1 μg pA2 plasmid DNA per well of a six well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa-HA cells were transfected using 3 μL of FuGENE 6 (Promega) and 0.5 μg Alu reporter plasmid + 0.5 μg TMO2F3 plasmid DNA per well of a six well plate as noted above ...
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bioRxiv - Pathology 2020Quote: ... the 60°C annealing/extension temperature recommended by the mastermix manufacturer (Promega) produced more robust amplification (data not shown).
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bioRxiv - Biochemistry 2021Quote: ... and finally digested overnight at 37 °C with 375 ng Trypsin (Promega). The peptides were extracted from the gel twice using 100 μl of 50 % acetonitrile and 0.25 % TFA buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 30 s at 68°C or through GO taq polymerase (Promega) for 2 min at 95°C followed by 25 cycles of 30 s at 95°C ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteolytic digestion was carried out with a trypsin/Lys-C mixture (Promega) incubated for 18 h at 37°C at a protein to protease ratio of 25:1 ...
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bioRxiv - Cell Biology 2021Quote: ... 60°C for 1 minute) using Go-Taq qPCR Master Mix (Promega) on a QuantStudio™ 7 Flex Real Time PCR System (ThermoFisher Scientific) ...