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Citations for Promega :
451 - 500 of 1703 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C with trypsin (Promega) with an enzyme ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl2 and 500 ng Trypsin Lys-C (Promega). On bead digest was carried out overnight at 37°C while shaking at 200 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested 16 hours at 37°C with trypsin (Promega) and stopped by addition formic acid (FA ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested by Trypsin/Lys-C Mix (Promega). After the digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... firstly with Endoproteinase Lys-C (ratio 1:33 enzyme:lysate) (Promega) for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate ...
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bioRxiv - Neuroscience 2023Quote: ... In-gel digestion with Trypsin/Lys-C Mix solution (Promega) was performed as previously described (Gonzalez-Lozano & Koopmans ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of 0.1 µg/µL Trypsin/rLys-C (Promega) in 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Biochemistry 2024Quote: ... and Glu-C (V1651) were obtained from Promega (Finnboda, Sweden).
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bioRxiv - Cell Biology 2023Quote: ... Sequencing-grade porcine trypsin/Lys-C mix (Promega, Madison, WI) was added ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequencing-grade endopeptidase Trypsin/Lys-C Mix (Promega) was added at an enzyme–substrate ratio of 1:50 wt/wt ...
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bioRxiv - Physiology 2023Quote: ... and digested (0.4 ug of Lys-C/Trypsin mix, Promega). After 4 hours of digestion in the urea buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Cell Biology 2024Quote: ... containing 2 µg of Trypsin/Lys-C mix (Promega V5071) was added to each S-trap column ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 15°C in the presence of 27% PEG 8000 (Promega) to minimize ligation sequence bias ...
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bioRxiv - Cancer Biology 2024Quote: ... proteolytic digestion was performed using Trypsin/Lys-C Mix (Promega) with the enzyme-to-protein ratio of 1:25 (m/m) ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... of selected 3’UTRs into the pmirGLO vector (Promega, Southampton, UK) was used to generate 3’UTR luciferase reporters essentially as previously described (9) ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was transfected with 3 uL FuGENE 6 reagent (Promega) and 130 ng pcDNA3-mouseSTING in line with manufacturer’s protocols.
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bioRxiv - Microbiology 2021Quote: ... 3 μg of total RNA was treated with RQ1 DNase (Promega), and then purified by phenol chloroform extraction and ethanol precipitation ...
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bioRxiv - Cancer Biology 2020Quote: ... Apoptosis was determined using Caspase 3/7-Glo assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly add 3 µL 1:1 water diluted digitonin (Promega G9441)) was added ...
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bioRxiv - Immunology 2021Quote: ... then each well was diluted 1:3 with TE buffer (Promega). A 2.7 μL aliquot from each sample was mixed with 2.5 μL of SsoFast EvaGreen Supermix with Low Rox (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... or 250 ng DNA (Nup54-mEGFP) and 3 µL FuGENE6 (Promega) in Opti-Mem I (Gibco ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples from liquid culture were placed on 3% agarose pads (Promega) containing M9 minimal media and sandwiched between glass coverslips to immobilize the cells for imaging ...
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bioRxiv - Biochemistry 2024Quote: ... using random primers and oligo-dT primers (3:1 mol) (Promega). The obtained cDNA was stored at −20 °C until further use.
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bioRxiv - Microbiology 2023Quote: ... and a Victor 3 or GloMax Navigator luminometer (Perkin Elmer/Promega). The 50% and 80% inhibitory concentrations (IC50 and IC80 ...
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bioRxiv - Immunology 2023Quote: ... (3) We used the ProNex Size-Selection DNA Purification System (Promega) to purify PCR products ...
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bioRxiv - Cancer Biology 2023Quote: ... we used Caspase-Glo 3/7 and 8 assay systems (Promega). Approximately 2.0 × 103 cells/well were seeded in a 96-well plate and (1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µl/well of room temperature Caspase Glo 3/7 (Promega) reagent was added to the treated and control cells ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg plasmid was combined with 3 µl FuGENE (Promega, E2311) in OptiMEM medium for each ml of culture medium ...
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bioRxiv - Cancer Biology 2024Quote: Caspase activity was measured using Caspase-Glo® 3/7 (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis was measured using the Caspase-Glo 3/7 Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1h at 37°C in presence of random hexamers (Promega). qPCR primers (supplement Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μl of Trypsin/Lys-C mix (0.5μg/μl, Promega, V5073) was added to each sample and incubated for 3 hrs at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were purified using C18 spin columns (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega) using S-Trap (Protifi ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μg/mL Mass Spec Trypsin/Lys-C Mix (V5073, Promega) and 1 mM DTT was added to the samples ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
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bioRxiv - Cell Biology 2021Quote: ... digested overnight with Trypsin/Lys-C (1:20 w/w, Promega), desalted using C18 Hypersep Spin Column (Thermo) ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were cleaned up using C18 spin columns (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7 and digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Immunology 2022Quote: ... dehydrated and proteolytically cleaved overnight at 37 °C with trypsin (Promega). The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... The LgBiT fragment amplified from pBiT1.1-C [TK/LgBiT] Vector (Promega) using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µg Lys-C/Trypsin mix protease (Promega, Mass spec grade) were added to each sample (10 mM EPPS pH 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... in-gel tryptic digestion (250 ng Trypsin-Lys-C mix Promega V5072 in 50 mM ammonium bicarbonate overnight at 37oC) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37°C with 1µg trypsin (Promega). Peptides were desalted on C18 StageTips ...