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Citations for Promega :
501 - 550 of 4976 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were directly sequenced using PCR primers after purification with Gel Clean-up System (Promega Corporation, Madison, WI, USA). All sequencing reactions were done on ABI 3700 DNA automated sequencer with the BigDye chemistry (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Real time quantitative PCR was performed in a CFX 96 BioRad CFX 96 Real-Time PCR detection instrument with GoTaq qRT-PCR master mix (Promega, A6002). The sequence of the primers used in this study are ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR amplicon from the second-round PCR was gel-purified and ligated to the pGEMT- Easy vector (Promega, USA), as per the manufacturer-recommended protocol (Promega ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Quantitative PCR (qPCR) was then performed on LightCycler 480 Real-Time PCR System using the GoTaq qPCR Master Mix (Promega, #A6001), and the primers pairs are listed in Table S7 ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Microbiology 2023Quote: ... The three PCR fragments were ligated by PCR using primers P1/P3 and cloned into pGEM®-T easy vector (Promega). Plasmid was then digested with NotI ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction with a total volume of 25 μL contained 12.5 μL of PCR master mix (Promega; Madison, WI, USA), 8.0 μL of nuclease free water ...
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bioRxiv - Plant Biology 2024Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Genomics 2024Quote: ... A total of 10 µL at a minimum concentration of approximately 100 ng/µL of each PCR amplicon was used to express TF proteins in vitro using the TnT T7 Quick for PCR DNA System (Promega L5540) with an incubation at 30⁰C for 2 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-PCRs were conducted in two-step reactions by using two-step RT-PCR: M-MLV reverse transcriptase in M-MLV RT-PCR system (Promega, Madison, WI) and Platinum Taq polymerase (GeneMark ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry and CLIP were PCR amplified from pFN21A (Promega), pSNAPf (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... for RT-PCR and GoTaq qPCR Master Mix (Promega) for RT-qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Genotypes were determined by PCR with GoTaq Green (Promega) or allelic discrimination for MommeD1 or MommeD43 ...
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bioRxiv - Cell Biology 2022Quote: ... Wizard SV Gel and PCR Clean-Up System (ProMega) was used on cDNA samples ...
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bioRxiv - Developmental Biology 2022Quote: ... RT (reverse transcription) PCR was performed using GoTaq (Promega). For quantitative RT-PCR analysis ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR was done with the GoTaq Polymerase (Promega) using forward primer (5’-ACGTGGAGCCCAACTTTGTA-3’ ...
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bioRxiv - Genetics 2020Quote: ... and 200 ng PCR product using FuGENE HD (Promega). At 2 days post transfection ...
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bioRxiv - Cancer Biology 2021Quote: Transcription templates were synthesized by PCR (GOTAQ G2, Promega) using a hybrid T7promoter/sequence-specific forward primer and a sequence-specific reverse primer ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were digested with SacI and KpnI (Promega) and ligated in the pZsGreen1-1 plasmid ...
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bioRxiv - Microbiology 2020Quote: PCRs were performed in 25 μl reaction buffer (Promega) with MgCl2 at 1.5mM final ...
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bioRxiv - Neuroscience 2020Quote: ... NanoLuc was PCR amplified from plasmid pNL.1.1 (Promega) and cloned into pUS2-MT to generate pUS2-MT-Nanoluc ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using GoTaq G2 DNA Polymerase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... were amplified by PCR from human genomic DNA (Promega), using the primers listed in Table S1 and then inserted into the psiCHECK-2 vector ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR products were cloned into pGEMT (Promega #1360) and sequenced to verify the mutations ...
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bioRxiv - Microbiology 2020Quote: ... sucrose-resistant colonies by PCR using GoTaq® (Promega) and primers listed in Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was subcloned into plasmid psiCHECK2 (Promega) previously digested with NotI using the In-Fusion cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using GoTaq Green master mix (Promega), per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were performed using Master Mix (2X) (Promega) and carried out at an initial denaturation step of 95 °C for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... PCR screens were carried out using GoTaq Green (Promega) with the primer combinations described.
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR product and pHis6-Halo (Promega Corporation) were digested with PvuI and NotI and ligated to create pVJS77 that was confirmed via sequencing (Georgia Genomics Facility ...
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bioRxiv - Biochemistry 2020Quote: PCR was performed using GoTaq Green Master Mix (Promega) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Genetics 2021Quote: ... the coding sequence was PCR amplified from pFN31K (Promega) using forward ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR reactions were performed using GoTaq DNA Polymerase (Promega) following manufacturer protocols ...
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bioRxiv - Genetics 2021Quote: ... PCR was performed with GoTaq DNA polymerase (Promega #M3005) using the following program ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus MMLV reverse transcriptase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned into vector pGEM-T (Promega). CRISPR A and B start naturally with a 6 nt cassette consisting of adenines and guanines that we used for synchronisation of transcription (see below) ...
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bioRxiv - Microbiology 2023Quote: ... unlabeled PCR was cloned into pGEM-T Easy (Promega) and positive clones were checked by amplification with the vector-based primers M13f and M13R.
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bioRxiv - Physiology 2023Quote: ... The PCR products were subcloned to T-vector (Promega) and sequenced ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping PCRs were performed using GoTaq Green Mastermix (Promega) (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... Genotyping PCRs were conducted using GoTaq DNA Polymerase (Promega) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2 ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... transferred to a PCR clean-up column (Promega, A9282) and centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The mutagenesis of the EPIYA sites were done one after the other using the kit Quick change II site-directed mutagenesis (Aligent #200523) ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The cagA fragment was digested with KpnI and EagI and subcloned into the lentivirus entry vector pEF-1α/pENTR (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... and a Go-Taq real-time PCR system (Promega). The data were analyzed by the ΔΔCT method and normalized to ACT1 in each sample ...