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Citations for Promega :
5301 - 5350 of 5375 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the cells were harvested in the lysis buffer and the fluorescence value was measured by a dual luciferase assay kit (Promega, Madison, WI, USA).
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified with the Nucleospin gel and PCR clean-up kit (Macherey Nagel) and transcribed in vitro using T7 RNA polymerase (RiboMAX system; Promega, Madison, WI, USA). RNA was purified from the sample using the Nucleospin RNA clean-up kit (Macherey Nagel) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Total RNA was extracted from the antennae (50 individuals) of soldiers and workers using an SV Total RNA extraction kit (Promega, Madison, WI, USA). The amount of RNA and DNA in each sample was quantified using a Qubit fluorometer (Life Technology ...
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bioRxiv - Synthetic Biology 2022Quote: ... The viability of Vero-E6-Luc2+ cells was assessed using either CellTiter-Glo™ Luminescent Cell Viability Assay Kit (Promega, Cat# PR-G7570) or One-Glo® assay (Promega ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA extraction was performed using a lysozyme/mutanolysin-based cell lysis followed by purification using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA). Library preparation on extracted DNA was conducted using a Kapa kit (Roche ...
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bioRxiv - Molecular Biology 2022Quote: These recombinant plasmids were co-transfected with the components of Dharmacon™ Trans-Lentiviral packaging kit into HEK293FT cells using FuGENE® HD (Promega Corp.) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Molecular Biology 2022Quote: ... the transfected cells plated in 96-well black plates were examined using a CellTiter-Blue Cell Viability Assay kit (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Systems Biology 2022Quote: ... Resistance against ribociclib was detected by the alteration of the dose–response curve measured using CellTiterGlo Chemiluminescent Kit (Promega Corporation, Cat. No.: G7573).
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bioRxiv - Cancer Biology 2024Quote: ... cell viability and cytotoxicity were measured with CellTiter-Glo Luminescent Cell Viability Assay with CellTox Green Cytotoxicity Assay (Promega kits G7570 and G8741).
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bioRxiv - Neuroscience 2024Quote: NAD and NADP levels were measured using the commercially available kits NAD/NADH-Glo Assay and NADP/NADPH-Glo Assay (Promega G9071, Promega G9081), respectively ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2023Quote: ... globosa gut isolates (KCTC 37188 and KCTC 37189) and skin isolates (KCTC 27541 and KCTC 27777) was extracted using the Wizard HMW DNA Extraction Kit (Promega, Madison, WI, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The relative light unit value of luciferase was determined using the PicaGene Luminescence Kit (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... DNA was purified from the samples using the Maxwell 16 Tissue LEV Total RNA purification Kit custom-adapted for DNA extraction (cat no. AS 1220, Promega, Madison, Wisconsin, USA) in a Maxwell 16 MDx instrument (Promega) ...
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bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using Quantus™ Fluorometer (Promega, Madison, WI, USA). The Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA was resuspended in 1× IDTE buffer (Integrated DNA Technologies, Coralville, IA, USA) and quantified using the QuantiFluor One dsDNA kit (Promega, Madison, WI, USA). The HiFi reads were obtained using the SMRT Cell 8M and the PacBio Sequel II instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910). Luminescence was measured using a microplate reader (TECAN infinite M200pro) ...
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bioRxiv - Cancer Biology 2023Quote: ... Authentication of cell lines was confirmed regularly by short tandem repeat (STR) profiling by using the GenePrint 10 kit (Promega, Madison, WI, USA) on an ABI 3730 DNA analyzer (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was isolated from frozen harvested materials using the SV Total RNA isolation kit with on-column DNA digestion (Promega, Madison, WI, USA). The purity of all RNA samples was confirmed using a NanoDrop2000 instrument (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... senegalensis DNA was extracted from muscle tissues in thoraxes using Maxwell® 16 LEV Plant DNA Kit (Cat. No. AS1420, Promega, WI, USA). Details on extraction and library preparation protocols are provided in the Supporting Text 1.
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bioRxiv - Cancer Biology 2023Quote: gDNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue sections was carried out using Maxwell 16 FFPE plus LEV DNA purification kit (AS1720; Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... coronary effluent was collected for detection and quantification of lactate dehydrogenase (LDH) using the Promega luminescent LDH-Glo 105TM Cytotoxicity Assay kit following manufacturer protocol (Promega, Madison, WI, USA).
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bioRxiv - Neuroscience 2023Quote: ... was reverse transcribed with a GoScriptTM Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation, an affiliate of Promega Biotecnologia do Brasil, Ltda). Primers were designed and synthesized by IDT-DNA (San Diego ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we digested tissues using Proteinase K enzyme and used a Maxwell® Rapid Sample Concentrator Instrument with Maxwell® 16 Tissue DNA Purification Kit (Promega Corporation). We used 34 cycles polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2024Quote: ... for intra-subcellular (i.e., vacuolar) conversion of glutamine (Gln) to glutamate (Glu) assay using Glutamine/Glutamate-Glo Assay kit (Promega, Madison, WI; Cat. # J8021) following kit’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase activities were conducted 24 hours after transfection in the cells treated with or without the TEAD inhibitor CP1 using the dual-luciferase reporter kit (cat. E1910, Promega, Madison, WI, USA). Assays were conducted in triplicates and quantified using PerkinElmer EnVision plate reader ...
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... BP1425-500) at 37 °C and the plasmid was isolated from single colonies using the PureYield Plasmid Midiprep kit (Promega, Cat no. A2495), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The amplified product was used as a template to synthesize dsRNA using a RiboMAX Large Scale System-T7 Kit (Promega, Madison, WI, USA). The dsRNA was then isolated and purified ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from cell lines was isolated using Maxwell® 16 Instrument and the Maxwell® 16 LEV simplyRNA Cells Kit (Promega Cat.# AS1270) using the protocol provided ...
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bioRxiv - Bioengineering 2022Quote: ... was performed to measure the specific killing activity of anti-HIV CAR-T cells toward LHL2/3 cells and LEL6 cells at different ratios from 1:1 to 10:1 by using the CytoTox 96 non-radioactive cytotoxicity kit (Promega, Madison, WI, United States) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Silica-dried samples were processed using a combination of bead beating (Retsch MM400, Haan, Germany) and the Maxwell® 16 DNA Purification Kit (Promega, Madison, WI, USA) and are described in detail in supplementary information ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Individual genotyping at every generation was performed via PCR on genomic DNA extracted from larvae pseudopods (Wizard® Genomic DNA Purification Kit, Promega, Madison, WI, USA) using gene-specific primers (forward ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA was extracted from the tumor tissue and blood samples previously frozen using Maxwell® RSC Tissue DNA Kit (cat# AS1610, Promega, Madison, WI, USA) and Maxwell® RSC Blood DNA Kit (cat# AS1400 ...
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bioRxiv - Physiology 2019Quote: Total RNA in the root tips (0-10 mm) was extracted using Eastep® Super Total RNA Extraction Kit (Shanghai Promega Biological Products Ltd). Gene-specific primers were designed by the National Center for Biotechnology Information (NCBI ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170, Promega Co., Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... was reverse transcribed with a GoScript™ Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation, an affiliate of Promega Biotecnologia do Brasil, Ltda). Primers were designed and synthesized by IDT-DNA (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... The resulting suspension was then centrifuged at 14,000 rpm at 4°C to remove cellular fragments and the genomic DNA finally was extracted using the Wizard® Genomic DNA Purification Kit reference A1125 (Promega, Madison, WI, USA) following the bulla guidelines ...
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bioRxiv - Microbiology 2022Quote: ... then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using a Quantus™ fluorometer (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2019Quote: ... genomic DNA were isolated from both the parental UA159 and each of the seven RSV3s using Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA) with some modifications ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from overnight bacteria cultures from resistant and wild type parental strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), quantified and measured for DNA quality by Qubit High Sensitive dsDNA assay (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... faginata MAT1-1 isolate using a CTAB-chloroform DNA extraction method (van Diepen et al. 2017) and a MAT1-2 isolate using a Wizard® kit (Promega, Madison, WI, USA) and suspended in 75 µl Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... AccuPrep® Plasmid Mini Extraction Kit (Bioneer Corporation, Republic of Korea) and Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, USA) were used for plasmid and PCR product extraction and purification ...
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bioRxiv - Pathology 2020Quote: Messenger RNA was isolated from cells or slices (three slices per sample, pooled, homogenized prior to extraction) using Maxwell® LEV Simply RNA Cells/Tissue kit (Promega, Madison, Wisconsin, US). A NanoDrop® ND-1000 Spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each sample taken from captured birds and carcasses using the Wizard Genomic DNA Purification kit (Promega Corp., Madison, Wisconsin, USA) according to the manufacturer’s instructions and eluted in 125 µl of DEPC-treated water ...
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bioRxiv - Plant Biology 2020Quote: Degenerate primers specific to each gene were designed using Primer3plus software [https://primer3plus.com/] for all 17 identified CKX genes following the guidelines for primer design provided by the PCR amplification kit manufacturer (Promega Corporation, USA; Table S2). PCR amplification of the identified CKX GFMs was performed using the reaction kits selected based on the gene size ...