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Citations for Promega :
4901 - 4950 of 5375 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality was assessed with an Agilent Bioanalyser and the quantity was calculated by an RNA-specific kit from Quantus (Promega).
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bioRxiv - Microbiology 2023Quote: High-molecular weight genomic DNA was isolated based on a modified version of Promega’s Genomic DNA Isolation Kit (Promega, A1120) [63] ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from frozen ground tissues using the innuPREP Plant RNA kit (Analytic Jena) and digested with RQ1 DNase (Promega). First-strand cDNA synthesis was conducted on 1 µg RNA using the SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and then collected and used to quantify lactate dehydrogenase (LDH) release (in a 50 μl aliquot) using the CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Cat No. G1780, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Wizard™ Plus SV Minipreps DNA Purification System and Wizard™ Genomic DNA Purification Kits were purchased from Promega, U.S.A ...
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bioRxiv - Genetics 2023Quote: ... Luciferase activity in cell lysates was assayed 48 hours post-transfection using the Dual Luciferase Reporter Assay kit (Promega E1910), and the Fluoroskan Ascent FL microplate reader (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: High molecular weight DNA was isolated from spleen tissue of a single male from each of the 11 imported Nachman wild-derived inbred strains using the Wizard DNA Purification Kit (Promega) or the Monarch HMW DNA kit (NEB ...
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bioRxiv - Microbiology 2023Quote: The intracellular ATP content of bacteria suspended in mineral water was determined using the luciferase-based BacTiter-Glo Microbial Cell Viability Assay kit (Promega). As per the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid carrying cells were cultured overnight in DM250 supplemented with 50 μg/ml kanamycin and DNA isolated using a Wizard Genomic DNA Purification Kit (Promega). Amplification of target genes was carried out using a SYBR Green based qPCR mix consisting of Q5® High-Fidelity 2× Master Mix (NEB) ...
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bioRxiv - Microbiology 2023Quote: The glycosyltransferase activity of the CslA-GlxA complex was quantitatively measured by using the UDP-GloTM glycosyltransferase assay kit (Promega). This assay detects the content of free UDP ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with 2% 1-thioglycerol using a Bio-Gen PRO200 homogenizer (PRO Scientific, Oxford, USA) and then extracted RNA using the Maxwell RSC simplyRNA Tissue Kit (Promega). We quantified extracts using a Quant-IT RNA Assay Kit ...
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bioRxiv - Cell Biology 2023Quote: NAD(P)+ and NAD(P)H concentrations were quantified with NAD(P)- and NAD(P)H-Glo assay kits (Promega) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated from peripheral blood samples using the Maxwell RSC Blood DNA kit (Promega, Cat# AS1400 using the Maxwell RSC benchtop automated DNA/RNA extraction instrument (Promega). Total CER-1236 T cells present in the blood was determined using ddPCR as previously described (17) ...
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bioRxiv - Cancer Biology 2023Quote: Caspase3/7 activity was quantified in cell lysates using the Kit Caspase-Glo® 3/7 Assay Systems (G8091, Promega).
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bioRxiv - Biochemistry 2023Quote: Cells were grown in opaque 96-well white plates and then processed according to the manufacturer’s instructions of the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega # G9241). The standard curve of ATP was determined in parallel for absolute quantitation.
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bioRxiv - Bioengineering 2023Quote: ... PCR products were confirmed on a 0.8% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up kit (Promega). DNA fragments were quantified using Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... PB-mClover3-H2B::PGK-BSD and PB-mRuby3-NPM1::PGK-Puro were co-transfected with CAG-PBase into HeLa S3 using a FUGENE HD transfection kit (Promega). Stable clones were obtained by sequential Puromycin and blasticidin selection ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf samples of the high dyad producing plants were stored at -80°C before DNA extraction with the Wizard genomic DNA purification kit (Promega). A pooled DNA sample (equal mass ...
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bioRxiv - Cell Biology 2023Quote: ... and used as template to generate dsRNA in vitro using the Promega T7 RibomaxTM Express RNAi System kit (Promega, P1320). The dsRNA was precipitated with sodium acetate (300 mM final ...
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bioRxiv - Microbiology 2023Quote: ... core and modified histones and transcription factors at the HIV-1 LTR was assessed by quantitative PCR using primers spanning the full promoter (Table 1) with GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and used the GoScript Reverse Transcription System kit to generate cDNA from extracted RNA (Catalog #A5000, Promega Corporation, Madison, WI). We designed primers using the tool primer-Blast134 and the predicted cDNA sequence of kitlga (Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: The levels of glutathione (GSH) of GECs were determined using a luminescence-based GSH/GSSG-Glo Assay kit (Promega, V6611) [41] ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated from the cell lysis using Maxwell® RSC simply RNA Cells/Tissue Kit (Promega, Madison, WI, USA) and cDNA was synthesized ...
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bioRxiv - Cancer Biology 2024Quote: ... Positive cells were selected using antibiotic Zeocin and Luciferase reporter assay was performed using Dual- Glo luciferase assay kits (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... HBEC cells were incubated for an additional 24 hours and then luciferase activity was measured using the Dual-Luciferase Reporter assay kit (Promega) on a Glomax instrument (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: ... The 4 µg of linearized vectors were transcribed 5h using RiboMAX™ Large Scale T7 RNA polymerase kit (Promega, #P1300) with 5.6 mM Ribo m7G Cap Analog (Promega ...
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bioRxiv - Developmental Biology 2024Quote: The efficacy of all MOs was verified in western blots following in vitro transcription and translation (TNT-coupled Wheat Germ Extract kit, Promega) of pCMV-Sport6-marcks.S ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...
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bioRxiv - Microbiology 2024Quote: SAM-dependant N6mA DNA methylation activity of PglX was probed in vitro using an MTase-Glo Methytransferase Assay kit (Promega). The kit allows indirect measurement of SAM-dependent methyltransferase activity via production of the SAH reaction product ...
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bioRxiv - Molecular Biology 2024Quote: ... Macroscopic dissection of tumor areas was performed with sterile scalpels and tissues were extracted using the Maxwell RSC FFPE Plus DNA Purification Kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... In a 96 well plate (white bottom) 20 µL lysate was mixed with 50 µL Luciferase Assay Reagent (#E1501 kit, Promega) (as per the manufacturer’s instructions) ...
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bioRxiv - Immunology 2024Quote: ... The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH) assay to assess cell death using a CytoTox 96 non-radioactive cytotoxicity assay kit (Promega). Both the supernatants and cell pellets were collected for immunoblotting analyses.
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bioRxiv - Immunology 2024Quote: Cell pellets from cells stimulated with inflammasome activators were used to measure cell viability through checking cellular ATP levels with a CellTiter-Glo luminescent cell viability assay kit (Promega). The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA from feather pulps and tracheas were extracted using the MagaZorb® DNA mini-prep kit (Promega, Madison, WI), following the manufacturer’s recommendations with a few modiications ...
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bioRxiv - Systems Biology 2024Quote: ... Extracts were used for quantification of luciferase as proxy for ATP content using the ENLITEN ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria were pelleted by centrifugation and DNA extraction was performed with the Wizard® genomic DNA purification kit (Promega).
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bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
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bioRxiv - Cancer Biology 2021Quote: ... the production of IFN-γ in the supernatant was quantitated using an ELISA-ready-go! Kit (eBioscience) and cell pellets were used to measure organoid viability using a CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
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bioRxiv - Immunology 2021Quote: ... Cell supernatants were analyzed for LDH activity using the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega, Cat. no: G1780).
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...