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Citations for Promega :
5051 - 5100 of 5375 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from fBECs of WT and Gpr126iECKO mice at P18 using Maxwell RSC simplyRNA tissue kits with the Maxwell RSC Instruments (Promega Corporation). The amount of RNA was measured using the Nanodrop technique and its integrity assessed using Agilent Bioanalyzer 2100 with Nano RNA kits (RIN > 8) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from fBECs from WT and Gpr126iECKO mice at P18 using Maxwell RSC simplyRNA tissue kits with a Maxwell RSC instrument (Promega Corporation). The amount of RNA was measured using the Nanodrop technique and reverse transcribed with random hexamers (High-Capacity cDNA Archive kits ...
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bioRxiv - Genomics 2022Quote: ... The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega, Madison, USA) with thermal cycling conditions of 2 min at 95 °C ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from a piece of caudal fin using a Maxwell® RSC Blood DNA Kit (Promega, Madison, WI, USA). Extractions were performed following the manufacturer’s instructions with the exception of a longer two-hour lysis step ...
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bioRxiv - Evolutionary Biology 2022Quote: ... H12) of each 96 well plate were pooled prior to DNA extraction using Wizard Genomic DNA purification kit (Promega, Madison, Wisconsin). The P0 region including the beginning of galK was amplified for 25 PCR cycles using primers deep_seq_Fw and deep_seq_Rv carrying 5’ adaptors for Illumina sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... the mycelium was harvested and then used in the RNA extraction following the protocol provided in the Maxwell® 16 LEV simplyRNA Cells Kit (Promega). RNA concentrations were measured by NanoDrop and purified RNA was stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then pelleted and used for genome extraction using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) and Qiaquick PCR purification kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were washed once with 200 µl of dPBS and subsequently analyzed using a Dual-Luciferase Reporter Assay System kit (Promega E1910) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphorothioated and phosphorylated/phosphorothioated forward primers as well as a phosphorylated reverse primer were done in qPCR reaction mixtures including 1X reaction mixture of GoTaq 2-Step RT-qPCR kit (Promega, USA), 400 nM forward and reverse primers and in final volume of 20 μL ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot was used for DNA extraction using the Promega Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: Four to five organoids were physically lysed using a pipette and purified for RNA on the Promega Maxwell RSC48 in conjunction with the Maxwell RSC Simply RNA Tissue kit (Promega, AS1340). cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... 18-24 hours later the supernatant was harvested for cytotoxicity evaluation by measuring LDH release with CytoTox 96 nonradioactive cytotoxicity assay kit (Promega, #G1780), followed the protocol suggested by the manufacturer.
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bioRxiv - Zoology 2022Quote: ... and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells) using CellTox™ Green Cytotoxicity Assay kit (Promega). After the samples were prepared according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Extracellular lactate was measured according to the manufacturer’s protocol using the Lactate-Glo™ Assay kit (J5021, Promega, Leiden, The Netherlands).
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bioRxiv - Molecular Biology 2022Quote: ... and 3μg RNA was used to synthesize the first strand cDNA in a 20μl reaction using the Reverse Transcription System kit (Promega, Beijing, China). The GoTaq® qPCR and RT-qPCR Systems kit (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor DNA was extracted from parts of FFPE containing a high fraction of tumor cells using Maxwell® RSC DNA FFPE Kit (Catalog number: AS1450, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: Total RNA was extracted from powdered whole heart tissue samples using the SV Total RNA isolation kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions or from hiPSC-cardiomyocyte extracts with the TRIzolTM reagent (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: NAD and NADP levels were measured using the commercially available kits NAD/NADH-Glo Assay and NADP/NADPH-Glo Assay (Promega G9071, Promega G9081), respectively ...
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bioRxiv - Genomics 2023Quote: ... control Fiber-seq on dechromatinized DNA was performed on genomic DNA purified from Drosophila S2 cells using the Promega Wizard HMW DNA Extraction Kit (Promega A2920). 2 µg of DNA was used in a final volume of 60 µL in Buffer A with 0.8 mM S-adenosylmethionine and 200 U Hia5 ...
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bioRxiv - Immunology 2024Quote: ... T cell cytotoxicity was assessed by quantification of LDH levels in the supernatants using the LDH-Glo cytotoxicity kit (Promega, J2380) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with increasing concentrations of CMLD012824 and analyzed using a RealTime-Glo Annexin V Apoptosis and Necrosis Assay kit (Promega JA1011) at 1 to 72 h ...
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bioRxiv - Microbiology 2023Quote: ... We used 1 ml of cultures and isolated the genomic DNA using the Wizard Genomic DNA Purification Kit (Promega; Madison, WI). We used the DNA with the Illumina DNA Prep kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Treatments did not affect bacterial or cell viability as assessed by Lactate dehydrogenase release in the culture supernatant (LDH cytotoxicity kit®, Promega, according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Activities of firefly luciferase (LUC) and Renilla luciferase (REN) were examined using a Dual-Luciferase Reporter Assay System kit (Promega, E1960). LUC/REN was calculated as the relative activity and the ratio of dark / HL was used to indicate light response ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates were prepared as per the manufacturers protocol using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega: G7570). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... 3 ml overnight cultures were subjected to DNA extraction using an automated Maxwell® 16 Tissue DNA purification kit and following the manufacturer’s instructions (Promega, UK). For long-read complete genome analysis ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected after 24 hours and luciferase assays were performed using a dual-specific luciferase assay kit (Promega, WI, US) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... 1 µl of duplex DNA was ligated into 25 ng of pBract214m-HvCas9-HSPT vector linearized with AarI using the ligation reagents and protocol described in pGEM®-T Easy Vector System kit (Promega). The ligation reaction was incubated overnight at 4°C then transformed into competent E ...
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bioRxiv - Neuroscience 2023Quote: ... equal amount of RNA (2 μg) from each sample was subjected to cDNA synthesis using reverse transcriptase Kit (Promega Corporation, USA) following standard procedures ...
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bioRxiv - Immunology 2023Quote: Culture supernatants from the different BMDMs samples were analyzed with the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega# G1780). Total cell lysate of non-treated (i.e. ...
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bioRxiv - Cell Biology 2023Quote: Constitutional genomic DNA is extracted from total bone marrow at the remission and purified using magnetic beads (Maxwell® RSC Whole Blood DNA Kit, PROMEGA). Tumor genomic DNA is extracted and purified using magnetic beads (Maxwell® RSC Tissue DNA Kit ...
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bioRxiv - Cell Biology 2023Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... a calibration curve for SAH was constructed in two independent experiments using from 0 to 5 μM SAH according to the manufacturer’s protocol of the MTase-Glo kit (Promega, Wisconsin, USA). The signal was detected using a Tecan Spark microplate reader (Tecan Japan Co. ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pretreated with indicated antagonist for 1hr before treatment with 10 nM of PGF2a or 100 nM PGE2 overnight before glucose uptake was measured by Glucose Uptake-Glo™ Assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... 1 well-1 with 100 μL fresh cell culture media) and mixed with 100 μL reaction solution of CellTiter-Glo 3D cell viability assay kit (G9681; Promega, WI). The 96-well plate was gently rocked for 5 min and stabilized at room temperature for 30 min ...
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bioRxiv - Genomics 2023Quote: ... DNA desulphonation and cleanup steps were immediately performed following the manufacturer instructions of the MethylEdge® Bisulfite Conversion System kit from Promega. After the column cleanup ...
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bioRxiv - Molecular Biology 2023Quote: ... prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat. #E2920). The whole procedure was repeated three times independently for each construct.
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bioRxiv - Biochemistry 2023Quote: ... After 48 h of incubation at 37 °C and 5 % CO2 culture medium was exchanged with fresh growth medium and Luciferase activity was measured with the Dual-Glo® Luciferase Assay Kit (Promega) according to manufactureŕs instructions using the Tecan Spark® plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2023Quote: ... Resulting genomic DNA was assessed for concentration and purity using Promega DNA quantification kit on the Promega Quantus Fluorometer (Promega, Germany) and measurement of A260/A280 ratio on the MultiSkan Sky High spectrophotometer (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
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bioRxiv - Cancer Biology 2023Quote: ... they were treated for the indicated times and viability was assessed using the CellTiter-Glo® 3D Cell viability kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB231 cells were imaged by SEM and quantified by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US). For detection of stem-like properties of cells in scaffolds ...
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bioRxiv - Bioengineering 2023Quote: ... Substrate-dependent differences of cell adhesion and growth were determined by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US) after 5 h and 7 d of culture ...
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bioRxiv - Immunology 2023Quote: Nanoluciferase readings from fecal samples were performed as a proxy for parasite burdens as previously described using the NanoGlo Luciferase reporter assay (NanoGlo Luciferase kit, Promega, N1150) (Vinayak et al. ...