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Citations for Promega :
5251 - 5300 of 5345 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Primer sets for real-time PCR (Table 1) were used to amplify target regions from genomic DNA and cDNA as templates by the GoTaq DNA polymerase (Promega, WI, USA). For real-time PCR experiments ...
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bioRxiv - Neuroscience 2022Quote: ... followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A) at 37°C for overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the media into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Developmental Biology 2024Quote: ... using a spinning disk confocal microscope (see in vivo confocal imaging paragraph above) after embedding the embryos in 1% low-melting agarose (Promega, Cat# V2111) in PBS 1X in a glass bottom 60μ- Dish (35mm high ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of total RNA in combination with oligo(dT) and Improm-II reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed as described previously [55] with SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... measurements 1×105 T cells were cultured in white 96 well plate and analysed using NAD/NADH-Glo™ luminescent assay (Promega, G9071), according to the manufacturer instructions ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... The gradients were centrifuged in an SW41 rotor at 200,000xg for 1hr Fractions were collected from air-gradient interfaces and complemented using LgBiT (Promega, 1:200 dilution) and nanoluc substrate (Promega ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega, Dübendorf, Switzerland).
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bioRxiv - Microbiology 2023Quote: ... Sample digestion was carried out by resuspending beads in 1 µL of trypsin solution (0.2 µg trypsin in Promega V5111 resuspension buffer) and 19 µL 50 mM ammonium bicarbonate and incubating at 37 °C with agitation for 18 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Bioengineering 2023Quote: Mice were administered either 200μL of 15 μg/mL D-luciferin or a 1:40 dilution of Nano-Glo substrate (Promega, diluted in DPBS) by intraperitoneal injection for firefly luciferase and Antares or nanoluciferase imaging ...
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bioRxiv - Genomics 2023Quote: ... traces of genomic DNA were removed by treating 600 ng of total RNA with 1 µl RQ1 RNase-Free DNase (Promega, cat# M6101) in 9 µl reaction additionally containing RQ1 DNase buffer and 1 µl of murine RNase inhibitor at 37°C for 45 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Immunology 2022Quote: ... the buffer was diluted with 50 mM ABC to a final of 2M urea and digested overnight at RT with 1 µg trypsin (Promega, Walldorf, Germany). On the next day ...
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bioRxiv - Biophysics 2023Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 ug in 10 uL of 50 mM TEAB; Promega, Madison, WI). Following this an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2023Quote: ... GAG/POL and VSVG plasmids were co-transfected with transfer plasmid into HEK293Ts or HEK293FTs using FuGENE HD (Promega, 3.33:1 FuGENE:DNA), or lipofectamine 3000 Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... The captured proteins were washed twice in 200 μl of 95% MeCN and transferred to 200 μl of 50 mM ammonium bicarbonate (ABC) containing 1 μg sequencing grade modified trypsin (Promega, Madison, USA) and mixed for 2 hr at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... The first-strand cDNA from a colonic sensory neuron was used as template in a PCR reaction containing 1×GoTaq reaction buffer (Promega Madison, WI), 20 mM outer primers ...
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bioRxiv - Physiology 2024Quote: ... target cDNA fragments were amplified with the primers listed in Table 1 and ligated into pGEM T-Easy vector (Promega, Madison, WI). The cDNA fragments were PCR amplified with M13 primers and purified with the FastGene Gel/PCR Extraction Kit (Nippon Genetics ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated at 37°C for 24 h before proliferation was assayed by Hoechst staining or WST-1 and luciferase signal was measured by Steady-Glo® Luciferase Assay System (Promega, E2520).
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bioRxiv - Biophysics 2024Quote: ... The samples were dried again and rehydrated with 20 µl of trypsin solution with 0.01% ProteaseMAXTM surfactant comprised of 10 ng µl-1 Trypsin (Promega Corp., Madison, WI) in 25 mM NH3HCO3 / 0.01% w/v of ProteaseMAXTM from Promega Corp ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed with TBST and incubated either with anti-mouse or anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (Promega, 1:10000). Chemiluminescence images were captured by the BioRad ChemiDoc detection system.
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bioRxiv - Neuroscience 2024Quote: ... Frozen brain tissue was homogenised in a dounce-homogeniser containing 2 ml ice-cold lysis solution (Nuclei EZ Lysis Buffer [Sigma-Aldrich, NUC101] or Nuclei PURE Lysis buffer [Sigma-Aldrich, NUC201] with 1 mM dithiothreitol [DTT, Promega, P1171 ...
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bioRxiv - Molecular Biology 2024Quote: ... TFs mRNA levels were evaluated by reverse transcription followed by real-time quantitative PCR (RT-qPCR) using the GoTaq 1-Step RT-qPCR System (Promega, Madison, USA) and primers listed in S5 Table ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3X with TBST and incubated with secondary antibody: anti-rabbit IgG HRP conjugate (Promega, catalog no. W4011, 1:20,000), anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3X with TBST and incubated with secondary antibody: anti-rabbit IgG HRP conjugate (Promega, catalog no. W4011, 1:20,000), anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... The mixture was added with 25 mM NH4HCO3 to dilute urea to 1 M followed by overnight digestion with sequencing-grade trypsin (Promega, Madison, WI, USA) at an enzyme/protein ratio of 1:40 (wt/wt ...
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bioRxiv - Immunology 2021Quote: ... the aqueous phase was added to 1 volume of 70% ethanol and loaded on ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 to 2 µg of RNA was used for DNase treatment as per the protocol provided by the manufacturer (Promega, Madison, Wisconsin, USA). This was followed by cDNA synthesis using reverse transcriptase kits from Applied Biosystems (High capacity cDNA synthesis kit ...
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bioRxiv - Molecular Biology 2019Quote: ... and the first-strand cDNA was synthesized from 1 μg of total RNA with oligo dT or random primers using ImProm-II reverse transcriptase (Promega, Madison, WI, USA). The qPCR reactions were performed in a 20 μL reaction mixture using SYBR Green PCR Master Mix (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded into 24-well plate and cultured to 80% confluence when they were co-transfected with 4 μg of either TOP flash or FOP flash vector and 1 μg of pRL-TK (Renilla-TK-luciferase vector, Promega, Madison, WI, USA) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega, Madison, WI, USA). A second incubation with the same amount of trypsin (5 h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... a total of 1□μg of RNA for each sample was reverse-transcribed to cDNA using reverse-transcription kits (Promega, Madison, WI, USA), and real-time quantitative reverse transcription (qRT)-PCR was performed on a 7500 real-time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... To this was added: 1 µl of EcoRV-digested pBlueScript KS II+ (∼100 ng) that had been treated with calf intestinal phosphatase (Promega Corp., Madison, WI), and 0.1 µl of T4 DNA ligase (NEB) ...
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Evolution of sex hormone binding globulins reveals early gene duplication at the root of vertebratesbioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription (RT) was performed using 1 μg of RNA for each sample with M-MLV reverse transcriptase and random hexamers (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Plant Biology 2021Quote: The cDNAs corresponding to the two alleles of SoIAA2 were cloned into a pFN2A (GST) flexi vector (Promega – primers in Supplementary Table 1), expressed in E ...
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bioRxiv - Pathology 2021Quote: ... benthamiana leaves were collected at 48 h after infiltration and then incubated with 1 mM luciferin substrate (catalog no. N1110; Promega Biotech, Beijing, China). LUC images were captured with a Tanon-5200 Multi Chemiluminescent Imaging System (Tanon ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellets were reconstituted and digested with endoproteinase Lys-C (Alpha Laboratories, UK) for 1 hour at room temperature and trypsin (Promega, Madison, WI, USA) overnight at 35°C.
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bioRxiv - Biochemistry 2022Quote: ... transfer plasmid was co-transfected with GAG/POL and VSVG plasmids into HEK293T or 293FT cells using FuGENE HD (Promega, 3.33:1 FuGENE:DNA) and the medium was replaced 6–8 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... blots with α-PRDX6 and α-PfNAPL were incubated with respective α-mouse and α-rabbit horse radish peroxidase (HRP)-coupled secondary antibodies (1:5000, Promega) for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... on the day before transfection and were transfected with 1 μg of plasmid DNA per well using FuGENE-HD reagent (Promega, product number E2312) per manufacturer guidelines ...
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bioRxiv - Developmental Biology 2022Quote: Samples for CaTS-RNAseq were collected in the same way as for CaTS-ATAC but all buffers were supplemented with 1 µL/ml of RNasin Plus (Promega, Cat. No. N2615). After fixation and quenching ...
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bioRxiv - Immunology 2020Quote: ... washed beads were then resuspended in 35 μL of ammonium bicarb buffer to which 1 μL sequencing grade porcine trypsin was added (Promega, Cat. No. V5111). Immune complexes were digested on bead for 1 hr at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... The samples after pull-down assay and co-immunoprecipitation were also digested by 1 µg sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight in the same manner ...
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bioRxiv - Microbiology 2022Quote: ... the aqueous phase was added to 1 volume of 70% ethanol and loaded on ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibody for 30min (anti-mouse IgG HRP conjugate Promega #W402B, anti-rabbit IgG HRP conjugate Promega #W4011, 1:3000) and chemiluminescence was detected using Western Bright ECL spray (Advansta ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized using 1 µg of purified RNA and Promega M-MLV RNase H Minus Point Mutant Reverse Transcriptase (Promega, Madison, WI M3682) using the manufacturer’s recommended protocol for oligo dT-primed synthesis ...
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bioRxiv - Pathology 2020Quote: ... The partial sequence encoding the nucleoprotein from HPWMoV was amplified using specific primers (Table 1) from two-fold diluted cDNA with GoTaq® Flexi DNA polymerase (Promega, WI, USA) with the following reaction conditions ...