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Citations for Promega :
5051 - 5100 of 5345 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Samples containing 20-30 pmol rhodopsin were mixed with 100-250 fmol BSA and cleaved with 1 μg trypsin/LysC mix (Promega) using the SP3 beads protocol described in [29].
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 40,000 cells per well were seeded into poly-D-lysine-coated 96-well plates (Brand, 781965) and the Halo-ligand was added (1:2,000; Promega, G980A). For each transfection ...
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bioRxiv - Microbiology 2023Quote: ... to a final urea concentration ˂ 2 M and then digested with modified trypsin (1:50 w/w) (Sequencing Grade, Promega) at 37°C for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated proteins were digested with 1:50 w/w LysC (Wako Chemicals, cleaves at the carboxylic side of lysine residue) and 1:100 w/w trypsin (Promega, Sequencing-grade ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 in storage buffer and analyzed as indicated in the manufacturer’s protocol (LDH-Glo Cytotoxicity Assay, Promega, #J2381).
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bioRxiv - Molecular Biology 2023Quote: ... The library preparation involved using 100 ng of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA from Promega. Subsequently ...
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bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for 10min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were obtained from 400 ng of RQ1-treated RNA using 1 μl of GoScript Reverse Transcriptase (Cat. No. A5003; Promega) in a 20-μl final volume reaction using random primers (Cat ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Bioengineering 2023Quote: ... tumor fragments (200-300 mg) were lysed for 1 h on ice with agitation in 1X Reporter Cell Lysis Buffer (Promega) and then centrifuged at 14,000 x g for 15 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... 2000 transfected cells were replated into white 384-well tissue culture plates in the presence or absence of 1 μM HaloTag NanoBRET-618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... shRNA-expressing lentiviruses were generated by co-transfecting 293T cells with pLKO-puro.1 vectors and the packaging vectors ΔVPR and VSVG (FuGENE, Promega). Viruses were harvested 24-48 h post transfection ...
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Strap binding buffer was applied to precipitate proteins on quartz and proteolysis took place during 14 hrs at 37°C with 1 µg Trypsin sequencing grade (Promega). After speed-vacuum drying of eluted peptides ...
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bioRxiv - Systems Biology 2023Quote: ... The urea in the lysis buffer was then diluted to a final concentration of 1 M with 50 mM ammonium bicarbonate followed by trypsin digestion (Promega) with enzyme ...
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bioRxiv - Cancer Biology 2023Quote: ... we transfected 500,000 ANBL6 cells with 1 ug of reporter plasmid plus 250 ng of Rinella plasmids pRL-SV40 and pGL3 control (Promega) in biological triplicates ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were revealed using species-appropriate HRP-conjugated secondary antibodies (anti-rabbit or anti-mouse IgG (1:5000, Promega), or anti-guinea pig IgG (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with 1× PBS and lysed with PLB according to the manufacturer’s instruction for 15 mins (Promega, E1910). Cells were transferred to a 96-well plate ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated RNAs (20 ng) were reverse-transcribed and quantitative PCR (qPCR) were perform using GoTaq Probe 1-Step RT-PCR system (Promega) in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Blots were washed twice with 1X PBS-T followed by incubation for 1 hour with HRP-conjugated secondary antibody (Promega) diluted 1:10,000 in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed twice with 1X PBS-T followed by incubation for 1 hour with HRP-conjugated secondary antibody (Promega) diluted 1:10,000 in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... packed column (now loaded with the protein sample) 20 µL of Trypsin digestion solution (1 µg MS-grade Trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of total RNA was incubated with RNase-free DNase I (1 unit/μl) following the protocol provided by the manufacturer (Promega), followed by reverse-transcription into first-strand cDNA using ImProm II reverse transcriptase (Promega) ...
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bioRxiv - Systems Biology 2023Quote: ... 37.5 µL of 8 M Urea and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... prepared by adding NanoDLR™ Stop & Glo® Substrate 1:100 into NanoDLR™ Stop & Glo® Buffer (Promega, USA), were added to the samples ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5 mM of dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... Culture aliquots (200 µL) were taken to measure OD620 and luminescence by adding 10 µL of 1 mg/mL beetle luciferin (Promega) in a BioTek Cytation 3 Microplate Reader ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed with 100 μL PBS and lysed by addition of 20 μL 1 x Passive Lysis Buffer (Promega) to each well and nutated for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected with pCI-neo-hsSART1 full-length and ideletion mutants with 1 ng/μl using ViaFect Transfection Reagent (Promega). After 4 h incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested with 1 μg of lysyl endopeptidase (Wako) at room temperature overnight and were further digested overnight at room temperature with 1 μg trypsin (Promega). The resulting peptides were desalted with an HLB column (Waters ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 49 ng of a TP1-Luciferase (Kurooka et al., 1998; Minoguchi et al., 1997) and 1 ng Renilla-Luciferase (pRL-TK, Promega) using Lipofectamine™ 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatant was diluted at 1:50 with PBS and used for luminescence measurement with the Nano-Glo Luciferase Assay System (cat. #N1110, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Biochemistry 2023Quote: ... The captured proteins were washed twice in 200μl 95% MeCN and transferred to 200μl 25mM ammonium bicarbonate containing 1 μg sequencing grade modified trypsin (Promega, Madison, USA) and mixed for 4 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours followed by another overnight digestion with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... core and modified histones and transcription factors at the HIV-1 LTR was assessed by quantitative PCR using primers spanning the full promoter (Table 1) with GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by a digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
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bioRxiv - Plant Biology 2024Quote: ... in presence of 40 U of RNase OUT (both from Invitrogen, using ∼1 μg of total RNA and 100 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50 °C ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of cell lysates were diluted 1:30 and assessed for firefly and Renilla luciferase activity using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Trypsin was reconstituted in 50 mM acetic acid in water at a concentration of 1 μg/μL (Trypsin Gold, Promega). Reconstituted trypsin was added to 50 mM NH4HCO3 in a 1:50 ratio (v/v) ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell-cell-cell fusion was performed by measuring luciferase expression as relative luminescence units (RLU) 1 min by mixing cell lysates with the Steady-Glo luciferase substrate (E2520, Promega) on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was then reverse transcribed by extension of random primers using M-MLV (Promega, Madison,WI). Relative expression levels were determined by real-time PCR (qPCR ...
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bioRxiv - Immunology 2024Quote: ... Blots were incubated with primary antibodies overnight at 4°C followed by 2-hour incubation with HRP-conjugated secondary anti-rabbit IgG-HRP conjugate antibodies (1:10,000; Promega W401B) at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 min) and diluted to 1M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8h, Promega). After digestion ...
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bioRxiv - Microbiology 2024Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Secondary antibodies were diluted in blocking buffer and incubated with the membrane for 60 min at room temperature (1:10,000 anti-rabbit-HRP, Promega #W401B). After secondary antibody incubation ...