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Citations for Promega :
5101 - 5150 of 5345 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 40 mM ammonium bicarbonate and digested by 1 ug sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight ...
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bioRxiv - Plant Biology 2020Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Plant Biology 2019Quote: ... An aliquot containing 4 μg RNA was treated for 45 min with 2 μl of DNase RQ1 (1 μg/μl) at 37 °C (Promega, UK), and purified using an RNAeasy spin column (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
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bioRxiv - Cell Biology 2020Quote: ... Heterologous expression of proteins was achieved by transfecting cells with 1 □g of plasmid DNA/35 mm using FuGENE HD (Promega; E2311), as previously described (Webb et al. ...
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bioRxiv - Cell Biology 2019Quote: ... cat # 129-02541) overnight at 37°C and then diluted 1/2 and digested with 0.9 µg of trypsin (Promega, cat # V5113) for eight hours at 37°C ...
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bioRxiv - Genetics 2021Quote: ... cell supernatant from untransfected and cells transfected with IL2 constructs was diluted 1:12 with complete media and used to perform the IL2 Bioassay (Promega, JA2201). Luminescence (RLU ...
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... and then resuspended in tagmentation reaction mix for 30 min (2.5uL TD1, 1X TD Buffer, Illumina Nextera DNA, FC-121-1030; .25uL 1% digitonin, Promega G9441; 1xPBS;) gently shaking (300rpm on Eppendorf thermomixer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113).
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bioRxiv - Molecular Biology 2020Quote: ... DF-1 cells were transfected with RCASBP-(A)-β2m-sgRNA or MOCK RCASBP-(A)-EGFP-sgRNA using ViaFectTM Transfection Reagent (Promega, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified PCR-amplified promoter fragments (∼1 kb) were cloned between KpnI and XhoI sites in firefly luciferase reporter vector pGL3-Basic (Promega, USA). The resulting plasmids were named as SHR-Hmgcr and WKY-Hmgcr which contain −985 bp to +20 bp region of SHR and WKY Hmgcr promoter sequence ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesised using 1 µg of total RNA in a 20 µL final volume using the GoScript Reverse Transcription System (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... 20×103 cells were seeded in a 24-well plate and co-transfected using PEI with pGL3-Chaserr-reporter vector (100 ng) and psiCHECK-1 (100 ng, Promega, C8011). Firefly and Renilla expression were measured using Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2021Quote: Around 1×105 cells were treated as indicated prior to lysis and RNA isolation using the ReliaPrep™ RNA Miniprep kit (Promega). Approximately 1μg of total RNA was reverse transcribed into cDNA using SuperScript III (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... senders were stained at a density of 1.00E+07 cells/mL on ice for five minutes in the presence of either 1 µM HaloTag-Alexa Fluor 488 (Promega G1001) or 3.5 µM HaloTag-Alexa Fluor 660 (Promega G8471) ...
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bioRxiv - Biochemistry 2021Quote: ... WT or ADAR1-KO HEK293T cells in 12-well plates were transfected with 400 ng ml-1 of firefly luciferase under control of the IFN-β promoter (pIFN-Luc, Promega), 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK ...
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bioRxiv - Plant Biology 2020Quote: ... using 1 µg of total Arabidopsis or cauliflower mtRNA and 250 ng of a random hexanucleotide mixture (Promega, Cat no. C1181) and incubated for 50 min at 50°C ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well and incubated for 1 h at RT before fluorescence intensity was read on a Glomax® Explorer Fully Loaded (Promega) with emission wavelengths of 500-550 nm and an excitation wavelength of 475 nm and graphs were generated using GraphPad Prism (V.8.4.1) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then alkylated using 15mM chloroacetamide at 37°C for 30min and further digested using sequencing-grade modified trypsin (1/25, w/w, trypsin/protein; Promega, USA) at 37°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein pellet was resuspended in 190 ul of 50 mM ammonium bicarbonate and digested with 20 ul of 1 ug/ul trypsin gold (Promega V5280) at 37°C for 4 hours.
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
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bioRxiv - Cell Biology 2020Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113). After digestion ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was separated on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System (Promega, USA).
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% sodium deoxycholate (protect from light), 1:200 Protease Inhibitor Cocktail III), and then, incubated with RNase I (Life Technology, AM2295) and DNase (Promega, M6101) in a Thermomixer at 1200 rpm to fragment RNA at 37 °C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: Peptide mass spectrometry was completed after reduction with 10 mM DTT and alkylation with 55 mM iodoacetamide and protein digestion overnight at 37 °C at a 1:50 ratio of trypsin (Promega, UK). Tryptic peptides were analysed by nano-scale capillary LC-MS/MS with an Ultimate U3000 HPLC (ThermoFisher Scientific Dionex ...
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bioRxiv - Molecular Biology 2022Quote: ... or 1/6th of the diaphragm) using a Maxwell RSC Instrument and Maxwell RSC simplyRNA Tissue Kit (both Promega, Southampton, UK) and complementary DNA (cDNA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Cancer Biology 2022Quote: Cell pellets were thawed in Lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 1% NP-40 supplemented with protease inhibitor cocktail (Promega cat #G6521)) and incubated on ice for 15min ...
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bioRxiv - Cancer Biology 2022Quote: ... Pellets were thawed with lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 1% Triton X100, 1mg/ml Na-deoxycholate) supplemented with protease inhibitor (Promega cat #G6521). The bait is covalently bound through HaloTag to the beads and therefore cannot be detected by blotting.
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bioRxiv - Neuroscience 2020Quote: ... total RNA was placed in 4 µl of lysis buffer (0.1% vol/vol Triton X-100, 2.5 mM dNTPs, 2.5 µM oligo-dT, 1 U/µl Promega RNasin Plus RNase inhibitor). Reverse transcription was performed followed by cDNA amplification ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Cell Biology 2021Quote: ... and 40 μg of proteins/sample was loaded onto S-trap columns (Protifi) and digested overnight with 1 μg sequencing grade modified trypsin (Promega, V5111) at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested overnight at room temperature with a 1:50 ratio of sequencing grade modified trypsin (0.4ug, V511A, Promega, Fitchburg WI) in 130ul TEAB (0.05M Triethylammoniumbicarbonate in water) ...
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bioRxiv - Biochemistry 2021Quote: ... and the “on-bead-digestion” plate (100 μl 20 mM Tris pH 8.5, Sigma-Aldrich 10708976001, 1 μg/mL LysC, Wako 129-02541, 2 μg/mL Trypsin, Promega V5111). The programmed sequence is ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA was added to the 100 µl SA Sepharose slurry together with 1 µl RNasin Plus RNase inhibitor (40 U/µL, Promega, N261A). 10 µl of the supernatant was saved for extraction of input RNA using TRIzol (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... then diluted 1:5 with nuclease-free water before 2 µL was used as template in a 10 µL GoTaq 1-Step RT-qPCR (Promega, A6020) reaction (69) ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 1 M with 50 mM ABC and the proteins digested with trypsin (Promega, V511c) at 1:100 mass-ratio at 37 °C overnight.
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bioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the ORF8 plasmid together with the transposase coding plasmid (1/10) with Fugene HD (Promega, Germany) and after one day selected with 3 µg/ml puromycin for three days ...
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bioRxiv - Cancer Biology 2022Quote: ... 20µg of chromatin was subjected to buffer exchange through SP3 protein clean up protocol (87) and resuspended in AmBic-10mM DTT before proteins digestion with 1:50 trypsin (Promega V5280) for 18h ...
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bioRxiv - Systems Biology 2019Quote: ... The samples were then diluted with 0.1 M NH4HCO3 to a final concentration of 1 M urea and the proteins were digested with sequencing-grade porcine trypsin (Promega, Switzerland) at a final enzyme:substrate ratio of 1:100 (w/w) ...
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunofluorescence staining: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:500 dilution), mouse monoclonal antibody to lamin A/C (Cell Signaling ...
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bioRxiv - Physiology 2020Quote: ... Larvae with intestinal tracts clearly outlined with Nile Red dye were embedded in 1% low melting point agarose (Promega, Madison, WI) in E3 supplemented with 0.16 mg/mL tricaine in 6-well glass bottom plates (#1.5 high performance cover glass ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.6 or 1 μg extracted RNA was converted into cDNA by reverse transcription using a High Capacity cDNA Reverse Transcription kit (Promega A3500) or a ReverTra Ace qPCR RT Master Mix (Toyobo) ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL aliquots of phage lysate with a titer of approximately 109 PFU mL-1 was digested with DNase I (Thermo, CA, USA) and RNase A (Promega, USA) to remove any residual bacterial genomic DNA and RNA according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... using 1 μg of total Arabidopsis or cauliflower mtRNA and 250 ng of a random hexanucleotide mixture (Promega, Cat no. C1181) and incubated for 50 min at 50°C ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription of viral RNA followed by quantitative PCR was performed with GoTaq® Probe 1-Step RT-qPCR System (Promega) on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplification reactions were run in a 20 μL volume using the GoTaq 1-Step RT-qPCR System (Promega, Madison WI, USA) and the recommended manufacturer’s instructions as described in Cervera et al ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μl) were mixed with the master mixes containing 1 μl primers (10 μM each) and reagents of GoTaq G2 (Promega #M7845) and the final volume was 25 μl ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with TBS-T and imaged using 500 µl of 1:500 NanoGlo substrate (Promega, catalogue number: N1120) diluted in 10 mM sodium phosphate buffer pH 7.0.