Labshake search
Citations for Promega :
5101 - 5150 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: Cell lysates were prepared as per the manufacturers protocol using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega: G7570). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... 3 ml overnight cultures were subjected to DNA extraction using an automated Maxwell® 16 Tissue DNA purification kit and following the manufacturer’s instructions (Promega, UK). For long-read complete genome analysis ...
-
bioRxiv - Biochemistry 2023Quote: ... After 48 h of incubation at 37 °C and 5 % CO2 culture medium was exchanged with fresh growth medium and Luciferase activity was measured with the Dual-Glo® Luciferase Assay Kit (Promega) according to manufactureŕs instructions using the Tecan Spark® plate reader ...
-
bioRxiv - Biochemistry 2023Quote: ... a calibration curve for SAH was constructed in two independent experiments using from 0 to 5 μM SAH according to the manufacturer’s protocol of the MTase-Glo kit (Promega, Wisconsin, USA). The signal was detected using a Tecan Spark microplate reader (Tecan Japan Co. ...
-
bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
-
bioRxiv - Bioengineering 2023Quote: ... 1 well-1 with 100 μL fresh cell culture media) and mixed with 100 μL reaction solution of CellTiter-Glo 3D cell viability assay kit (G9681; Promega, WI). The 96-well plate was gently rocked for 5 min and stabilized at room temperature for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were pretreated with indicated antagonist for 1hr before treatment with 10 nM of PGF2a or 100 nM PGE2 overnight before glucose uptake was measured by Glucose Uptake-Glo™ Assay kit (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
-
bioRxiv - Immunology 2023Quote: Culture supernatants from the different BMDMs samples were analyzed with the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega# G1780). Total cell lysate of non-treated (i.e. ...
-
bioRxiv - Genomics 2023Quote: ... DNA desulphonation and cleanup steps were immediately performed following the manufacturer instructions of the MethylEdge® Bisulfite Conversion System kit from Promega. After the column cleanup ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat. #E2920). The whole procedure was repeated three times independently for each construct.
-
bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
-
bioRxiv - Cancer Biology 2023Quote: ... they were treated for the indicated times and viability was assessed using the CellTiter-Glo® 3D Cell viability kit (Promega), following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... MDA-MB231 cells were imaged by SEM and quantified by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US). For detection of stem-like properties of cells in scaffolds ...
-
bioRxiv - Bioengineering 2023Quote: ... Substrate-dependent differences of cell adhesion and growth were determined by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US) after 5 h and 7 d of culture ...
-
bioRxiv - Immunology 2023Quote: Nanoluciferase readings from fecal samples were performed as a proxy for parasite burdens as previously described using the NanoGlo Luciferase reporter assay (NanoGlo Luciferase kit, Promega, N1150) (Vinayak et al. ...
-
bioRxiv - Microbiology 2023Quote: DNA extractions for genomic sequencing were performed using Maxwell™ RSC Cell DNA Purification Kit and automated extraction on the Maxwell RSC 48 Instrument (Promega). The DNA concentration was evaluated using a Qubit dsDNA BR assay kit and Qubit Fluorometer (ThermoFisher) ...
-
bioRxiv - Genomics 2023Quote: ... leaves and fruits were collected and in the field and total RNA was extracted with Maxwell® RSC Plant RNA Kit (Promega). Two pools were generated and used for PacBio Iso-Seq library preparation (Procedure & Checklist – Iso-Seq™ Express Template Preparation for Sequel® and Sequel II Systems ...
-
bioRxiv - Developmental Biology 2023Quote: ... Targeted sequence of each single clone was validated by Sanger sequencing after gDNA isolation through Wizard® Genomic DNA Purification Kit (#A1120, Promega). For Prkaa1 knockout ...
-
bioRxiv - Cancer Biology 2023Quote: ... Two replicate electroporations were each divided into three wells for 48h of culture and readout using the Dual-Glo Luciferase kit (Promega; E2940) in a Bio-Tek Synergy HT Microplate Reader.
-
bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
-
bioRxiv - Neuroscience 2023Quote: ... equal amount of RNA (2 μg) from each sample was subjected to cDNA synthesis using reverse transcriptase Kit (Promega Corporation, USA) following standard procedures ...
-
bioRxiv - Genomics 2023Quote: ... Resulting genomic DNA was assessed for concentration and purity using Promega DNA quantification kit on the Promega Quantus Fluorometer (Promega, Germany) and measurement of A260/A280 ratio on the MultiSkan Sky High spectrophotometer (Thermo Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Cell viability was assessed continuously based on ATP production with the RealTime-GloTM MT Cell Viability Assay kit (Promega, cat# G9711) through bioluminescence output using the TECAN Spark plate reader.
-
bioRxiv - Cell Biology 2023Quote: Production of hydrogen peroxide (H2O2) in WT and Alas1 KO brown adipocytes was measured using the ROS-GloTM H2O2 assay kit (Promega #G8820) as per manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... NAD+ and NADH were measured using a protocol adapted from the manual of the NAD+/NADH-Glo Assay Kit (Promega, G9072), as previously described (Li et al. ...
-
bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
-
Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: We observed induction of the chromosomal plcH-nLuc-plcR reporter in nanoluciferase reporter assays using the Nano-Glo® Luciferase Assay kit (Promega). The assay was conducted as described by the manufacturer with some modifications reported here ...
-
bioRxiv - Cancer Biology 2023Quote: ... cytotoxicity and apoptosis were measured 48h and 7 days after the knock-down using ApoTox-Glo triplex assay kit (Promega,G6320) according to manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from single cell suspensions of thymic leukemias using the Wizard Genomic DNA Purification Kit (Promega, Madison WI). PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio ...
-
bioRxiv - Cell Biology 2023Quote: Constitutional genomic DNA is extracted from total bone marrow at the remission and purified using magnetic beads (Maxwell® RSC Whole Blood DNA Kit, PROMEGA). Tumor genomic DNA is extracted and purified using magnetic beads (Maxwell® RSC Tissue DNA Kit ...
-
bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
-
bioRxiv - Molecular Biology 2024Quote: ... ATRX 5ʹ-TGAAACTTCATTTTCAACCAAATGCTC-3ʹ and 5ʹ-ATCAAGGGGATGGCAGCAG-3ʹ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... The BSP amplification product was purified by Vazyme Biotech (China) amplification product purification kit and cloned into pGEM-Tasy-vector system (Promega, USA). and the percentages of methylated cytosines over total cytosines were calculated.
-
bioRxiv - Microbiology 2024Quote: ... Cells were processed at different times post inoculation (pi) according to manufacturer’s instructions (Caspase-Glo® 3/7 Assay kit, Promega, USA) to determine caspase 3/7 activity ...
-
bioRxiv - Microbiology 2024Quote: ... and tryptone (Becton Dickinson DifcoTM,)) overnight at 35°C and genomic DNA was extracted using the Wizard® Genomic DNA purification kit (Promega) as previously described [35] ...
-
bioRxiv - Physiology 2024Quote: Total RNA was isolated from snap frozen LV wall or kidney after mechanical grinding (Precellys Evolution, Bertin Technologies, France) using the Maxwell RSC microRNA Tissue kit (Promega, USA). RNA was quantified via NanoDrop (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from 10 ⨯ 10 µm FFPE tissue sections using the Maxwell RSC FFPE RNA kit in accordance with the manufacturer’s manual (Promega, United States). RNA concentration was determined using a Qubit 3.0 Fluorometer using the Qubit RNA BR Assay Kit (Thermo Fischer Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Larvae from progenies with low homing rates of dsxFGFP-null were pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check for the presence of mutations that could have prevented the homing ...
-
bioRxiv - Cancer Biology 2021Quote: ... at 1:166 to Opti-MEM media in combination with extracellular NanoLuc Inhibitor (Promega) diluted 1:500 was combined to create a 3X stock solution ...
-
bioRxiv - Cell Biology 2020Quote: ... We used the following primary antibodies: mouse anti-β-galactosidase (Promega, 1:1,000 dilution), rabbit anti-GFP (MBL,1:1,000 dilution) ...
-
The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... 100 ng of Trypsin Gold and 1 μg of Trypsin sequenced grade (both Promega) were added to BioID eluates and total cell lysates ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and in bioluminescence mode while immersed in NanoGlo substrate (diluted 1:50, Promega #N1110). Tissues were homogenized in 1 ml N-PER (brain ...
-
bioRxiv - Cancer Biology 2022Quote: ... After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:3,000, Promega), protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore ...
-
bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested in 1 M urea with modified trypsin (Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were then harvested and lysed with 25μL of 1×passive lysis buffer (Promega) for 30 min at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 1 µg DNA and 3 µl FuGENE HD (Promega, E2311) in 150 µl Opti-MEM media according to manufacturer’s protocol ...