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Citations for Sartorius :
151 - 200 of 536 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Protein-containing fractions were combined and concentrated to 0.5 ml using a 50-kDa molecular weight cutoff (MWCO) Vivaspin 2 concentrator (Sartorius), and desalted by buffer exchange on a PD MiniTrap G-25 column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
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bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
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bioRxiv - Bioengineering 2022Quote: Fed-batch bioreactor experiments were performed in 2-L bench top glass fermenters (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated EVs were subsequently concentrated to a volume of 100 ul using a 2 ml 100,000 kDa Vivaspin column (Sartorius).
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bioRxiv - Immunology 2021Quote: ... Infections were monitored in live cells via GFP expression using the Incucyte live-cell analysis system (Sartorius) for 24-72h ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... cells were treated with Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling (Sartorius, 4717) at time of experiment seeding for a final concentration of 1:750 ...
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bioRxiv - Cell Biology 2019Quote: ... Some of the cell expansion experiments were performed using the automated CompacT SelecT cell culture system (Sartorius). Enzymatic cell dissociation was performed using Accutase (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The serum-free suspended cells were cultured in microbioreactor (Ambr® 15 cell culture bioreactor; Sartorius, Germany) to determine the specific productivity (Qp ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then seeded in 96-well plates in cell growth media containing Annexin V Dye (Sartorius). Cells were imaged by real-time fluorescence microscopy (IncuCyte ...
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bioRxiv - Cell Biology 2024Quote: ... and the cells were subsequently imaged over a timecourse using an Incucyte S3 Live-Cell imager (Sartorius). Pyroptosis was measured based on a cell-by-cell classification analysis that quantified the percentage of green positive cells out of the total number of cells imaged.
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bioRxiv - Molecular Biology 2024Quote: Cell death assessment was performed using the Sartorius IncuCyte S3 live-cell analysis equipment (Sartorius, Göttingen, Germany) housed at the Indiana University School of Medicine ...
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bioRxiv - Cancer Biology 2022Quote: ... using and the pHrodo-stained B cells (IncuCyte® pHrodo® Red Cell Labelling Kit for Phagocytosis, Sartorius). Following phagocytosis assay ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Cells were transduced with Incucyte® Cell Cycle Green/Red Lentivirus Reagent (EF1α-Puro) (Sartorius, Cat. No. 4779) as recommended by the manufacturer and transduced cells were selected by puromycin treatment ...
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bioRxiv - Biochemistry 2023Quote: ... At each of the specified time points cell viability was measured using XTT-based Cell Proliferation Kit (Sartorius), according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... media was changed and live-cell timelapse microscopy of treated cells was performed in an Incucyte SX5 (Sartorius). Images were taken every 30 minutes in the phase ...
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bioRxiv - Cancer Biology 2023Quote: ... cell confluency was determined every 4 hours using live cell imaging system Incucyte® S3 (Sartorius, Göttingen, Germany) and plotted relative to initial values.
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bioRxiv - Cancer Biology 2024Quote: ... Cell numbers were counted before treatment and 24h after treatment using Incucyte® Live-Cell Analysis System (Sartorius) that automatically counts cells based on dedicated analysis adapted on specific cell lines morphology ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell growth was monitored for up to 72hrs using automated Incucyte S3 live cell analysis system (Sartorius, Germany). Phase contrast images were captured every 3hrs in 4x magnification throughout the incubation time ...
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bioRxiv - Cancer Biology 2023Quote: Confluency analysis of GBM1 UbC-GFP cells was performed using the ZOOM Incucyte Live-Cell imaging platform (Sartorius). 8K cells per square centimetre per well were plated in 6-well plates and whole-well imaging was performed in 120-minute intervals using a Nikon 4x objective ...
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bioRxiv - Biochemistry 2023Quote: ... Directly after ligand stimulation the cells were placed in the IncuCyte S3 live-cell imaging analysis system (Sartorius). The cells were imaged every 3 h for a period of 48 h ...
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bioRxiv - Immunology 2024Quote: Real-time cell-death assays were performed using a two-color IncuCyte S3 Live-Cell Analysis instrument (Sartorius). Cell lines were seeded in 12 well plates and treated with cell death stimulating agents or infected with viruses ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell monolayer was scraped and migration was monitored via time-lapse image acquisition every 2 hours via the IncuCyte ZOOM imaging platform (Sartorius). Migration rates were calculated as relative wound density over time for wildtype and mutant cell lines ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... Fragmented gDNA was obtained by beat-beating the intact gDNA for 12 minutes using 2 mm diameter glass beads in a Mikro-Dismembrator U (Sartorius) at 2000 rpm ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Immunology 2020Quote: ... The EV pellet was resuspended in 2 ml PBS and stored at −80 prior to further concentration using a 5 kDa MW cut-off vivaspin (Sartorius). The protein content of EVs was quantified using Qubit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Isolated ECs were seeded onto 96 well-plates pre-coated with fibronectin in EGM-2 and the proliferation assay was performed for 60 hours in the Incucyte proliferation assay according to (21) (Sartorius). The analysis of the cell proliferation was performed by the Incucyte Basic Analyzer software.
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bioRxiv - Bioengineering 2023Quote: ... This experiment was carried out in a commercial stirred tank bioreactor (STBR) of 2 l total volume (Sartorius AG, Germany) and a working volume of 1 l was used ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Cell Biology 2023Quote: ... The remaining vesicle pellets were resuspended in 2 mL sterile 1× PBS and sterile filtered with 0.20 µm syringe filters (Carl Roth, Sartorius VS0271).
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bioRxiv - Synthetic Biology 2023Quote: ... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
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bioRxiv - Synthetic Biology 2023Quote: ... One image per well was acquired at 4x magnification in the green channel (100 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hr using an Incucyte (Sartorius).
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bioRxiv - Immunology 2024Quote: ... Biotinylated OspA (5 µg/mL) in PBS containing 2% w/v BSA was captured onto streptavidin biosensors (#18-5019, Sartorius) for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: IncuCyte ZOOM Live-Cell Imaging system (Sartorius) was used for kinetic monitoring of cell proliferation and cytotoxicity of TLR agonists in murine pancreatic cancer cells ...
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bioRxiv - Bioengineering 2023Quote: ... MCF7 cells were grown in RPMI (Sartorius) supplemented with 4mM Glutamine (Sartorius) ...
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bioRxiv - Cell Biology 2023Quote: The XTT cell proliferation kit (BioInd, SARTORIUS) was performed according to the manufactural protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated in Incucyte Zoom (Sartorius) for 24 hours and the images of the cells were taken immediately and every 2 hours using Incucyte Zoom with a 10X objective.
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bioRxiv - Biochemistry 2023Quote: ... Cells were imaged using Incucyte S3 (Sartorius) every 8 hours for the next 120-140 hours ...
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bioRxiv - Microbiology 2023Quote: ... Total green cell count was measured every 6 h using an Incucyte Zoom S3 live cell imaging system (Sartorius) and normalised for confluency variation ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of PI positive or SYTOX green positive cells were imaged using Incucyte Live-Cell Analysis Systems (Sartorius) for every 30min to 4hrs.
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bioRxiv - Microbiology 2024Quote: U2OS-53PB1-GFP and U2OS-NBS1-GFP cells were imaged using the IncuCyte S3 Live-Cell Analysis Instrument (Sartorius). Mean Fluorescence Intensity (MFI ...
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bioRxiv - Microbiology 2020Quote: ... 1% (w/v) polyvinylpyrrolidone and 2% (w/v) sodium dodecyl sulfate after which 200 mg glass beads (0.11 mm Sartorius, Gottingen, Germany) were added and the cells were lysed for 5 min at 2000 rpm in a FastPrep VR-96 instrument (MP Biomedicals ...
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bioRxiv - Biochemistry 2022Quote: ... A template with a biotin-TEG at the 5’-end was annealed to the primers (Table 2) and immobilized on a streptavidin-coated biosensor (SAX, Sartorius AG). Primers were added at two-fold molar excess over the template ...
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The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identificationbioRxiv - Cell Biology 2019Quote: ... 50 µl media with 2 x compound concentration was added and plates were subsequently placed and monitored in an IncuCyte® (Sartorius) instrument ...
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bioRxiv - Microbiology 2020Quote: Batch and fed-batch bioreactor experiments were performed in 2 L bench top glass fermentors (Biostat B, Sartorius Stedim, Göttingen, Germany) equipped with two 6-blade Rushton impellers ...
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bioRxiv - Plant Biology 2023Quote: ... 250 mM imidazole) and concentrated to 1-2 mL by ultrafiltration using a Vivaspin® Turbo Centrifugal Concentrator (Sartorius, United Kingdom). To remove imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... as well as phase) were taken every 2 h over a period of 72 h using the IncuCyte® (Sartorius, Germany). General macroautophagy flux was calculated via the ratio of the total integrated fluorescence intensities of GFP/RFP ...
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bioRxiv - Plant Biology 2024Quote: ... Fractions were pooled and concentrated to a final concentration of 2 mg/ml using a centrifugal filter device (Sartorius, Göttingen, Germany) with a molecular weight cut-off of 50 kDa.
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bioRxiv - Immunology 2021Quote: ... cell entry was detected through the expression of GFP by visualisation on an Incucyte S3 live cell imaging system (Sartorius). The percent of cell entry was quantified as GFP positive areas of cells over the total area covered by cells ...
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bioRxiv - Cell Biology 2021Quote: Live cell tracking of A549 cells expressing the mVenus-Gem1 reporter was carried out using the IncuCyte live cell imaging system (Sartorius). The cells were plated at 40,000 cells/well density on 6-well plates and the 4-day long chemical treatments were started 24 hours after plating ...