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Citations for Sartorius :
451 - 500 of 536 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The A549/pr(ISRE).eGFP.A1 sub-clone was found to induce high and homogenous levels of eGFP following IFN-α2 stimulation as assessed by the IncuCyte live-cell analysis system (Sartorius) and flow cytometry (see below) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with PBS and incubated in growth medium at 37°C in an IncuCyte S3 Live-Cell Analysis System (Sartorius). At the indicated times post infection ...
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bioRxiv - Microbiology 2022Quote: ... Virus replication was assessed at 24 hpi by quantifying the average red fluorescence in each well using a live cell microscope (Inncucyte S3, Sartorius).
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bioRxiv - Cell Biology 2022Quote: ... individual frames of cells growing under normal culture conditions were acquired every 5 minutes for 24 hours using an IncuCyte ZOOM live-cell analysis system (Sartorius) equipped with a 20 x objective.
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was recorded for up to 7 days every 4 hours by the IncuCyte S3 Live-Cell Analysis System (Sartorius) with a phase-contrast microscope at 10X magnification ...
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bioRxiv - Neuroscience 2020Quote: ... were imaged for fluorescent pHrodo-labelled tau protein (excitation at 577nm and emission at 641nm in an acidic environment) using a 20× IncuCyte S3 Live-cell Analysis System (Sartorius). Bright-field and fluorescence emission images were collected at 45min or 1hr intervals for 16hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP-NANOG MDA-MB-231 cells were plated on fibronectin-coated (30 μg/mL) glass 96 well plates and placed in an Incucyte S3 (Sartorius) live cell imaging system ...
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bioRxiv - Cancer Biology 2019Quote: TNBC control and gene knocked down cells (500/well) were seeded in 384 well plate and transferred to Incucyte ZOOM analysis system (Sartorius) that was maintained at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were grown on a 96-well plate and placed in the incubated chamber of an Incucyte ZOOM Microscope (Sartorius). EpiLC differentiation medium was added and supplemented with 5µM of Caspase 3/7 Green dye (Sartorius ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 104 cells were seeded in each well of an Incucyte® ImageLock 96-well plate (Sartorius, Catalog #: 4379) in 100 μL/well of either complete RPMI or DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... A wound was made in the cell monolayer using a 96-well wound-maker tool with PTFE pin tips (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The fixative was washed off with flow cytometry buffer and cells resuspended in 25 μL of that buffer and analyzed on an iQue Screener PLUS cytometer (Sartorius).
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bioRxiv - Immunology 2022Quote: ... complete media was then layered on top for imaging with an Incucyte S3 live-cell analysis system (Sartorius, Göttingen, Germany). Imaging was performed every hour at 4X magnification using red ...
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Synthetic Biology 2023Quote: ... The preculture cells were then inoculated at initial OD600= 0.1 in triplicates in 1L Biostat Qplus bioreactors (Sartorius Stedim Biotech). The bioreactor cultivations were performed in 500 mL of SDG medium containing 20 g/L galactose (separately autoclaved) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were imaged every 12 h and cell growth and green fluorescence metrics were extracted and analyzed using the IncuCyte ZOOM software (Sartorius).
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bioRxiv - Biochemistry 2022Quote: The bioreactor cell culture experiments were conducted in a 3L glass bioreactor using Biostat B-DCU controller (Sartorius, Göttingen, Germany) with a working volume of 1.75L ...
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bioRxiv - Immunology 2023Quote: HeLa and MDA-MB-231 WT and ARPC5-KO cells were seeded in a 96-well ImageLock plate (Sartorius #4806) and incubated for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... monoclonal antibodies or VHHs were added to transfected cells at a final concentration of 1 µg/ml and GFP expression was monitored hourly over time with an Incucyte Zoom live cell analysis device (Sartorius) and analyzed with the provided Incucyte software.
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Microbiology 2023Quote: ... Plates of infected macrophages were imaged in triplicate per well in an Incucyte S3 Live-Cell Analysis Platform (Sartorius #4647). Phase and fluorescence images were collected every hour per well using a 20X objective ...
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... before percent of GFP-positive cells was determined by high-throughput flow cytometry (Intellicyt iQue Screener PLUS, Sartorius, Gottingen, Germany).
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated at the indicated temperature and recovery was monitored by examination of GFP expression on a live-cell microscope (Incucyte SX5; Sartorius). The optimized protocol is presented in Supplementary Data 2.
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bioRxiv - Cell Biology 2023Quote: ... Cytotoxicity was quantified as reduction of GFP+ area over time via imagining in Incucyte S3 Live-Cell Analysis System (Sartorius).
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... was monitored by imaging every 2 hours for 7 days at 10x magnification with the ‘phase’ channel using the Incucyte live-cell analysis system S3 (Sartorius). Automatic image segmentation and analysis of the phase contrast images was performed by the Incucyte base analysis software (version 2020B).
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bioRxiv - Synthetic Biology 2024Quote: ... The RFP+ count per well was quantified every two hours over a 40-hour span using IncuCyte S3 live-cell imaging (Sartorius). A375 cell growth was calculated as the number of RFP+ objects at a given time point ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Immunology 2023Quote: ... measuring the total fluorophore area per well every 2h h using an Incucyte® S3 Live-Cell Analysis System (Sartorius). The data from each well were normalized to the first measurement (0h).
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation assays of the combination of GEM and clinical ATR inhibitors were performed using the Incucyte live-cell imaging platform (Sartorius). AsPC-1 cells were seeded at 5000 cells/well into 96-well Eppendorf flat bottom plates and allowed to attach overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 x 103 cells/well were plated in FBS-free media in the upper chamber of a 96-well Incucyte ClearView cell migration plate (8 µm pore size; #4582 Sartorius). Cells were incubated at 37°C for 45 minutes to allow cells to settle on the membrane before adding RPMI-1640 media supplemented with 10% FBS to the bottom chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were taken every 15 min for 48 hrs on an Incucyte S3 Live-cell analysis system (Sartorius, 4X/0.2). The area covered by migrating cells was measured with the Incucyte analysis program (Sartorius).
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of confluency was assessed and automatically calculated based on the images acquired with IncuCyte® Zoom Live-Cell Analysis System (Sartorius) both at the day of compound addition (day 0 ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... 100µL of the combined LIVE/DEAD® assay reagent was then added to each well and incubate for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor cells transfected with plasmids containing eGFP were treated and co-cultured as described above in the IncuCyte® SC5 Live-Cell Analysis system (Sartorius). Plates were scanned with a 10x objective using phase contrast channel as well as the green fluorescent channel for 42 h every 6 h ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell brightfield and fluorescent images for time-lapse experiments were acquired using the Incucyte® S3 Live Cell Analysis System (Essen Bioscience / Sartorius) running version 2019A (20191.1.6976.19779) ...
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bioRxiv - Neuroscience 2020Quote: ... Four fluorescent images per well with 300ms red channel acquisition time were taken every 15 minutes in total for three-hours using a 20x objective with the IncuCyte® S3 Live-Cell Analysis System (Sartorius). At the end of the assay ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Microbiology 2021Quote: ... the fixed cells were washed three times with PBS and the cell suspension was filtered with a 5 μm pore size filter (Sartorius, 17594) to remove aggregates ...
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bioRxiv - Microbiology 2023Quote: High cell density fed-batch fermentation for production of Nanobody molecules was performed at 0.25 L (Ambr250 MO units, Sartorius Stedium Biotech), 2 L or 5 L scale (Biostat B-plus and B-DCU units ...
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bioRxiv - Microbiology 2022Quote: ... Fusion events were visualized for 24 h at 20 min intervals using the IncuCyte® S3 Live-Cell Analysis System (Sartorius). Phase contrast and GFP images (4 per well ...
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bioRxiv - Bioengineering 2023Quote: ... COS7 cells at a concentration of 60,000 cells/ml in Dulbecco’s Modified Eagle Medium (DMEM) with 1g/l D-glucose (Sartorius, 01-050-1A), supplemented with fetal bovine serum (Biological Industries ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were spun down at 350g for 10 min at 4°C and placed in the Incucyte® Live-Cell Analysis System (Sartorius). The growth of the spheroids was monitored for the following 8 days and invasion was automatically quantified via the Incucyte® Spheroid module analysis tool ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants were harvested and clarified by centrifugation (3000xg for 20 minutes) or using Sartoclear Dynamics® Lab V (Sartorius). Supernatants were then filtered at 0.2 μm using Nalgene Rapid Flow sterile single using vacuum filter units (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... For the Incucyte assay, THP-1 or Jeko-1 cells were transduced with IncuCyte NucLight Red Lentivirus Reagent (EF-1 Alpha, Puro) (Sartorius, USA). All cell lines were repeatedly tested for the presence of mycoplasma contamination with a MycoReport Mycoplasma Detection Kit (Evrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 mL Tris buffer (50mM, pH 7.6) and afterwards lysed by using a dismembrator (Sartorius, Göttingen, Germany) at 1,600 rpm for 3 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Phase confluence cell images were obtained using a 10× objective lens every 6 hours for 168 hours and the average confluence of each well was calculated with the IncuCyte S3 Live Cell Analysis System (Sartorius, USA).
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bioRxiv - Cell Biology 2024Quote: ... One hundred microliters of the combined LIVE/DEAD® assay reagent was added to each well and incubated for 45 min before quantification of percentage of red and green cells using the IncuCyte® ZOOM (Sartorius) system and its proprietary ZOOM 2016A software.