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Citations for Sartorius :
1 - 50 of 536 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2024Quote: The binding kinetics of ZBP1-Zα1Zα2 with SARS-CoV-2 RNAs were evaluated using the Octet RED96 instrument (Sartorius). First ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Immunology 2020Quote: ... and S309 for binding to soluble SARS-CoV-2 S trimer was assessed using the ForteBio Octet HTX (Sartorius Bioanalytical Instruments). All reagents were diluted to 100 nM in PBSF ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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Mathematical characterization of population dynamics in breast cancer cells treated with doxorubicinbioRxiv - Cancer Biology 2021Quote: ... An IncuCyte S2 Live Cell Analysis System (Essen/Sartorius, Goettingen, Germany) was used to collect fluorescent and phase contrast images every 2-4 hours ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Spike was concentrated using a 100 kDa cut-off concentrator (Sartorius, GE) and loaded onto a HiLoad 16/60 superdex 200 pre-equilibrated with 20 mM Hepes ...
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bioRxiv - Immunology 2020Quote: ... SARS-2pp were concentrated 10-fold using 100,000 MWCO Vivaspin centrifugal concentrators (Sartorius) by centrifugation at 2000xg and stored at –80°C ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Bioengineering 2022Quote: ... Four images were recorded per well every 2 h and analyzed using IncuCyte cell by cell analysis software module (Sartorius). The killing potency of the CAR-T cells was assessed by comparing the percentage of Annexin V positive target cells over time relative to the total number of target cells.
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). Dose-response neutralization curves were analyzed by non-linear regression with a variable slope (GraphPadPrism v8 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed with 2% paraformaldehyde and % GFP positive cells were determined using flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG). Infection was normalized to conditions without antibody ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in 2% paraformaldehyde and GFP positive cells were quantified by flow cytometry (Intellicyt iQue Screener Plus, Sartorius AG). For experiments using single dilutions of serum or ExpiCHO-S cell supernatant ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Microbiology 2022Quote: ... Cells were disrupted by beat beating for 2 minutes using 0.4-0.6 mm glass beads (Sartorius). Beads were pelleted by centrifugation (21,000 × g ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Immunology 2020Quote: ... GFP fluorescent intensity was detected every 2 hours using the IncuCyte S3 Live-Cell analysis system (Sartorius, Germany). Total integrated GFP intensity per well was used as a quantitative measure of viable target cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Parental and MCT1-KO MIA PaCa-2 cells were transduced with Lentiviral NucLight Red and Green vectors (Sartorius), respectively ...
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bioRxiv - Genomics 2023Quote: ... 2% Ultroser-G (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Systems Biology 2023Quote: ... The wells were imaged every 2 hours for 120 hours and cell confluence was measured with Incucyte ZOOM (Sartorius).
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bioRxiv - Cell Biology 2021Quote: ... Brightfield images were taken every 2 hours for 5 days and the percentage confluency calculated using an Incucyte Live-Cell analysis system (Sartorius).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Images and confluence data were acquired every 2 h for ~200 h using the IncuCyte Live Cell Analysis Imaging System (Sartorius). The Area Under Curve (AUC ...