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Citations for Sartorius :
301 - 350 of 519 citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The sterile filtered fecal supernatant was concentrated using centrifugal filters Centrisart with a filter cut-off at 100 kDA (Sartorius) by centrifugation centrifuged at 1,500 x g at 4ºC (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Microbiology 2023Quote: The sterile filtered culture/fecal supernatant was concentrated using centrifugal filters Centrisart with a filter cut-off at 100 kDA (Sartorius) by centrifugation centrifuged at 1,500 x g at 4°C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Microbiology 2023Quote: ... Final separation was achieved following concentrating the Heparin elution to approximately 8 mg/mL using a 30 kDa MWCO Vivaspin filter (Sartorius), by applying the sample to a Superdex 200 10/300 GL (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Flow through of the 0.22 µm filters was subsequently concentrated using tangential flow filtration with a 100 kDa cassette (Sartorius Stedim). The concentrated samples were stored at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C ...
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bioRxiv - Biophysics 2023Quote: Nanodisc samples were concentrated to ∼200 μM in 280 μL in a Vivaspin-6 concentrator with a 30 kDa MWCO (Sartorius). 20 μL D2O was added and gently mixed into the sample ...
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bioRxiv - Synthetic Biology 2022Quote: ... passivated (with 20 μL of 50 mM ABC in 1% sodium deoxycholate or SDC) Vivacon 500 3 kDa molecular weight cutoff (MWCO) filtration units from Sartorius Stedim Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... The monodisperse peak of the complex was collected and concentrated with a 50 kDa cut-off concentrator (Vivaspin 2, Sartorius) and subsequently used for crystallization in LCP.
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bioRxiv - Cell Biology 2023Quote: ... Cell-free supernatants were concentrated to 1 mL by ultrafiltration through a membrane with a molecular weight cut-off of 30 kDa (VivaSpin 20, 30,000 MWCO PES (Sartorius, VS2022)) at 4 °C and 4,000 ˗ g ...
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bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
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bioRxiv - Plant Biology 2024Quote: ... FLVB and FLVA/B were analyzed by SDS-page and then concentrated by ultra filtration (Sartorius, Vivaspin, 10 kDa cutoff). Analysis of different purification steps and quality of protein purifications was also assessed by Immunoblot analysis with custom-made α-FLVA ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...
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bioRxiv - Cell Biology 2024Quote: The filtrate was concentrated to a volume of approximately 30 ml using a tangential flow filtration (TFF) unit with a 100 kDa membrane (Vivaflow 50R, Sartorius) coupled to a 230 V pump (Masterflex) ...
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bioRxiv - Microbiology 2024Quote: The sterile filtered fecal supernatant was concentrated using Centrisart centrifugal filters with a filter cut-off at 100 kDA (Sartorius) by centrifugation at 1,500 x g at 4 °C (dx.doi.org/10.17504/protocols.io.b2qaqdse) ...
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bioRxiv - Biochemistry 2019Quote: ... Human BACE1 was buffer exchanged (in order to remove commercially supplied buffer) prior to spectral analysis using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g washed thrice ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Biochemistry 2021Quote: ... Full-length D-serFS was then concentrated to 200-300 μM using centricons (Vivaspin 500, 10 kDa cutoff, Sartorius Stedim Biotech). For anchoring of optical sensors in brain tissue ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Synthetic Biology 2022Quote: ... cosmids were concentrated and buffer-exchanged against Phosphate Buffered Saline (PBS) by tangential flow filtration (MWCO 100 kDA; Sartorius Vivaflow 200). Packaged cosmid concentration was analyzed by E ...
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bioRxiv - Biochemistry 2019Quote: ... 10 mM DTT was added and further concentrated with a Vivaspin20 100 kDa molecular weight cut off (MWCO) centrifugal concentrator (Sartorius AG). Next ...
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bioRxiv - Biochemistry 2019Quote: ... The main peak fractions were analyzed via SDS-PAGE and further concentrated via a Vivaspin6 100 kDa MWCO centrifugal concentrator (Sartorius AG) until a concentration of 50 µM was reached ...
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bioRxiv - Microbiology 2021Quote: ... using a centrifugation tube with an insert containing a molecular weight cut-off filter (5 kDa, Sartorius Stedim Biotech, Goettingen, Germany). Each HMW fraction was purified by flushing with 20 ml of water ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant was concentrated by ultrafiltration to approximately 70 ml using one 200 cm2 10 kDa MWCO PES tangential flow filtration cassette (Sartorius, Germany) and the ultrafiltration reservoir (Sartorius) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was filtered through a 0.22μm PVDF membrane filter and concentrated to a volume of 500μl at 3320 xg using 100 kDa molecular weight cut off Vivaspin centrifugal ultrafiltration columns (Sartorius, Stonehouse, UK). The resulting filtrate was stored at −80°C until processed for EV isolation.
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteolytic digestion was carried out according to the FASP protocol32 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) using 30 ug of protein resulting from each fraction ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.0 to remove the salt and undesired low molecular weight (LMW) molecules (<10 kDa) from sample using 0.10 m2 Sartocon slice PES cassette (Sartorius Stedim, Germany) and AKTA flux 6 system (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The media was then filtered through a 0.45 µm filter (STARLAB, Hamburg, Germany) and concentrated using a 10 KDa Vivaflow tangential flow filtration (TFF) membrane (Sartorius, Göttingen, Germany) to a volume of 10-15 ml ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated to <500 μl in a centrifugal concentrator with either 30 or 50 kDa molecular weight cut-off (Sartorius VivaSpin).
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bioRxiv - Cell Biology 2023Quote: ... The formulations were then diafiltrated against a 10 × volume of PBS (pH 7.4) through a tangential-flow filtration membrane with a 100 kDa molecular weight cut-off (Sartorius Stedim Biotech), concentrated to the desired concentrations ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Filter-aided sample preparation (FASP) was performed using a 30 kDa molecular weight cut-off filter (VIVACON 500; Sartorius Stedim Biotech). In brief ...
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bioRxiv - Microbiology 2024Quote: ... G3PDH containing fractions as judged by SDS-PAGE and activity measurements were pooled and imidazole was removed using a centrifugal concentrator (10 kDa cutoff, Sartorius, Germany). Protein aliquots were frozen in liquid nitrogen and stored at −80 °C for further use ...
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: All decellularization fractions were digested using the filter aided sample preparation (FASP) protocol as previously described32 using 10 kDa molecular weight cutoff filters (Sartorius, Vivacon #VN01H02). Digests were performed for 16 hours in 25 mM ABC pH 8.0 using trypsin at a 1:100 enzyme:protein ratio at 37°C in an oven ...
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bioRxiv - Biochemistry 2020Quote: ... The peak fractions containing ANT(9) were pooled and concentrated to 6 mg/ml using a Vivaspin 10 kDa cut-off concentrator (Sartorius AG, Germany).
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500, Sartorius AG, Göttingen, Germany), the samples were then brought to comparable protein concentrations and submitted to the Proteomics Facility of the University of Konstanz for protein mass fingerprinting ...
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bioRxiv - Microbiology 2020Quote: ... filtered at 0.22 μm and concentrated to 1 mL using a Vivaspin turbo ultrafiltration units (10 kDa MWCO, Sartorius AG, Goettingen, Germany). Samples were further concentrated to 50 μL after five successive washes using Tris 50 mM ...
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bioRxiv - Biophysics 2022Quote: ... The peak fractions were collected and concentrated to <500 µL using a VivaSpin concentrator with 10 kDa cut-off (Sartorius Stedim Biotech). The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... and fractions containing complete nanodiscs (Fig. S1) pooled and concentrated using a Vivaspin 500 PES 50 kDa cut off spin filter (Sartorius, Cat#VS0131).
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bioRxiv - Molecular Biology 2023Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 30-KDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Vectors are purified by iodixanol step gradient centrifugation and concentrated and buffer-exchanged into Lactated Ringer’s solution (Baxter, Deerfield, IL) using vivaspin20 100 kDa cut-off concentrator (Sartorius Stedim, Goettingen, Germany). Titrations were performed at the transcriptome core facility (Neurocentre Magendie ...
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bioRxiv - Cancer Biology 2020Quote: ... The resultant filtrate was concentrated to a volume of approximately 30 ml using the Vivaflow 50 R tangential flow filtration (TFF) system (Sartorius, MWCO = 100 kDa) and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... and the filtrate concentrated using a ultrafiltration apparatus with a 5 kDa molecular mass cut-off (Vivaflow® 200 cassette, Sartorius Stedim Biotech). The concentrate was separated by reversed phase (RP-HPLC ...
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bioRxiv - Immunology 2022Quote: Virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators with a size exclusion of 100 kDa (Sartorius Stedim Biotech) in order to remove cytokines of lower molecular weight ...
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... Thirty micrograms of protein resulting from each fraction was subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol 20 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethylphosphine) ...