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Citations for Sartorius :
151 - 200 of 519 citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 7.1 mg/ml with a 100-kDa MWCO Vivaspin 2 concentrator (Sartorius), frozen in liquid nitrogen ...
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bioRxiv - Immunology 2021Quote: ... Fractions were desalted and concentrated with 5-kDa cut-off Vivaspin centrifugal filters (Sartorius). Fractionated proteins were separated in 1-D SDS-PAGE in 4-12% Bis-Tris gels ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were pooled and concentrated with Vivaspin 20 concentrators (10 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were transferred into the 30 kDa ultracentrifugal filters (Sartorius Stedim Biotech, Shanghai, China) and washed thrice with 8 M urea and 50 mM triethylammonium bicarbonate buffer (TEAB) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by ultrafiltration at 3-bar with a 100 kDa filter (Sartorius, Goettingen, Germany). Finally ...
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bioRxiv - Immunology 2021Quote: ... Fractions were desalted and concentrated with 5-kDa cut-off Vivaspin centrifugal filters (Sartorius). Fractionated proteins were separated in 1-D SDS-PAGE in 4-12% Bis-Tris gels ...
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bioRxiv - Immunology 2022Quote: ... concentrated via ultra-filtration using Vivaspin 20 centrifugal columns (10 kDa cut-off, Sartorius), and loaded on the SEC column (Superdex 200 26/ 60 ...
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bioRxiv - Microbiology 2022Quote: ... concentrated on a Vivaflow 200 concentration system with a 10 kDa-cutoff membrane (Sartorius). The pH of the concentrated supernatant was adjusted to 8.0 with 100 mM Tris HCl and avidin was added to a final concentration of 1 µg/mL ...
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bioRxiv - Plant Biology 2023Quote: ... The diluted samples were loaded onto filters (Sartorius, Vivacon 500, VN01H22, 30 kDa cutoff) by centrifuging at 14,000 g for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... After two buffer exchanges with trypsin buffer on Vivaspin 15R (5 kDa) columns (Sartorius), the samples were digested by trypsin with 1/80 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: Vivacon 500 columns with a 10 kDA Molecular weight cutoff membrane (Sartorius, Göttingen, Germany) were equilibrated with lysis buffer and centrifuged (14,000× g ...
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bioRxiv - Biophysics 2024Quote: ... Fractions with nanodiscs were concentrated using VivaSpin® 500 MWCO 100 kDa concentrators (Sartorius) to the target concentration (around 2 mg/mL).
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bioRxiv - Systems Biology 2020Quote: ... virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators (Sartorius Stedim Biotech). Virus stocks were stored at −80°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM MgSO4) and concentration of the sample using Vivaspin6 5 kDa MWCO concentrators (Sartorius).
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bioRxiv - Biochemistry 2021Quote: ... Purified enzyme was concentrated (30 kDa cutoff Amicon Stirred Cell, Millipore or VivaSpin4 columns, Sartorius). Protein concentration was determined spectrophotometrically at 280 nm (μCuvette ...
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 30 mg/ml using Vivaspin 20 concentrators (10 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were pooled and concentrated with a Vivaspin 20 concentrator (30 kDa MWCO, Sartorius). The protein was further purified by size exclusion chromatography on a HiLoad 26/600 Superdex 200 pg column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... The solution was concentrated to 70 µL in a 10 kDa cutoff Vivaspin concentrator (Sartorius) and the protein was buffer exchanged with 20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... samples were pooled and concentrated using Vivaspin® 500 centrifugal concentrators (MWCO 50 kDa; Sartorius).
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bioRxiv - Microbiology 2021Quote: ... and 10x concentrated using a tangential flow cassette (100 kDa PES Vivaflow 200, Sartorius, Germany). Approximately 5 mL of the concentrated virome were added to 20 mL of Lysogeny Broth (LB) ...
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bioRxiv - Biochemistry 2019Quote: ... The dialysate was collected and concentrated with a VivaSpin 20 concentrator (10 kDa MWCO, Sartorius) to approximately 1.1 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was purified using centrifugal filtration (cut-off 30 kDa, Vivaspin 500, Sartorius AG, Goettingen, Germany) eight times for 2 min at 15’000 × g at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were desalted and concentrated in 5 kDa cut-off Vivaspin centrifugal filters (Sartorius).
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bioRxiv - Neuroscience 2022Quote: ... The resulting supernatant was concentrated using a Vivaspin device (MWCO, 3 kDa; Sartorius Stedim Biotech) and kept for Western immunoblotting analyses ...
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bioRxiv - Immunology 2022Quote: ... and the eluate was collected into a Vivaspin 6 50 kDa MWCO column (Sartorius, V10631), which was centrifugated at 2,113 g for five minutes to concentrate the antibodies ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... pH 7.4 supplemented with 0.03% DDM using ultrafiltration with MWCO 30 kDa Vivacon 500 (Sartorius) and concentrated to ~10-20 μM ...
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bioRxiv - Microbiology 2023Quote: ... supernatants were sterile filtered and concentrated with 100-kDa centrifugal concentrator cartridges (Vivaspin 20; Sartorius) prior to precipitation with ExoQuickTC and resuspension in phosphate-buffered saline.
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bioRxiv - Microbiology 2023Quote: ... before concentrating and washing the virions by 100 kDa tangential flow filtration (Vivaflow 200, Sartorius). The concentrated viral lysates were sterile-filtered through a 0.22 µm filter (PVDF ...
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bioRxiv - Biochemistry 2024Quote: ... and concentrated over a 3 kDa MWCO membrane using a Vivaspin 15 centrifugal device (Sartorius). The final Ws cytochrome c552 fraction was buffer exchanged to 5 mM Na-phosphate pH 7.0 using a desalting PD-10 column (Cytiva) ...
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bioRxiv - Bioengineering 2024Quote: ... and the remaining supernatant was further concentrated using a 100 kDa Vivaspin 500 filter (Sartorius). The crude small EV suspension was then purified with SEC as previously described.
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bioRxiv - Bioengineering 2024Quote: ... and the resulting supernatant was concentrated using a 100 kDa MWCO Vivaspin 20 filter (Sartorius) at 3,000 ×g to a volume of ∼4 ml ...
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bioRxiv - Biochemistry 2024Quote: ... The mixture was then concentrated using a Vivaspin Turbo Ultrafiltration unit (5 kDa MWCO; Sartorius) and buffer-exchanged to 20 mM tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius), before being washed again and resuspended in complete RPMI medium ...
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bioRxiv - Immunology 2020Quote: ... the supernatant containing virus particles was harvested and filtered with a 0.45 μm syringe filter (Sartorius Stedim Biotech). Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions of interest were pooled and concentrated on a 15 kDa MWCO spin concentrator (Sartorius, #VS2001) and were then loaded onto a Superdex 75 gel filtration column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant was sterile-filtered through a 5-kDa molecular weight cut-off filter (Sartorius Vivaspin® 2 ultrafiltration centrifugal concentrator ...
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bioRxiv - Biophysics 2021Quote: ... Elution was concentrated in Vivaspin® 100,000 kDa molecular weight cut-off (MWCO) centrifugal concentrators (Sartorius) to a volume of ∼800 μL ...
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to ∼15-20 mg/ml using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 7-8 mg/ml using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 12-19 mg/ml using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Plant Biology 2021Quote: ... and loaded onto an ultrafiltration column (Vivaspin 3 or 10 kDa MWCO, PES, Sartorius, Palaiseau, France) for concentration and buffer exchange at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... FASP was performed using a 30 kDa molecular weight cutoff filter (VIVACON 500; Sartorius Stedim Biotech) according to published procedures.52 In brief ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixed media were subsequently concentrated through spin columns with a cutoff of 3 kDa (Vivaspin, Sartorius). Mixed proteins were separated on a 4-20% Mini-PROTEAN TGX precast gel (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... were collected and concentrated using a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius) to a final concentration ∼2.5 mg mL-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... All eluate fractions were combined and concentrated on 300 kDa molecular mass cut-off filters (Sartorius).
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bioRxiv - Biochemistry 2021Quote: ... After pooling and concentrating the fractions by VIVASPIN centrifugal filters (30 kDa cutoff: product Sartorius VS2021), the protein was loaded on heparin HiTRAP column (Cytiva ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... and then concentrated down to ∼40 ml using a 10 kDa Vivaflow 200 filtration device (Sartorius). For CirpA1 ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using a tangential flow filtration with a 100 kDa cut-off (Vivaflow, Sartorius, Germany) to 200 mL ...