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Citations for Sartorius :
251 - 300 of 519 citations for Dengue Virus Subtype 4 Envelope 45 kDa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Vesicle-containing fractions were pooled and concentrated with a 0.5 mL Vivaspin 100 kDa MWCO PES concentrator (Sartorius) to ∼20-40 µL for cryo-EM sample preparation.
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Cell Biology 2024Quote: ... Large volumes of EV containing media was concentrated by using tangential cross flow ultrafiltration (100 kDa MWCO, Sartorius). EVs were collected in a refrigerated ultracentrifuge at 100,000-110,000 xg for 90 min (Optima XPN-100 ultracentrifuge using a SW28 or SW32Ti rotor ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 15,000g for 5 min at 4°C and supernatants were passed through a 4 mm regenerated cellulose filter (4 mm Ø, Sartorius). Samples were then dried under nitrogen flow at 40°C and resuspended in 125 µl of methanol/water 70:30 for subsequent analyses.
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bioRxiv - Microbiology 2022Quote: The filtration efficiency of AF-Fe against virus-loaded aerosols was assessed using a compact experimental setup composed mainly of a polycarbonate filtration holder (Sartorius, Germany) connected to three 1.5 l compressed air tanks (PanGas ...
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bioRxiv - Biophysics 2019Quote: ... purified CysRsaA was buffer exchanged into 50 mM HEPES pH 7.0 and 150 mM NaCl using a 30 kDa MWCO centrifugal concentrator (Sartorius). Overnight labeling of CysRsaA protein (>20 µM ...
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bioRxiv - Biochemistry 2021Quote: ... performed by ultrafiltration through a 10 kDa cut-off polyether sulfone membrane (Vivacell 250, Sartorius Stedim Biotech GmbH, Germany). The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl pH 7.5 by ultrafiltration using 100 kDa molecular weight cut-off (MWCO) spin concentrator tubes (Vivaspin, Sartorius). Protein concentration was then determined using absorbance at 280 nm (ε280 = 14,565 mol−1cm−1 ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.4) and added to 100-kDa molecular mass cutoff Vivaspin 20 centrifugal concentrators (100,000 MWCO, Sartorius AG, Germany). The mixture was centrifuged twice at 2,500 × g for 25 to 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP,10% glycerol) and concentrated using Vivaspin turbo with a 10 kDa molecular mass cutoff concentrator (Sartorius) to a final concentration of 1 mg/mL ...
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bioRxiv - Microbiology 2019Quote: ... and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius). The protein was detected by SDS-PAGE (Supplementary Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... the 10,000xg supernatant was concentrated down to 1.0 ml with a 10 kDa molecular weight cut-off Vivaflow 50 R tangential flow (TFF) device (Sartorius) and a 10 kDa Amicon Ultra-15 centrifugal filter unit (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500 ...
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bioRxiv - Biochemistry 2022Quote: Protein-containing fractions were combined and concentrated to 0.5 ml using a 50-kDa molecular weight cutoff (MWCO) Vivaspin 2 concentrator (Sartorius), and desalted by buffer exchange on a PD MiniTrap G-25 column (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... Purified V-ATPase in buffer containing LMNG was concentrated to 1.9 mg/mL with a 100 kDa MWCO VivaSpin 6 centrifugal concentrator at 2000 ×g for 15 min (Sartorius), followed by a VivaSpin 500 concentrator at 12,000 ×g for 2 min.
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bioRxiv - Molecular Biology 2020Quote: ... and concentrated with Vivaspin 500 centrifugal concentrators with a molecular weight cut-off of 10,000 kDa (Sartorius, Göttingen, Germany). Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... samples were further concentrated to ~1-2 mg/mL with a 100 kDa MWCO Vivaspin 500 centrifugal concentrator (Sartorius) at 12000 ×g ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 by repeated ultracentrifugation using a Vivaspin 500 filter device with a cutoff of 30 kDa (Sartorius) at 4 °C and afterwards concentrated ...
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bioRxiv - Cell Biology 2023Quote: ... Then pre-cleared media was concentrated using Vivaflow® 50R cassette with 100 kDa molecular cut-off (VF05H4, Sartorius). Concentrated media was centrifugated at 100000×g for 4 h ...
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bioRxiv - Microbiology 2023Quote: ... This monodisperse peak was pooled and concentrated to 20 mg/ml with a 10 kDa MWCO ultrafiltration device (Sartorius).
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bioRxiv - Molecular Biology 2023Quote: ... Isolated EVs were subsequently concentrated to a volume of 100 ul using a 2 ml 100,000 kDa Vivaspin column (Sartorius).
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bioRxiv - Cell Biology 2023Quote: ... samples were denatured in UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and centrifuged on a 30 kDa cut-off filter device (PALL or Sartorius) and washed thrice with UA buffer and twice with 50 mM ammoniumbicarbonate (ABC) ...
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bioRxiv - Bioengineering 2023Quote: ... Buffer of AFC eluate was then exchanged by AEX equilibration buffer using 10 kDa NMWCO Vivaspin (Sartorius Stedim, Germany) column then applied in AEX resin ...
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bioRxiv - Microbiology 2023Quote: ... Cytiva) in 50 mM sodium acetate (pH 5.2) and concentrated using Vivaspin polyethersulfone membrane (3 kDa cut-off, Sartorius). Protein concentrations were determined using the Bradford method (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtrate was then concentrated using a tangential flow filter with a 100 kDa membrane (Viva-flow 50R, Sartorius), followed by ultrafiltration using 100 kDa Amicon Ultra-15 units (Milex ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR was initially concentrated to a final concentration of 172 μM via centrifugal ultrafiltration (30-kDa molecular weight cutoff; Sartorius) and then extensively dialyzed against buffer C ...
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bioRxiv - Biophysics 2019Quote: ... three successive 1:30 dilutions were performed to remove unbound dye molecules using a 30 kDa MWCO centrifugal concentrator (Sartorius) and buffer containing 50 mM Tris/HCl pH 8.0 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: The flow through from the above column was concentrated to 3 mL using a 10 kDa centrifugal concentrator (Sartorius Stedim) and loaded onto a pre-equilibrated (with buffer D ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing pure agrin constructs as assessed by SDS-PAGE analysis underwent concentration through a Vivaspin Turbo 30 kDa MWCO concentrator (Sartorius) to reach a final volume below 0.5 mL ...
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bioRxiv - Biochemistry 2020Quote: ... the purified NT-mini/NT*-mini was concentrated to 1 mg/mL with a Vivaspin Turbo10 kDa MWCO concentrator (Sartorius), flash frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Biochemistry 2020Quote: ... were exchanged into phosphate buffer (pH 7.4) using disposable Vivaspin® 500 centrifugal concentrators with an MWCO of 3 kDa (Sartorius) to remove Cu2+ or H202 ...
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bioRxiv - Biophysics 2021Quote: ... and 0.01 mg/mL 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were added before concentration in Vivaspin® 100,000 kDa molecular weight cut-off (MWCO) centrifugal concentrators (Sartorius) to a volume of ∼500 μL ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were analyzed for pure proteins by 12% SDS-PAGE and fractions containing pure enzymes were pooled and concentrated using Vivaspin concentrators with 30 kDa cut-off filter (Sartorius). Protein concentrations were determined using the corresponding extinction coefficient at 280 nm (ExPASy ...
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bioRxiv - Biophysics 2021Quote: ... 0.1 mM EDTA [pH 7.4]) and added to the 100-kDa molecular mass cutoff Vivaspin 20 centrifugal concentrators (100,000 MWCO, Sartorius AG, Germany). The mixture was centrifuged twice at 2,500 × g for 25 to 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the proteins were identified by SDS-PAGE and concentrated using a Vivaspin Turbo 15 centrifuge filter with a 10 kDa molecular weight cutoff (Sartorius). Glycerol was added to a final concentration of 25% ...
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bioRxiv - Plant Biology 2022Quote: ... The eluate (3x 500 μl) was concentrated by ultrafiltration (Vivaspin concentrators, 10 kDa molecular weight cut-off; Sartorius; Göttingen, Germany) and dialyzed against 50 mM Na2HPO4/NaH2PO4 pH 6.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were checked via SDS-PAGE and the cleanest Chz1-containing fraction was concentrated using a 10 kDa cutoff Vivaspin 6 concentrator (Sartorius) at 3,000 g at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... A protein amount of 15 µg was subjected to tryptic protein digestion applying the the filter aided sample preparation protocol (FASP) (Wisniewski et al., 2009) using Vivacon spin filters with a 30 kDa cut-off (Sartorius). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and Ub/LC3B-PA was concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C.
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Synthetic Biology 2020Quote: ... 20 min) followed by cross-flow filtration of the supernatant using a 100 kDa filtration cassette (Hydrosart, Sartorius AG, Germany). EPS was precipitated by slowly pouring the concentrated fermentation supernatant into two volumes of isopropanol ...
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bioRxiv - Immunology 2020Quote: ... The EV pellet was resuspended in 2 ml PBS and stored at −80 prior to further concentration using a 5 kDa MW cut-off vivaspin (Sartorius). The protein content of EVs was quantified using Qubit (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus containing medium was concentrated down to approximately 30 mL using a pump-powered filter (100-kDa cut-off; Vivaflow, Sartorius) and then dialysed into an excess of buffer (10 mM Tris pH 8.0-150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were additionally purified through a Superdex 200 26/60 size exclusion column (Pharmacia Biotech) and concentrated using a Vivaspin Turbo 3 kDa concentrator (Sartorius). Protein concentrations were measured using a UV/Vis spectrophotometer (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... fractions containing FKBP12 F36V were pooled together and concentrated using Vivaspin 20 centrifugal concentrators with a 5 kDa cut-off (Sartorius). The concentration of FKBP12 F36V was determined spectrophotometrically at 280 nm using the calculated molar extinction coefficient ...
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bioRxiv - Microbiology 2023Quote: ... buffer was exchanged to PBS (same volume of the initial serum) using 10 kDa MWCO Vivaspin6 filters (Sartorius, Gӧttingen, Germany) and the resulting preparations were used to measure BG505/T332N neutralization as described above.