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Citations for Sartorius :
301 - 350 of 409 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Tumor fluorescence was monitored every 2–3 h with a ×10 objective using the IncuCyte S3 Live-Cell Analysis System (Sartorius), housed in a cell culture incubator at 37 °C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... the volume was adjusted to 5 mL and transferred to a Centrifugal Concentrator (Vivaspin 6; Sartorius). The retentate was adjusted to 120 µL prior to analysis.
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant was sterile-filtered through a 5-kDa molecular weight cut-off filter (Sartorius Vivaspin® 2 ultrafiltration centrifugal concentrator ...
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bioRxiv - Microbiology 2022Quote: ... centrifugation (10,000 ×g for 5 min) and filtration (0.22-µm membaranefilter; Sartorius Stedim Biotech, Goettingen, Germany).
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bioRxiv - Immunology 2023Quote: ... seeded at 5 × 105 cells/100 μL into Incucyte Imagelock 96-well plates (Sartorius, BA-04857) and incubated for 6 hours ...
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bioRxiv - Microbiology 2023Quote: ... 2 L or 5 L scale (Biostat B-plus and B-DCU units, Sartorius Stedium Biotech) as previously described [PMID ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were grown at 37°C and 5% CO2 in an Incucyte Zoom (Sartorius). Cell death was monitored hourly.
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bioRxiv - Biochemistry 2023Quote: ... concentrated to 5 mg mL-1 using a 100 kDa molecular weight cut off concentrator (Sartorius), and processed immediately for cryo-electron microscopy.
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bioRxiv - Immunology 2024Quote: ... and 1:1 E:T ratios for 5 days in an IncuCyte S3 live cell imager (Sartorius). Wells were imaged every 6 hours ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated with a Vivaspin 20 centrifugal concentrator with a 30,000 Da molecular weight cut-off polyethersulfone membrane (Vivaspin 30k concentrator) (Sartorius, Göttingen, Germany) and injected onto a HiLoad Superdex 75 prep grade size-exclusion column (Cytiva) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Microbiology 2022Quote: ... the pre-cultures were transferred with peristaltic pump into the 02 L (DCI-Biolafitte, US) and 20 L (Sartorius Bbraun, US) bioreactors respectively ...
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bioRxiv - Cell Biology 2024Quote: ... Annealed iMyo01 (final concentration of 0.7 mM) was dissolved in 20 mM sodium phosphate (pH 6.5) using a centrifuge concentrator (Vivaspin 3000 MWCO; Sartorius, Göttingen, Germany). For the K+ condition ...
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bioRxiv - Microbiology 2022Quote: ... Fusion events were visualized for 24 h at 20 min intervals using the IncuCyte® S3 Live-Cell Analysis System (Sartorius). Phase contrast and GFP images (4 per well ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants were harvested and clarified by centrifugation (3000xg for 20 minutes) or using Sartoclear Dynamics® Lab V (Sartorius). Supernatants were then filtered at 0.2 μm using Nalgene Rapid Flow sterile single using vacuum filter units (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... were dialyzed against 20 mM Tris-HCl (pH 8.0) and concentrated to 10-15 mg/ml using Vivaspin Ultra concentrators (Sartorius, Göttingen, Germany). Purity of the proteins was analyzed after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... the secretome also known as conditioned medium (CM) was transferred to a low molecular weight cut-off (5kDa Vivaspin® 20, Sartorius) and centrifuged at 3000×g ...
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bioRxiv - Immunology 2024Quote: ... proteins were buffer exchanged into TN75 (75 mM NaCl, 20 mM Tris HCl pH 8.0) or PBS buffers by ultrafiltration with Vivaspin20 filters (Sartorius, Gӧttingen, Germany) of MWCO 100 kDa ...
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bioRxiv - Immunology 2021Quote: ... 7 L of Sf9 cell culture at a density of 2 × 106 cells/mL were infected with the baculovirus at a MOI of 3 using a Biostat B plus bioreactor (Sartorius, Germany). The following conditions were maintained during the culture period ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... were used to collect air on 37 mm sterile gelatin filters (3 µm pore size; Sartorius, Cat. No. 12602-37-ALK) containing a backing filter to ensure the gelatin filter remained intact during sampling ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
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bioRxiv - Cancer Biology 2023Quote: 2,000 cells per well were plated in 96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network external controller hard drive that gathered and processed image data ...
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bioRxiv - Cancer Biology 2023Quote: Analysis of apoptosis using fluorescence live cell microscopy Cells were stained with Incucyte® Caspase-3/7 Dye for Apoptosis (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cultured in DMEM (10% FBS and 3% glutamine) were grown to 80-90% confluency in Incucyte® ImageLock 96-well plates (Sartorius) and 700-800 µm scratch was made in cells using an Incucyte® 96-Well Woundmaker ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants from control and treated PACs (10 ml each) were concentrated separately to 2 ml with a Vivaspin 20 Ultrafiltration Unit (Sartorius Göttingen, Germany). The resulting samples were then subjected to protein precipitation to reduce the volume and enrich the proteins ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Biophysics 2023Quote: ... proteins were exchanged to labelling buffer (20 mM HEPES, 50 mM NaCl, pH 7.5) using VivaSpin 6 concentrator (Sartorius AG, Gottingen, Germany).
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bioRxiv - Microbiology 2024Quote: ... the filtrate was concentrated using Vivaspin 20 units with a 300 kDa molecular weight cut-off (MWCO) PES membrane (Sartorius, Göettingen, Germany), which was centrifuged (2,500 × g ...
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bioRxiv - Cancer Biology 2024Quote: ... was diluted to 0.3 mg/mL in cold CRF10 and 20 µL was added to the appropriate wells of a cold Incucyte® Imagelock 96-well plate ( BA-04856, Sartorius, USA). The plate was incubated at 37 °C and 5% CO2 for 30 minutes ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Immunology 2021Quote: ... with 5 KDa of nominal molecular weight cutoff (MWCO) was used in a SARTOFLOW Advanced (Sartorius, Germany) tangential flow system ...
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bioRxiv - Immunology 2021Quote: ... Fractions were desalted and concentrated to 0.5 ml with 5-kDa cut-off Vivaspin centrifugal filters (Sartorius). Fractionated proteins were separated by 1-D SDS-PAGE in 4-12% Bis-Tris gels ...
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bioRxiv - Immunology 2021Quote: ... Fractions were desalted and concentrated to 0.5 ml with 5-kDa cut-off Vivaspin centrifugal filters (Sartorius). Fractionated proteins were separated by 1-D SDS-PAGE in 4-12% Bis-Tris gels ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged for 5 minutes at 2000 rpm and then images were acquired using an IncuCyte S3 (Sartorius). Cell viability and caspase 3/7 activity was measured with CellTiter Glo (Promega ...
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bioRxiv - Immunology 2022Quote: Biotinylated OspCA (3 μg/mL) in buffer (PBS containing 2% w/v BSA) was captured onto streptavidin biosensors (#18-5019, Sartorius, Goettingen, Germany) for 5 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... The remaining volume from day 3 onwards was stored at -80 °C for subsequent EPO titer analysis with an Octet RED96 system (ForteBio, Sartorius, Göttingen, Germany). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM CaCl2 pH 8.0 (3 times the original volume) using a 10 kDa molecular weight cut-off centrifugal filter (Vivaspin® Turbo 15; Sartorius, UK), before flash-freezing in liquid nitrogen and storage at −80°C until required.
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bioRxiv - Immunology 2022Quote: Virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators with a size exclusion of 100 kDa (Sartorius Stedim Biotech) in order to remove cytokines of lower molecular weight ...
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... Thirty micrograms of protein resulting from each fraction was subjected to proteolytic digestion using a filter-aided sample preparation (FASP) protocol 20 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02). Samples were reduced with 5 mM tris(2-carboxyethylphosphine) ...
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bioRxiv - Immunology 2024Quote: MPXV clade IIb was isolated from a human specimen collected in the pandemic of 2022 in Berlin and propagated on Vero E6 cells and concentrated using Vivaspin® 20 columns (Sartorius Stedim Biotech). MPXV stocks were diluted in OptiPro serum-free medium complemented with 0.5% gelatine and PBS and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... Samples were held separately in 5 L of sterile marine water (filtered through the 0.22 μm Sartorius filter) for 2 h at 5°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted by centrifugation (16,000g, 5 min) and the supernatant passed through a 0.22 μm nitrocellulose filter (Sartorius). Proteins were precipitated with 10% trichloroacetic acid (TCA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were placed in the Incucyte SX5 and maintained at 37°C and 5% CO2 (Sartorius, Göttingen, Germany) for detection and automated analysis of cell growth/proliferation ...
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bioRxiv - Microbiology 2024Quote: ... samples were clarified using a syringe with a filter cascade (5 µm, 1.2 µm, 0.8 µm, 0.45 µM, Sartorius AG). The cell pellets were not further analysed ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Bioengineering 2024Quote: ... the shake flask culture was centrifuged for 5 min at 110 xg (Model 6K15, Sartorius AG, Göttingen, Germany). The supernatant was discarded and the cell pellet was resuspended in an appropriate volume of medium to generate a stock cell solution with an estimated concentration of 6.6 × 106 cells mL−1 for A and of 2.2 × 107 cells mL−1 for B ...
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bioRxiv - Bioengineering 2023Quote: ... was washed three times in DI water and concentrated to a final volume of 1 mL using 3 kDa MWCO ultrafiltration tubes (Sartorius Stedim Biotech, Germany). The tubes were spun at 4000 rpm for 40 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...