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Citations for Sartorius :
201 - 250 of 409 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and concentrated to the indicated total protein concentration (Vivaspin 20, 30 000 MW cut-off, Sartorius). For depletion assay ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins eluting from the column were concentrated by ultrafiltration on Vivaspin 20 and 2 columns (Sartorius) with a 5 kDa molecular weight cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were concentrated with Vivaspin 20-mL centrifugal devices with 5,000 or 10,000 MWCO (Sartorius) and buffer-exchanged into NMR buffer over PD-10 gel-filtration columns (GE-healthcare).
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bioRxiv - Cell Biology 2023Quote: ... The flow-through containing the peptide was concentrated by centrifugation (Vivaspin 20, 3,000 MWCO PES, Sartorius) and subjected to SEC (Superdex 75 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20, 30 kDa, Sartorius) before injection in a HiLoad 10/300 Superdex S200 Increase gel filtration column (Cytiva ...
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bioRxiv - Physiology 2024Quote: ... Detection was done with the EZ-ECL Chemiluminescence Detection kit for HRP (20-500-120, Sartorius).
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bioRxiv - Microbiology 2024Quote: ... Protein-containing fractions were pooled and concentrated in a Vivaspin 20 centrifugal concentrator (50000 MWCO; Sartorius). Purified complexes were analyzed by spectrophotometry (Nanodrop ...
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bioRxiv - Neuroscience 2024Quote: ... All steps were performed in Kinetics buffer (1X PBS, 0.02% Tween-20, 0.1% BSA, 0.05% sodium azide, pH 7.4, Sartorius) unless otherwise stated ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was transferred to a low molecular weight cut-off (5kDa Vivaspin® 20, Sartorius) and centrifuged at 3,000×g ...
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bioRxiv - Microbiology 2020Quote: ... 5 mL of cultures were sampled and filtered through PTFE membrane (Sartorius) at time points before and after 0.5 mg/mL arginine hydroxamate treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... Plates were analyzed 5 days post seeding using an Incucyte S3 (Sartorius). Additionally ...
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bioRxiv - Microbiology 2024Quote: Infected MRC-5 or ARPE19 cells were place into Incucyte S3 (Sartorius) at 2 hpi ...
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bioRxiv - Plant Biology 2021Quote: ... and loaded onto an ultrafiltration column (Vivaspin 3 or 10 kDa MWCO, PES, Sartorius, Palaiseau, France) for concentration and buffer exchange at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to 3 mg/ml using a 50 kDa cutoff concentrator (Vivaspin, Sartorius). The purified protein was flash frozen in liquid nitrogen and stored at − 80 °C.
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bioRxiv - Immunology 2023Quote: ... they were concentrated using Vivaspin centrifugal concentrators with a cut-off of 3 kDa (Sartorius, VS2091) and stored at -20°C ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants containing the fibrils were recovered and concentrated on vivaspin® 3 KDa (Sartorius, VS04T91) at 3,000 g ...
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bioRxiv - Developmental Biology 2022Quote: ... EpiLC differentiation medium was added and supplemented with 5µM of Caspase 3/7 Green dye (Sartorius) to detect for apoptosis in live cells ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated on a 3-kDa cut-off centrifugal concentrator (nitrocellulose, Vivaspin from Sartorius), and the buffer was exchanged for 25 mM Tris/HCl pH 7.6 ...
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bioRxiv - Systems Biology 2020Quote: ... virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators (Sartorius Stedim Biotech). Virus stocks were stored at −80°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and concentrated 40x by passing through VIVASPIN 20 MWCO 10 kDa centrifugal concentrator columns (Sartorius Stedim Biotech), as per manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... The samples were then concentrated from 40 mL to ∼2 mL using vivaspin 20 ultrafiltration units (Sartorius), and further concentrated to ∼100 µL using vivaspin 2 ultrafiltration units (Sartorius) ...
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Immunology 2024Quote: ... Plates were left at room temperature for 20 min and transferred to the Incucyte SX1 instrument (Sartorius) for baseline images ...
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bioRxiv - Biophysics 2023Quote: ... and added to 100-kDa molecular mass cutoff Vivaspin 20 centrifugal concentrators (100,000 MWCO; Sartorius AG, Germany). The mixture was centrifuged twice at 2,500 g for 25–30 min at 4°C until the supernatant level in the concentrator reached a final volume of 300–350 µL.
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bioRxiv - Cancer Biology 2024Quote: ... concentrated by centrifugation (3000g for 20 min) using a 10 kDa molecular mass cutoff (MWCO) column (Sartorius).
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to 2-3 mg/ml using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diafiltered at 4°C using 3 kDa cutoff VivaSpin®500 centrifuge filters (Sartorius) for 3 cycles of 20 min at 14,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Proteolytic DEVD cleaving activity was determined using the Incucyte Caspase-3/7 Red Dye for Apoptosis (Sartorius) as described in the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Homogenates were centrifuged for 3 min at 17,000g and filtered using a 0.8 μm PES filter (Sartorius). Filtrates were treated with micrococcal nuclease (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: Antibodies were incubated at a ratio of 1:3 antibody to FabFluor red reagent (Sartorius cat# 4722) for 15 minutes at 37 ℃ ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated ~5-fold using a 10 kDa Sartocon Slice ECO Hydrosart cassette (Sartorius), and diafiltered against 4 volumes of 10 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
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bioRxiv - Microbiology 2024Quote: ... The bacterial culture was collected and filtered through a 5 μM filter (Sartorius) to obtain a single-bacteria cell suspension ...
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bioRxiv - Biochemistry 2022Quote: ... The final sample was concentrated to 20 mg/ml with a Vivaspin 2 device (2 kDa cutoff, Sartorius).
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bioRxiv - Immunology 2024Quote: ... Antibodies were diluted to a final concentration of 20 ug/mL before being immobilized on ProteinA tips (Sartorius) for 600 s ...
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bioRxiv - Neuroscience 2022Quote: ... Buffer exchange and concentration was done with Vivaspin 20 ultrafiltration unit having a 1.000.000 molecular weight cut-off (Sartorius). Viral particles were washed and resuspended in PBS and titered using quantitative PCR with primers targeting WPRE element.
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bioRxiv - Biochemistry 2023Quote: ... Fractions that contained BLMcore and no detectable nuclease activity were concentrated in Vivaspin-20 10 kDa concentrator (Sartorius) to 2 mL and loaded onto the HiPrep 16/60 Sephacryl S-300 HR column (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... at 5 μg/mL in 1× kinetics buffer (1× PBS at pH 7.4, and 0.002% v/v Tween 20) were loaded onto ProG biosensors (Sartorius), then incubated with the 40× diluted scFv sample (5 μL of sample added to 195 μL of 1× kinetics buffer) ...
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bioRxiv - Immunology 2024Quote: ... biotinylated H1 or H3 stem construct at 0.5 μM in 1× kinetics buffer (1× PBS at pH 7.4, and 0.002% v/v Tween 20) were loaded onto SA biosensors (Sartorius) and incubated with 33 nM ...
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bioRxiv - Immunology 2024Quote: ... biotinylated H1 or H3 stem construct at 0.5 μM in 1× kinetics buffer (1× PBS at pH 7.4, and 0.002% v/v Tween 20) were loaded onto SA biosensors (Sartorius) and incubated with 9× dilution (20 μL of sample added to 160uL of 1× kinetics buffer ...
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bioRxiv - Cell Biology 2024Quote: ... using Vivaspin 20 PES ultrafiltration units with a molecular weight cut-off (MWCO) of 100 kDa (Sartorius, VS2041). BMVs were isolated by ultracentrifugation at 4 °C and 150,000 x g for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... The viral solution was further concentrated in D-PBS(-) using Vivaspin 20 (100,000 MWCO PES, Sartorius, Gottingen, Germany).
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bioRxiv - Immunology 2024Quote: ... biotinylated natural Ara h 2 (InBio) diluted in kinetics buffer (Dulbecco’s PBS, 1% BSA, 0.02% Tween-20) was loaded onto streptavidin sensors (Sartorius) for 100 seconds ...
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bioRxiv - Microbiology 2021Quote: ... cell culture supernatant was centrifuged for 3 min at 17,000 × g and filtered with a 0.8-μm PES filter (Sartorius). The filtrate was treated with benzonase (Novagen ...
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bioRxiv - Microbiology 2020Quote: ... The homogenates were centrifuged for 3 min at 17,000 g and filtered with 0,8 μm PES filters (Sartorius). Filtrates were treated with benzonase (Novagen ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant (cytosol fraction) was removed and concentrated with Vivaspin 500 Centrifugal Concentrator filters (cutoff, 3 kDa) (Sartorius) as described by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... Finally pure FGF1 was concentrated to 150 µM using a 3 kDa MWCO 500 µL spin concentrator (Sartorius) then snap frozen in liquid nitrogen and stored at −80°C in 200 µL fractions ...
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bioRxiv - Cancer Biology 2024Quote: ... Evaluation of apoptosis was evaluated in both cell lines using IncuCyte® caspase-3/7 reagent (Sartorius, USA) that is non-fluorescent until activated by caspase-3/7 apoptosis activity ...
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bioRxiv - Immunology 2021Quote: ... Fractions were desalted and concentrated with 5-kDa cut-off Vivaspin centrifugal filters (Sartorius). Fractionated proteins were separated in 1-D SDS-PAGE in 4-12% Bis-Tris gels ...