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Citations for Sartorius :
201 - 250 of 279 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... This monodisperse peak was pooled and concentrated to 20 mg/ml with a 10 kDa MWCO ultrafiltration device (Sartorius).
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bioRxiv - Bioengineering 2023Quote: ... Buffer of AFC eluate was then exchanged by AEX equilibration buffer using 10 kDa NMWCO Vivaspin (Sartorius Stedim, Germany) column then applied in AEX resin ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alecto Ephrin B2-hFc and B3-hFc were diluted to 10 µg/mL in 10x Octet kinetics buffer (Sartorius). Ephrins were then loaded onto hydrated anti-Human-Fc capture (AHC ...
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bioRxiv - Microbiology 2024Quote: ... the hydrolysate was filtered with a 10 kDa Vivaflow® 200 10,000 MWCO Hydrosart crossflow cassette (Sartorius, Göttingen, Germany) to remove the protease and larger peptides ...
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bioRxiv - Biochemistry 2024Quote: ... pooled and concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Vivaspin® Turbo 15; Sartorius, UK) prior to being applied to a S75 column pre-equilibrated in 25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... 4h and 24h at 37±0.1°C under agitation (1,400g) in a filtered 1.5ml tube (10-kDa cutoff) from Sartorius (Göttingen, Germany), according to the filter-aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2024Quote: ... Purified Pellino FHA domains were concentrated to 50mg/mL with a 10 kDa molecular weight cut-off concentrator (Sartorius).
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bioRxiv - Biochemistry 2020Quote: ... glycerol was added and the protein concentrated to 20 – 30 mg/mL in a 10 kDa MWCO spin concentrator (Vivaspin, Sartorius).
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bioRxiv - Molecular Biology 2020Quote: ... The resulting CM was then concentrated to approximately 10 ml using the Vivaflow 50R tangential flow device (Sartorius, Göttingen, Germany) with a 100 kDa cut-off membrane ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were quantified by BCA assay (Pierce) and transferred to a 10 kDa MWCO spin filter (Vivaspin 500; Sartorius) before centrifugation at 12,000 x g to collect denatured and reduced proteins atop the filter membrane ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the proteins were identified by SDS-PAGE and concentrated using a Vivaspin Turbo 15 centrifuge filter with a 10 kDa molecular weight cutoff (Sartorius). Glycerol was added to a final concentration of 25% ...
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bioRxiv - Plant Biology 2022Quote: ... The eluate (3x 500 μl) was concentrated by ultrafiltration (Vivaspin concentrators, 10 kDa molecular weight cut-off; Sartorius; Göttingen, Germany) and dialyzed against 50 mM Na2HPO4/NaH2PO4 pH 6.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were checked via SDS-PAGE and the cleanest Chz1-containing fraction was concentrated using a 10 kDa cutoff Vivaspin 6 concentrator (Sartorius) at 3,000 g at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Bioengineering 2020Quote: ... The fractions containing MTG variants were concentrated to a volume of approximately 5 mL using ultrafiltration membrane of 10 kDa MWCO (Vivaspin Turbo 15, Sartorius). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: ... Basolateral compartments were filled with culture medium containing 10% FBS and plates were placed in an Incucyte S3 Imaging System (Sartorius). Images of the apical and basolateral sides of the porous membrane were captured every 4 hours over 3 days ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was concentrated to 10 – 20 mg/mL using a Centrisart I MWCO 100 kDa concentrator (Sartorius AG).
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bioRxiv - Molecular Biology 2021Quote: ... Cellular debris were spun down for 10 min at 9500x g and the concentrate was filtered with a glass fiber prefilter (Minis-art, Sartorius). Protein was purified by Nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Immunology 2022Quote: ... Bench scale production was done in 10 liters bioreactor glass connected to Biostat BDCU controlling system (Sartorius-Stedim, Bohemia, NY). The cells grew in SFX-insect media (Hyclone ...
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bioRxiv - Microbiology 2022Quote: ... The fractions containing the sample of interest were pooled and concentrated using 10 kDa (MtPAPP) and 30 kDa cut-off centrifugal concentrator (Sartorius). The yield of this purification was ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... FLVB and FLVA/B were analyzed by SDS-page and then concentrated by ultra filtration (Sartorius, Vivaspin, 10 kDa cutoff). Analysis of different purification steps and quality of protein purifications was also assessed by Immunoblot analysis with custom-made α-FLVA ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...
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bioRxiv - Biochemistry 2023Quote: ... These clones were incubated until 10-30% confluency and screened for high GFP and RFP signal using Incucyte S3 (Sartorius). The highest expressing monoclonal cells were selected and further expanded on 6 well plates and again screened for fluorescence using the Incucyte ...
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bioRxiv - Microbiology 2023Quote: ... were deposited on a 35 mm x 10 mm polystyrene petri dish (Falcon, Cat. No. 351008) and placed on a microbalance (Sartorius Cubis I ...
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bioRxiv - Microbiology 2023Quote: ... buffer was exchanged to PBS (same volume of the initial serum) using 10 kDa MWCO Vivaspin6 filters (Sartorius, Gӧttingen, Germany) and the resulting preparations were used to measure BG505/T332N neutralization as described above.
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bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biophysics 2023Quote: ... The flow-through volume containing the untagged protein was concentrated to 10 mg/mL using Vivacon500 filters (50 kDa, Sartorius) for further purification using a Superdex 200 Increase 5/150 GL column equilibrated in 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Microbiology 2024Quote: ... A sample of 6 L of air per min was collected during the 10 min exposure on the 47 mm gelatin filter (Sartorius). Post-exposure ...
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bioRxiv - Microbiology 2024Quote: ... the tubes were centrifuged at 4600x g for 10 minutes and the supernatants were double sterile filtered with 0.2 µm pore size Minisart Syringe Filter (Sartorius, Germany), before storage in aliquots at −80 °C until use.
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 8000x g for 10 minutes and sterile filtered twice with 0.2 µm pore size Minisart Syringe Filter (Sartorius, Germany). This process was repeated after an additional incubation with the A ...
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bioRxiv - Biochemistry 2024Quote: ... The eluent was buffer-exchanged to reduce the imidazole concentration to less than 50 mM using a 10 kDa cutoff Vivaspin Centrifugal Concentrator (Sartorius), and subsequently incubated overnight with 2 mg recombinant 6His-tagged 3C protease to cleave the enzyme purification tag ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor fluorescence was monitored every 2–3 h with a ×10 objective using the IncuCyte S3 Live-Cell Analysis System (Sartorius), housed in a cell culture incubator at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Biochemistry 2021Quote: A stock solution of sfGFP-ACE2 was diluted to at 10 μg/ml for immobilization onto High Precision Streptavidin (SAX) biosensors (Sartorius, Germany) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... the pH of the hydroponic culture media of the 5 treatments was measured using a pH meter (PB-10, Sartorius, Germany).
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bioRxiv - Biochemistry 2021Quote: ... Full-length D-serFS was then concentrated to 200-300 μM using centricons (Vivaspin 500, 10 kDa cutoff, Sartorius Stedim Biotech). For anchoring of optical sensors in brain tissue ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant was concentrated by ultrafiltration to approximately 70 ml using one 200 cm2 10 kDa MWCO PES tangential flow filtration cassette (Sartorius, Germany) and the ultrafiltration reservoir (Sartorius) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The 50 ml culture supernatant containing Strep-tagged CDA proteins was concentrated to 1 ml and exchanged to buffer W (100 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, pH 8) using 10 kD cutoff centrifugal filters (Sartorius, Germany) before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... A total 75 ml culture supernatant was concentrated and exchanged with buffer W using 10 kD cutoff centrifugal filters (Sartorius, Germany). A 50 µl of Strep-Tactin®XT agarose beads were added to the concentrated sample in a final volume of 1 ml containing 1x protease inhibitor cocktail and incubated overnight at 4 ℃ ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteolytic digestion was carried out according to the FASP protocol32 with 10 kDa molecular weight cutoff filters (Sartorius Vivacon 500 #VN01H02) using 30 ug of protein resulting from each fraction ...
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bioRxiv - Microbiology 2024Quote: ... G3PDH containing fractions as judged by SDS-PAGE and activity measurements were pooled and imidazole was removed using a centrifugal concentrator (10 kDa cutoff, Sartorius, Germany). Protein aliquots were frozen in liquid nitrogen and stored at −80 °C for further use ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.0 to remove the salt and undesired low molecular weight (LMW) molecules (<10 kDa) from sample using 0.10 m2 Sartocon slice PES cassette (Sartorius Stedim, Germany) and AKTA flux 6 system (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: Sylgard-184™ silicone elastomer kit PDMS pre-polymer was well mixed (5 mins) with curing agent at a 10:1 w/w ratio using a digital balance (Sartorius BP610) prior to vacuum desiccation and casting at various temperatures (60-90°C).
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bioRxiv - Molecular Biology 2023Quote: ... The media was then filtered through a 0.45 µm filter (STARLAB, Hamburg, Germany) and concentrated using a 10 KDa Vivaflow tangential flow filtration (TFF) membrane (Sartorius, Göttingen, Germany) to a volume of 10-15 ml ...