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Citations for Sartorius :
151 - 200 of 279 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... protein samples were concentrated using Vivaspin centrifugal concentrator with a 10 kDa cut-off (Sartorius). Protein concentrations were determined using a Nanodrop Spectrophotometer (ND1000 ...
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bioRxiv - Cell Biology 2021Quote: ... fresh medium was supplemented with 1:10 000 IncuCyte® Cytotox Green Reagent (Sartorius, Göttingen, Germany) to determine dead cells ...
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bioRxiv - Plant Biology 2021Quote: ... and loaded onto an ultrafiltration column (Vivaspin 3 or 10 kDa MWCO, PES, Sartorius, Palaiseau, France) for concentration and buffer exchange at 4 °C ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... and then concentrated down to ∼40 ml using a 10 kDa Vivaflow 200 filtration device (Sartorius). For CirpA1 ...
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bioRxiv - Microbiology 2022Quote: ... thermolithotrophicus was continuously grown in a 10 L fermenter (BIOSTAT® B plus, Sartorius, Göttingen, Germany) under diazotrophic conditions with either 10 mM Na2SO3 or Na2SO4 as sulfur source instead of Na2S ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using a tangential flow filtration with a 100 kDa cut-off (Vivaflow, Sartorius, Germany) to 200 mL ...
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bioRxiv - Molecular Biology 2022Quote: ... Reduced and alkylated samples were loaded onto 10 kDa cut-off filters (Vivacon 500, Sartorius #VN01H02) and centrifuged at 14,000 × g for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... pooled enzyme samples after affinity chromatography were concentrated using centrifugal concentrators (10 kDa cutoff) (Sartorius, Germany) and applied to size exclusion chromatography (Superdex 200 HiLoad 26/60 prep grade column or Superose 6/100 column (Cytavia ...
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bioRxiv - Biophysics 2022Quote: ... The serum was filtered using a 10 kDa molecular weight cut-off spin filter (Sartorius, Germany) before incubation with barcoded probes.
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bioRxiv - Cell Biology 2023Quote: ... Using Vivaspin 500 columns with 30 or 10 kDa molecular weight cut off (Sartorius Stedim Biotech), the buffer was exchanged to 10 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the removal of free dye through 10 kDa Vivaspin® 500 centrifugal concentrators (Sartorius Stedim Biotech ...
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bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
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bioRxiv - Cell Biology 2023Quote: ... transferred onto a 10 kDa MW cut-off filter (Vivacon 500, 10,000 MWCO, Sartorius Cat#VN01H02) and spun at 10’000 g for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... imidazole was removed using a 10 or 30 kDa cut-off centrifugal concentrator (Sartorius, Goettingen, Germany). The concentrated pure Saci_1123 and Saci_0315 proteins were stored in 25 mM PIPES-HCl (pH 6.5) ...
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bioRxiv - Microbiology 2024Quote: ... pooled enzyme samples after affinity chromatography were concentrated using centrifugal concentrators (10 kDa cutoff) (Sartorius, Germany) and applied to a Superose 6/100 column (Cytavia ...
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bioRxiv - Biophysics 2021Quote: ... The eluate from this step was concentrated to 10-15 mL with a Vivaspin® concentrator (Sartorius). Concentrated sample was then exchanged into IEX W buffer (same as IMAC W ...
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bioRxiv - Cancer Biology 2021Quote: ... and concentrated 40x by passing through VIVASPIN 20 MWCO 10 kDa centrifugal concentrator columns (Sartorius Stedim Biotech), as per manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Neuroscience 2020Quote: ... after which conditioned media was collected and concentrated 50-fold using 10 kDa cutoff concentrators (Sartorius #14558502). Protein concentration was measured using the Bradford assay ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing LPMO were pooled and concentrated using ultrafiltration spin-tubes (10 kDa cut-off, Sartorius). The protein-containing fractions were analyzed by SDS-PAGE.
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bioRxiv - Biochemistry 2023Quote: ... S120A-FsC or L182A-FsC were pooled and concentrated using centrifugal concentrators (10 kDa cut-off, Sartorius). The protein concentration was calculated by measuring A280 using Nanodrop and the theoretical extinction coefficient (ε = 14690 M-1 cm-1) ...
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bioRxiv - Microbiology 2024Quote: ... The biotinylated MOW-15-22 RBD was diluted to 10µg/mL in 10× Octet kinetics buffer (Sartorius) and loaded onto hydrated Streptavidin biosensors to 1nm shift ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated by centrifugation (3000g for 20 min) using a 10 kDa molecular mass cutoff (MWCO) column (Sartorius).
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bioRxiv - Plant Biology 2020Quote: ... The eluates were concentrated on Vivaspin 500 10 kDa molecular weight cut-off (MWCO) filter/concentrator columns (Sartorius) and the proteins stored at −20°C (1 to 5 mg ml-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... to 0.1 M Glycine / 10 mM Tris / pH 7.5 using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA ...
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bioRxiv - Molecular Biology 2021Quote: ... injection grade (≥ 10 volume changes) using a Sartocon Slice ECO (30 kDa MWCO, 0.14 m²) PES membrane (Sartorius). Buffer exchanged DS preparations were further adjusted to a concentration of ∼1 mg x mL-1 in 20 mM Tris / 150 mM NaCl / pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were centrifuged at 2000 rpm for 10 minutes and passed through a 0.45 µm syringe filter (Sartorius) to remove debris prior to concentration by ultracentrifugation at 25000 rpm for 2 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... plates were scanned with a 10× objective using the Incucyte ZOOM imaging system (Sartorius, Ann Arbor, MI, USA). Normalized GFP expression (GCU ...
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bioRxiv - Immunology 2023Quote: ... and 37.7H IgG were diluted to concentrations of 10 µg/mL and loaded onto Protein A sensors (Sartorius) to a binding signal of 1.0 nM ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was harvested after removing cells by centrifugation at 2,000 g for 10 min and filtered on a Sartobran P sterile capsule of 0.45 μm (Sartorius). The buffer was then exchanged to buffer A (150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Biochemistry 2023Quote: ... Fractions that contained BLMcore and no detectable nuclease activity were concentrated in Vivaspin-20 10 kDa concentrator (Sartorius) to 2 mL and loaded onto the HiPrep 16/60 Sephacryl S-300 HR column (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6.0) using a centrifugal concentrator with a molecular weight cutoff of 10 kDa (Vivaspin 6, VS0602, Sartorius). The presence of the fucosidase (62 kDa ...
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bioRxiv - Cell Biology 2023Quote: ... The cell-free supernatant (CFS) was concentrated to 10 mL with a 100 kDa ultrafiltration cassette (Sartorius, VF05H4) attached to a peristaltic pump ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were obtained by centrifugation (8,000 g, 10 min) and filtration (0.22 μm filters; Sartorius, Göttingen, Germany). Stx production was tested using Vero cell cytotoxicity assay (supplement) ...
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bioRxiv - Immunology 2020Quote: ... Frames were captured over a period of 24 hrs at 1 hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius). Values from all four regions of each well were pooled and averaged across all three replicates ...
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bioRxiv - Immunology 2020Quote: ... Frames were captured over a period of 24hrs at 1-hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius). Values from all four regions of each well were pooled and averaged across all three replicates ...
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bioRxiv - Biochemistry 2021Quote: ... performed by ultrafiltration through a 10 kDa cut-off polyether sulfone membrane (Vivacell 250, Sartorius Stedim Biotech GmbH, Germany). The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... the 10,000xg supernatant was concentrated down to 1.0 ml with a 10 kDa molecular weight cut-off Vivaflow 50 R tangential flow (TFF) device (Sartorius) and a 10 kDa Amicon Ultra-15 centrifugal filter unit (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... the buffer was exchanged to 50 mM Tris-HCl (pH 7.9) without sucrose using 10 kDa mass cutoff centrifugal filters (Sartorius Vivaspin 500 ...
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bioRxiv - Immunology 2022Quote: ... cell culture supernatants where cleared by centrifugation at 2000 x g for 10 min followed by filtration with a 1.2 μm Minisart NML (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Bioengineering 2022Quote: ... the appropriate volume of stock solution was centrifuged at 3000g at 10 °C in Vivaspin® 20 concentrators (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2) and concentrated to ~1 mg/mL by Vivaspin 20 centrifugal filter unit (MWCO 10 kDa) (Sartorius). Sfp synthase and CoA-conjugated dyes were prepared as previously described54 and used to perform the overnight Fab labeling reaction on ice ...
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bioRxiv - Microbiology 2022Quote: ... the combined clarified fluid was directly concentrated to 10-50 fold by Vivaspin 20 Centrifugal Concentrators (Sartorius, Gottingen, Germany) with PES membrane of 100k MW cut off ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed again with PBS before DMEM + 10% FBS was added with 250nM Incucyte® Cytotox Green (Sartorius) +/- 1 μM Imatinib ...
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bioRxiv - Immunology 2023Quote: ... Anti-HA antibodies were diluted in kinetics buffer (PBS with 0.5% serum bovine albumin and 0.01% Tween) to a final concentration of 10 μg/mL before loading onto protein A biosensors (Sartorius) for 200 s ...