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Citations for Sartorius :
51 - 100 of 279 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 4 μM of 6xHis-tagged ASF1A1-172 was immobilized on nickel-charged tris-nitriloacetic acid (Tris-NTA) biosensor (Sartorius) for the 90 seconds ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent and phase contrast images are collected at intervals of 4 hours or shorter throughout the study in the Incucyte S2 Live Cell Analysis System (Essen/Sartorius) with temperature and environmental control ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were spun at 15,000g for 15 min at 4°C and supernatants were then passed through a regenerated cellulose filter (4mm Ø, Sartorius). Samples were then dried under N2 flow at 40°C and resuspended in 125µl of ice-cold methanol/water 70:30 for subsequent analyses.
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to hIP3R-3 were combined and concentrated to 4 mg/mL using a 100 kDa centrifugal filter (Sartorius). The concentrated sample was then centrifuged at 70,000 rpm using a S100AT rotor (Thermofisher) ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was recorded for up to 7 days every 4 hours by the IncuCyte S3 Live-Cell Analysis System (Sartorius) with a phase-contrast microscope at 10X magnification ...
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bioRxiv - Neuroscience 2020Quote: ... cells and media were collected and this mixture was centrifuged at 3700xg at 4°C to remove debris and then concentrated by ultrafiltration using Vivaflow 200 (Sartorius). The purified AAV particles were collected after running the viral concentrate through an iodixanol column (Opti-Prep density gradient medium ...
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bioRxiv - Immunology 2021Quote: ... The purified Spike and RBD recombinant proteins were then buffer exchanged into sterile PBS by centrifuging at 4000xg for 30 minutes at 4 °C in a 10,000 MWCO Vivaspin centrifugal concentrator (Sartorius, Germany) and stored at –80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... every 4 h (10x objective, 5 fields per well) and were analysed using the Incucyte Live-Cell Analysis System (Sartorius) and are represented as RCU x µm²/Well.
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bioRxiv - Cell Biology 2023Quote: MuSCs were freshly isolated and seeded on 0.2% gelatin-coated 96 well plates at a density of 2,000 cells per well in growing conditions and incubated for 4 days in an IncuCyte SX5 Live-Cell Analysis System (Sartorius) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein was eluted using similar buffer with 300 mM imidazole and concentrated by centrifugation with 4 kDa (AlgF) or 30 kDa (AlgJ and AlgX) cutoff Vivaspin Turbo centrifugal concentrators (Sartorius). His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
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bioRxiv - Immunology 2024Quote: ... The collagen suspension was mixed carefully with the cells in a ratio of 4:1 and 50 µl were added in a 96 well image lock plate (Sartorius) on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the samples were moved back and forth every 4 h for a period of 8 h between the blue light source and the IncuCyte reader (Sartorius) to allow the acquisition of the phase and the red channels ...
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bioRxiv - Microbiology 2024Quote: ... and concentrated by centrifugation at 1500 x g at 4 °C using ultrafiltration devices with a filter cut-off at 100 kDa (Centrisart, Sartorius) [37] ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were further purified by size exclusion chromatography using Superdex-75 or −200 (Cytiva) at 4°C and concentrated by Vivaspin (Sartorius) concentrators at 4°C (Kar9-Bim1 ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were placed between two layers of dampened filter paper (9 cm, Sartorius Grade 292) with 5 ml sterile water ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) culture medium from two 90% confluent 15 cm dishes was filtered through a 1.2 μm filter (Sartorius Stedim Biotech) and applied to a HisTrap column (1 ml bed volume ...
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bioRxiv - Microbiology 2022Quote: ... The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm, Sartorius Stedim Biotech GmbH). The biomass was washed on the filter with 10 mL of 60% methanol and stored at -70 °C until metabolite extraction.
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bioRxiv - Cancer Biology 2022Quote: ... Medium containing DMSO or increasing concentration of test compound was added to each well and spheroid growth was monitored for 4 days post-treatment using the IncuCyte® Spheroid Analysis system (Sartorius). Images from 4 days post-treatment and day 0 were analyzed using the IncuCyte® Spheroid Analysis software module ...
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bioRxiv - Bioengineering 2024Quote: ... Cell debris was removed via centrifugation (5000g, 30 min, 4°C) and filtration of the supernatant via Filtropur 0.2 µm filters (Sartorius, Göttingen, Germany). The soluble protein fraction was then loaded on a HisTrap HP column (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
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bioRxiv - Microbiology 2020Quote: ... liquid phage suspension was centrifuged at 4000 g for 10 min at 4°C and the supernatant was subsequently filter sterilised using a 0.45 μm pore sized membrane filter (Sartorius Minisart®, 16555). The filtrate phage lysate was stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: The purified proteins were concentrated and exchanged to 10 mM sodium citrate buffer (pH 5.0) using Vivaspin® Turbo 4 ultrafiltration units with a 5000 MWCO PES membrane (Sartorius; Cat.# VS04T11). The purity and concentration of each PcaLOOL was assessed by SDS-PAGE and the Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Cell Biology 2024Quote: ... Plates were imaged within a 4-hour interval in a multi-spheroid mode using an Incucyte S3 high-content screening system (Sartorius, Bohemia, NY). Average cyst areas (μm2 ...
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bioRxiv - Neuroscience 2024Quote: ... 3-4 organoids from each hPSCs line were transferred to a 60 mm culture dish containing 4 mL of pre-warmed Earle’s Balanced Salt Solution (EBSS) (Sartorius; 02-010-1A). The organoids were manually dissected into small pieces and transferred into a new 60 mm dish containing 2.5 mL of Enzymatic dissociation solution [composed of 27.3 U/mL papain solution (Worthington ...
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bioRxiv - Immunology 2024Quote: ... Assay cells were fixed with 4% paraformaldehyde (PFA) before microsphere uptake readouts (“phagoscore”) were quantified using the iQue Screener Plus (Sartorius, Göttingen, Germany).
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bioRxiv - Cancer Biology 2023Quote: Real-time calcein intensity was monitored using fluorescence live-cell imaging combined with a phase-contrast setup using the 4×objective (Sartorius AG, Gottingen, Germany). Images were acquired hourly and analyzed using Incucyte software and ImageJ.
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bioRxiv - Cancer Biology 2022Quote: ... Cell proliferation was monitored via time-lapse image acquisition (2 images per well) every 4 hours for up to 96 hours using the IncuCyte(R) ZOOM software (Sartorius, vers. 2016B, 2018A). The initial acquisition was used as 0-hour timepoint ...
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bioRxiv - Immunology 2022Quote: BLI experiments on the IL-9 signaling complex were performed using an Octet Red 96 machine (Sartorius) in kinetics buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... The samples were weight with scales from KERN ALS 220-4 (KERN & SOHN GmbH, Balingen-Frommern, Germany) and Sartorius Cubis®-series (Sartorius AG, Göttingen, Germany). During the extraction process ...
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bioRxiv - Physiology 2023Quote: ... Bundles were lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for >4 h and weighed on a microbalance (Sartorius Cubis Microbalance, Gottingen, Germany). The rate of mH2O2 emission was calculated using a standard curve under the same assay conditions and then normalized to fibre bundle dry weight.
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bioRxiv - Physiology 2024Quote: ... the fibres were lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for > 4 hours and weighed on a microbalance (Sartorius Cubis Microbalance, Gottingen, Germany).
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bioRxiv - Physiology 2024Quote: ... the fibers were lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for >4 hours and weighed on a microbalance (Sartorius Cubis Microbalance, Gottingen Germany). The rate of mH2O2 was calculated from the slope (F/min ...
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bioRxiv - Physiology 2024Quote: ... bundles were collected and lyophilized in a freeze-dryer (Labconco, Kansas City, MO, USA) for ∼4 hours and weighed using a microbalance (Sartorius Cubis Microbalance, Gottingen, Germany). Rate of mH2O2 was calculated using a standard curve in identical assay conditions ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-MC and ES5838 (4 x 104) cells per well were plated in a 24-well plate and placed in an IncucyteS3 incubator (Sartorius, Essen BioScience, Inc. Ann Arbor, MI). Nine images per well were taken every 6 hrs for seven days ...
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bioRxiv - Biochemistry 2020Quote: ... 10 kDa MWCO (Sartorius), connected to the diafiltration reservoir (Sartorius ...
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bioRxiv - Biophysics 2024Quote: ... 10% FBS (Sartorius, Göttingen, Germany), 100 U/mL penicillin (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Pooled fractions were concentrated using 10 kDa Vivaspin concentrators (MWCO 10 kDa, Sartorius, Göttingen, Germany), passed over a HiPrep 26/10 desalting column (Cytiva ...
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bioRxiv - Biochemistry 2020Quote: ... 10 kDa MWCO PES membranes (Sartorius); supplemented by NaCl ...
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bioRxiv - Microbiology 2020Quote: ... MWCO 10 kDa (Sartorius Stedim Biotech) to a maximum final volume of 50 ml ...
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bioRxiv - Microbiology 2021Quote: ... 10 kDa MW cut off (Sartorius) protein concentrator to change the buffer to 150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and concentrated to 10 mg/ml using 10 kDa MWCO spin concentrators (Sartorius). Protein purity and identity were confirmed by detection of a single band by Coomassie-stained SDS-PAGE and by LC-MS/MS to confirm the expected sequence.
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bioRxiv - Microbiology 2024Quote: ... loaded onto 10-kDa filter units (Sartorius), and centrifuged at 12,000 × g at 20°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP,10% glycerol) and concentrated using Vivaspin turbo with a 10 kDa molecular mass cutoff concentrator (Sartorius) to a final concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Clean fractions were pooled and concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 10 K (Sartorius), snap frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg/mL streptomycin (Sartorius #03-031-1B), 10 μg/mL blasticidin S HCl (Gibco #A1113903 ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0 was concentrated to 10 mg.mL-1 using 10 kDa molecular weight cut-off spin filter (Vivaspin® Turbo 15; Sartorius, UK) and screened using commercially available screens ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak fractions were concentrated to 5-10 mg/ml with a 10-kDa cutoff Vivaspin Turbo 15 (Sartorius AG, Göttingen, Germany), frozen in liquid nitrogen ...